Lack of electricity production by Pelobacter carbinolicus indicates that the capacity for Fe(III) oxide reduction does not necessarily confer electron transfer ability to fuel cell anodes

Applied and Environmental Microbiology

Volumn 73, Issue 16, 2007, Pages 5347-5353

  Richter, Hanno ^a   Lanthier, Martin ^a   Nevin, Kelly P ^a   Lovley, Derek R ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHARGE TRANSFER; IRON OXIDES; METABOLISM; MICROBIAL FUEL CELLS; REDOX REACTIONS;

FUEL CELL ANODES; METABOLIC INTERMEDIATE ACETATE;

BACTERIA;

ACETIC ACID; ALCOHOL; FERRIC OXIDE; HYDROGEN; RNA 16S;

BACTERIUM; ELECTRICITY GENERATION; ETHANOL; FUEL CELL; IRON OXIDE; OXIDATION; REDUCTION;

ARTICLE; BACTERIAL METABOLISM; CONFOCAL MICROSCOPY; ELECTRIC CURRENT; ELECTRON TRANSPORT; GEOBACTER; GEOBACTER SULFURREDUCENS; NONHUMAN; OXIDATION REDUCTION REACTION; PELOBACTER; PELOBACTER CARBINOLICUS; RNA ANALYSIS;

BACTERIOLOGICAL TECHNIQUES; DELTAPROTEOBACTERIA; ELECTRICITY; ELECTRON TRANSPORT; FERROUS COMPOUNDS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, CONFOCAL; OXIDATION-REDUCTION; RNA, RIBOSOMAL, 16S;

GEOBACTER SULFURREDUCENS; PELOBACTER; PELOBACTER CARBINOLICUS;

EID: 34548274490     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00804-07     Document Type: Article

References (38)

