More productive in vitro culture of Cryptosporidium parvum for better study of the intra- and extracellular phases

Memorias do Instituto Oswaldo Cruz

Volumn 102, Issue 5, 2007, Pages 567-571

  Perez Cordon G ^a   Marin, C ^a   Romero, D ^a   Rosales, C ^a   Sanchez Moreno M ^a   Rosales, M J ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

Cryptosporidium; Culture; Extracellular stages; In vitro

Indexed keywords

ANIMALIA; BOS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 34548381820     PISSN: 00740276     EISSN: 16788060     Source Type: Journal    
DOI: 10.1590/S0074-02762007005000071     Document Type: Article

References (27)

1. Becher KA, Robertson ID, Fraser DM, Palmer DG, Thompson RCA 2004. Molecular epidemiology of Giardia and Cryptosporidium infections in dairy calves originating from three sources in Western Australia. Vet Parasitol 123: 1-9.
2. Carreno RA, Schinitzler BE, Jeffries AC, Tenter AM, Johnson AM, Barta JR 1999. Cryptosporidium is more closely related to gregarines than to coccidian as shown by phylogenetic analysis of apicomplexan parasites inferred using small-subunit ribosomal RNA gene sequences. Parasitol Res 85: 899-904.
3. Entrala E, Molina JM, Rosales MJ, Sánchez M, Mascaró C 2000. Cryptosporidium parvum: oocysts purification using potassium bromide discontinuous gradient. Vet Parasitol 92: 223-226.
4. Fayer R, Morgan U, Upton SJ 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Inter J Parasitol 30: 1305-1322.
5. Griffin K, Matthai E, Hommel M, Weitz JC, Baxby D, Hart CA 1992. Antigenic diversity among oocysts of clinical isolates of Cryptosporidium parvum. J Protozool Res 2: 97-101.
6. Hamer DH, Ward H, Tzipori S, Pereira MEA, Alroy JP, Keusch GT 1994. Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro. Infect Immun 62: 2208-2210.
7. Hijjawi NS 2003. In vitro cultivation and development of Cryptosporidium in cell culture. In RCA Thompson, A Armson, UM Morgan-Ryan, Cryptosporidium from Molecules to Disease, Elsevier, Amsterdam, p. 233-253.
8. Hijjawi NS, Meloni BP, Morgan UM, Thompson RCA 2001. Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell cultures. Inter J Parasitol 31: 1048-1055.
9. Hijjawi NS, Meloni BP, Ng'anzo M, Ryan UM, Olson ME, Cox PT, Monis PT, Thompson RCA 2004. Complete development of Cryptosporidium parvum in host cell-free culture. Inter J Parasitol 34: 769-777.
10. Hijjawi NS, Meloni BP, Ryan UM, Olson ME, Thompson RCA 2002. Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium. Intern J Parasitol 32: 1719-1726.
11. Lacharme L, Villar V, Rojo-Vazquez FA, Suárez S 2004. Complete development of Cryptosporidium parvum in rabbit chondrocytes (VELI cells). Microbes Infection 6: 566-571.
12. Leander BS, Clopton RE, Keeling PJ 2003. Phylogeny of gregarines (Apicomplexa) as inferred from small-subunitrDNA and beta-tubulin. Int J Syst Evol Microbiol 53: 345-354.
13. Maillot C, Favennec L, Francois A, Ducrotte P, Brasseur PJ 1997. Sexual and asexual development of Cryptosporidium parvum in five oocysts or sporozoite infected human enterocytic cell lines. Eukaryot Microbiol 44: 582-585.
14. McDonald V, Deer RMA, Nina JMS, Wright S, Chiodini PL, McAdam KPWJ 1991. Characterization and specificity of hybridoma antibodies against oocyst antigens of Cryptosporidium parvum from man. Parasite Immunol 13: 251-259
15. Monis PT, Thompson RCA 2003. Cryptosporidium and Giardia-zoonoses: fact or fiction? Infect Gen Evol 3: 233-244.
16. Pérez-Cordón G, Rosales MJ, Sánchez-Moreno M 2005. Processing of fecal simples for study of Cryptosporidium sp. by PCR. Rev Peru Biol 12: 158-160.
17. Ramirez E, Ward LA, Sreevatsan S 2004. A review of the biology and epidemiology of cryptosporidiosis in humans and animals. Microbes Infection 6: 773-785.
18. Robertson LJ, Campbell AT, Smith HV 1993. In vitro excystation of Cryptosporidium parvum. Parasitology 106: 13-19.
19. Rosales MJ, Cifuentes J, Mascaró C 1993. Cryptosporidium parvum: culture in MDCK cells. Exper Parasitol 76: 209-212.
20. Rosales MJ, Mascaró C, Arnedo T, Castilla JJ 1994. Isolation and identification of Cryptosporidium parvum oocysts with continuous percoll gradients and combined alcian blue-giemsa staining. Acta Trop 56: 371-373.
21. Rosales MJ, Peréz-Cordón G, Sánchez Moreno M, Marín Sánchez C 2005. Extracellular like-gregarine stages of Cryptosporidium parvum. Acta Trop 95: 74-78.
22. Slapeta J 2006. Cryptosporidium species found in cattle: a proposal for a new species. Trends Parasitol 22: 469-474.
23. Upton SJ, Tilley M, Brillhart DB 1994. Comparative development of Cryptosporidium parvum (Apicomplexa) in 11 continous host cell lines. FEMS Microbiol Lett 118: 233-236.
24. Xiao L, Escalante L, Yang C, Sulaiman I, Escalante A, Montali R, Fayer R, Altaf A 1999a. Phylogenetic analysis of Cryptosporidium parasites based on the small subunit rRNA gene locus. Appl Environ Microbiol 65: 1578-1583.
25. Xiao L, Morgan U, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson R, Fayer R Altaf A 1999b. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol 65: 3386-3391.
26. Ward LA, Wang Y 2001. Rapad methods to isolate Cryptosporidium from frozen feces for PCR. Diag Microbiol Infec Dis 41: 37-42.
27. Yang S, Healey MC, Chunwei Zhang J 1996. Complete development of Cryptosporidium parvum in bovine fallopian tube epithelial cells. Infect Immun 64: 349-354.