Inorganic-organic hybrid luminescent binary probe for DNA detection based on spin-forbidden resonance energy transfer

Journal of the American Chemical Society

Volumn 129, Issue 28, 2007, Pages 8680-8681

  Martí, Angel A ^a   Puckett, Cindy A ^c   Dyer, Joanne ^a   Stevens, Nathan ^a   Jockusch, Steffen ^a   Ju, Jingyue ^b,d   Barton, Jacqueline K ^c   Turro, Nicholas J ^a,b  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b California Institute of Technology ^*  (United States)

^c California Institute of Technology   (United States)

^d NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5' UNTRANSLATED REGION; ARTICLE; DNA DETERMINATION; ENERGY TRANSFER; FLUORESCENCE; HYBRID; LUMINESCENCE; OLIGONUCLEOTIDE PROBE; SPIN FORBIDDEN RESONANCE ENERGY TRANSFER; STEADY STATE; CHEMISTRY; DNA PROBE; ELECTRON; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC PROCEDURES; METHODOLOGY; SPECTROFLUOROMETRY;

DNA PROBES; ELECTRONS; FLUORESCENCE RESONANCE ENERGY TRANSFER; LUMINESCENCE; MOLECULAR PROBE TECHNIQUES; SPECTROMETRY, FLUORESCENCE;

EID: 34548404780     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0717257     Document Type: Article

References (15)

1. Marras, S. A. E.; Tyagi, S.; Kramer, F. R. Clin. Chim. Acta 2006, 363, 48-60.
2. Mergny, J.-L.; Boutonne, A. S.; Garestier, T.; Belloc, F.; Rougée, M.; Bulychev, N. V.; Koshkin, A. A.; Bourson, J.; Lebedev, A. V.; Valeur, B.; Thuong, N. T.; Hèléne, C. Nucleic Acids Res. 1994, 22, 920-928.
3. Tsuji, A.; Koshimoto, H.; Sato, Y.; Hirano, M.; Sei-lida, Y.; Kondo, S.; Ishibashi, K. Biophys. J. 2000, 78, 3260-3274.
4. Martí, A. A.; Li, X.; Jockusch, S.; Stevens, N.; Li, Z.; Raveendra, B.; Kalachikov, S.; Morozova, I.; Russo, J. J.; Akins, D. L.; Ju, J.; Turro, N. J. Tetrahedron 2007, 63, 3591-3600.
5. Cardullo, R. A.; Agrawal, S.; Flores, C.; Zamecnik, P. C.; Wolf, D. E. Proc. Natl. Acad. Sci. U.S.A. 1988, 85, 8790-8794.
6. Martí, A. A.; Jockusch, S.; Stevens, N.; Ju, J.; Turro, N. J. Acc. Chem. Res. 2007, doi: 10.1021/ar600013q.
7. Mosiman, V.L.; Patterson, B. K.; Canterero, L.; Goolsby, C. L. Cytometry 1997, 30, 151-156.
8. Martí, A. A.; Li, X.; Jockusch, S.; Li, Z.; Raveendra, B.; Kalachikov, S.; Russo, J. J.; Morozova, I.; Puthanveettil, S. V.; Ju, J.; Turro, N. J. Nucleic Acids Res. 2006, 34, 3161-3168.
9. Kalyanasundaram, K. Photochemistry of Polypyridine and Porphyrin Complexes; Academic Press: New York, 1992; pp 106-164.
10. Turro, N. J. Modern Molecular Photochemistry, University Science Books: Sausalito, CA, 1991; pp 338-339.
11. Ru probe/Alexa probe used here: 5′-AAG TTG ATC AAG TTG GT-Ru (or Alexa488)-3′; Cy5-Probe-3: 5′-Cy5-TTC ACT GGA TGA-3′. Other Cy5 probe sequences are in Supporting Information. For synthesis, see: Holmlin, R. E.; Dandliker, P. J.; Barton, J. K. Bioconjugate Chem. 1999, 10, 1122-1130. Details and characterization are in Supporting Information.
12. The procedure to determine the optimum integration time window is described in Supporting Information.
13. Cy5-Probe-3 was selected for TRE since it showed the greatest degree of delayed Cy5 fluorescence.
14. 610 without target).
15. Yang, C. J.; Jockusch, S.; Vincens, M.; Turro, N. J.; Tan, W. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 17278-17283.