Comparison of the 17α-hydroxylase/C17,20-lyase activities of porcine, guinea pig and bovine P450c17 using purified recombinant fusion proteins containing P450c17 linked to NADPH-P450 reductase

Drug Metabolism Reviews

Volumn 39, Issue 2-3, 2007, Pages 289-307

  Shet, Manjunath S ^a   Fisher, Charles W ^b   Tremblay, Yves ^c   Belanger, Alain ^c   Conley, Alan J ^d   Mason, J Ian ^e   Estabrook, Ronald W ^f  

^a PURDUE PHARMA L P   (United States)

^b Pharmaceutical Product Development   (United States)

^c LAVAL UNIVERSITY   (Canada)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF EDINBURGH   (United Kingdom)

^f UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Co expression; Flavodoxin; Hydroxylases; NADPH P450 reductase; P450 fusion proteins; Steroid hydroxylations

Indexed keywords

AMINO ACID; CHOLESTEROL; COMPLEMENTARY DNA; CYTOCHROME P450C17; FLAVODOXIN; HYBRID PROTEIN; PRASTERONE; PREGNENOLONE; PROGESTERONE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE FERRIHEMOPROTEIN REDUCTASE; STEROID 17,20 LYASE; STEROID 17ALPHA MONOOXYGENASE; STEROID HORMONE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CELL GROWTH; COW; DNA MODIFICATION; DRUG METABOLISM; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME PURIFICATION; ENZYME STRUCTURE; GUINEA PIG; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; METABOLIC RATE; NONHUMAN; POLYMERASE CHAIN REACTION; SPECTROPHOTOMETRY; SWINE;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CELL DIVISION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA, RECOMBINANT; GENETIC VECTORS; GUINEA PIGS; MOLECULAR SEQUENCE DATA; NADPH-FERRIHEMOPROTEIN REDUCTASE; PLASMIDS; RECOMBINANT FUSION PROTEINS; SPECIES SPECIFICITY; STEROID 17-ALPHA-HYDROXYLASE; SWINE;

EID: 34548407689     PISSN: 03602532     EISSN: 10979883     Source Type: Journal    
DOI: 10.1080/03602530701468391     Document Type: Article

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