Development and validation of an HPTLC method for simultaneous estimation of excitatory neurotransmitters in rat brain

Journal of Liquid Chromatography and Related Technologies

Volumn 30, Issue 19, 2007, Pages 2891-2902

  Babu, C Saravana ^a   Sunil, A G ^a   Vasanthi, Hannah R ^a   Muthusamy, V S ^b   Ramanathan, M ^c  

^a SRI RAMACHANDRA UNIVERSITY   (India)

^b ANNA UNIVERSITY   (India)

^c PSG COLLEGE OF PHARMACY   (India)

Author keywords

Cerebral stroke; HPTLC; L Aspartic acid; L Glutamic acid; Rat brain tissue

Indexed keywords

AMINO ACIDS; BIOELECTRIC POTENTIALS; BRAIN; PHYSIOLOGICAL MODELS; TISSUE ENGINEERING;

CEREBRAL STROKE; L-ASPARTIC ACID; L-GLUTAMIC ACID; RAT BRAIN TISSUES;

NEUROLOGY;

ASPARTIC ACID; GLUTAMIC ACID;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY; MALE; NERVE EXCITABILITY; NEUROTRANSMITTER RELEASE; NONHUMAN; PROTEIN DETERMINATION; RAT; REPRODUCIBILITY; ULTRAVIOLET SPECTROPHOTOMETRY; VALIDATION PROCESS;

MAMMALIA; RATTUS;

EID: 34548314141     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070701588760     Document Type: Article

References (14)

1. Fonnum, F. Glutamic acid: a neurotransmitter in mammalian brain. J. Neurochem. 1984, 42, 1-11.
2. McDonald, J.W.; Johnston, M.V. Physiological and pathophysiological roles of excitatory amino acids during central nervous system development. Brain Res. Rev. 1990, 15, 41-70.
3. Rothman, S.M.; Olney, J.W. Glutamic acid and the pathophysiology of hypoxic/ischemic brain damage. Ann. Neurol. 1986, 19, 105-111.
4. Benvensie, H. The excitotoxin hypothesis in relation to cerebral ischemia. Cerebroasc. Brain Metab. Rev. 1991, 3, 213-245.
5. Choi, D.W. Excitotoxic cell death. J. Neurobiol. 1992, 23, 1262-1276.
6. Sieojo, B.K. Pathophysiology and treatment of focal cerebral ischemia. I. Pathophysiology. J. Neurosurg. 1992, 77, 169-184.
7. Matsumoto, K.; Graf, R.; Rosner, G. Elevation of neuroactive substances in the cortex of cats during prolonged focal ischemia. J. Cereb. Blood Flow Metab. 1993, 13, 586-594.
8. Tsuyuki, Y.U.; Araki, H.; Yae, T. Changes in the extracellular concentrations of amino acids in the rats striatum during transient focal cerebral ischemia. J. Neurochem. 1994, 62, 1074-1078.
9. Lund, P. L-Glutamine and L-gluatmate: UV-method with glutaminase and glutamic acid dehydrogenase. In Methods of Enzymatic Analysis; Bergmeyer, U. Ed.; VCH Verlagsgesellschaft: Weinheim, 1986, 357-363.
10. Bert, L.; Robert, F.; Denoroy, L.; Stoppini, L.; Renaud, B. Enhanced temporal resolution for the microdialysis monitoring of catecholamines and excitatory amino acids using capillary electrophoresis with laser-induced fluorescence detection. Analytical developments and in vitro validations. J. Chromatogr. A 1996, 755, 99-111.
11. Moghaddam, B. Stress preferentially increases extraneuronal levels of excitatory amino acids in the prefrontal cortex: comparison to hippocampus and basal ganglia. J. Neurochem. 1993, 60, 1650-1657.
12. Raju, T.R.; Kutty, B.M.; Sathyaprabha, T.N.; Shankaranarayanan Rao, B.S. Estimation of neurotransmitters in the brain by chromatogarphic methods. In Brain and Behavior; National Institute of Mental Health and Neuroscience: Bangalore, India, 2004, 134-141.
13. Glowinsky, J.; Iversen, L. Regional studies of catecholamines in the rat brain-(I). J. Neurochem. 1966, 13, 655-669.
14. Shankaranarayana Rao, B.S.; Raju, T.R.; Meti, B.L. Self-stimulation of lateral hypothalamus and ventral tegmentum increases the levels of noradrenaline, dopamine, glutamate and AChE activity, but not 5-hydroxytryptamine and GABA levels in hippocampus and motor cortex. Neurochem. Res. 1998, 23 (8), 1053-1059.