1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Bond, D. R., D. E. Holmes, L. M. Tender, and D. R. Lovley. 2002. Electrodereducing microorganisms that harvest energy from marine sediments. Science 295:483-485.
3. Bond, D. R., and D. R. Lovley. 2003. Electricity production by Geobacter sulfurreducens attached to electrodes. Appl. Environ. Microbiol. 69:1548-1555.
4. Bond, D. R., and D. R. Lovley. 2005. Evidence for involvement of an electron shuttle in electricity generation by Geothrix fermentans. Appl. Environ. Microbiol. 71:2186-2189.
5. Caccavo, F., Jr., D. J. Lonergan, D. R. Lovley, M. Davis, J. F. Stolz, and M. J. McInerney. 1994. Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl. Environ. Microbiol. 60:3752-3759.
6. Chaudhuri, S. K., and D. R. Lovley. 2003. Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nat. Biotechnol. 21:1229-1232.
7. Coppi, M. V., C. Leang, S. J. Sandler, and D. R. Lovley. 2001. Development of a genetic system for Geobacter sulfurreducens. Appl. Environ. Microbiol. 67:3180-3187.
8. Coppi, M. V., R. A. O'Neil, and D. R. Lovley. 2004. Identification of an uptake hydrogenase required for hydrogen-dependent reduction of Fe(III) and other electron acceptors by Geobacter sulfurreducens. J. Bacteriol. 186:3022-3028.
9. Daims, H., A. Bruhl, R. Amann, K. H. Schleifer, and M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22:434-444.
10. Fuchs, B. M., F. O. Glockner, J. Wulf, and R. Amann. 2000. Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol. 66:3603-3607.
11. Haveman, S. A., D. E. Holmes, Y. H. Ding, J. E. Ward, R. J. Didonato, Jr., and D. R. Lovley. 2006. c-Type cytochromes in Pelobacter carbinolicus. Appl. Environ. Microbiol. 72:6980-6985.
12. Holmes, D. E., D. R. Bond, and D. R. Lovley. 2004. Electron transfer by Desulfobulbus propionicus to Fe(III) and graphite electrodes. Appl. Environ. Microbiol. 70:1234-1237.
13. Holmes, D. E., D. R. Bond, R. A. O'Neil, C. E. Reimers, L. R. Tender, and D. R. Lovley. 2004. Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments. Microb. Ecol. 48:178-190.
14. Holmes, D. E., S. K. Chaudhuri, K. P. Nevin, T. Mehta, B. A. Methe, A. Liu, J. E. Ward, T. L. Woodard, J. Webster, and D. R. Lovley. 2006. Microarray and genetic analysis of electron transfer to electrodes in Geobacter sulfurreducens. Environ. Microbiol. 8:1805-1815.
15. Holmes, D. E., K. P. Nevin, and D. R. Lovley. 2004. Comparison of 16S rRNA, nifD, recA, gyrB, rpoB and fusA genes within the family Geobacteraceae fam. nov. Int. J. Syst. Evol. Microbiol. 54:1591-1599.
16. Holmes, D. E., K. P. Nevin, R. A. O'Neil, J. E. Ward, L. A. Adams, T. L. Woodard, H. A. Vrionis, and D. R. Lovley. 2005. Potential for quantifying expression of the Geobacteraceae citrate synthase gene to assess the activity of Geobacteraceae in the subsurface and on current-harvesting electrodes. Appl. Environ. Microbiol. 71:6870-6877.
17. Holmes, D. E., J. S. Nicoll, D. R. Bond, and D. R. Lovley. 2004. Potential role of a novel psychrotolerant member of the family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in electricity production by a marine sediment fuel cell. Appl. Environ. Microbiol. 70:6023-6030.
18. Kim, H. J., H. S. Park, M. S. Hyun, I. S. Chang, M. Kim, and B. H. Kim. 2002. A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. Enzyme Microb. Technol. 30:145-152.
19. Lanthier, M., P. Juteau, F. Lepine, R. Beaudet, and R. Villemur. 2005. Desulfitobacterium hafniense is present in a high proportion within the biofilms of a high-performance pentachlorophenol-degrading, methanogenic fixed-film reactor. Appl. Environ. Microbiol. 71:1058-1065.
20. Lonergan, D. J., H. L. Jenter, J. D. Coates, E. J. Phillips, T. M. Schmidt, and D. R. Lovley. 1996. Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria. J. Bacteriol. 178:2402-2408.
21. Lovley, D. R. 2006. Bug juice: harvesting electricity with microorganisms. Nat. Rev. Microbiol. 4:497-508.
22. Lovley, D. R., F. H. Chapelle, and J. C. Woodward. 1994. Use of dissolved H2 concentrations to determine distribution of microbially catalyzed redox reactions in anoxic groundwater. Environ. Sci. Technol. 28:1205-1210.
23. Lovley, D. R., R. C. Greening, and J. G. Ferry. 1984. Rapidly growing rumen methanogenic organism that synthesizes coenzyme M and has a high affinity for formate. Appl. Environ. Microbiol. 48:81-87.
24. Lovley, D. R., and E. J. Phillips. 1988. Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl. Environ. Microbiol. 54:1472-1480.
25. Lovley, D. R., E. J. Phillips, and D. J. Lonergan. 1989. Hydrogen and formate oxidation coupled to dissimilatory reduction of iron or manganese by Alteromonas putrefaciens. Appl. Environ. Microbiol. 55:700-706.
26. 0 reduction by Pelobacter carbinolicus. Appl. Environ. Microbiol. 61:2132-2138.
27. Loy, A., A. Lehner, N. Lee, J. Adamczyk, H. Meier, J. Ernst, K. H. Schleifer, and M. Wagner. 2002. Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment. Appl. Environ. Microbiol. 68:5064-5081.
28. Manz, W., K. Wendt-Potthoff, T. R. Neu, U. Szewzyk, and J. R. Lawrence. 1999. Phylogenetic composition, spatial structure, and dynamics of lotic bacterial biofilms investigated by fluorescent in situ hybridization and con-focal laser scanning microscopy. Microb. Ecol. 37:225-237.
29. Mehta, T., M. V. Coppi, S. E. Childers, and D. R. Lovley. 2005. Outer membrane c-type cytochromes required for Fe(III) and Mn(IV) oxide reduction in Geobacter sulfurreducens. Appl. Environ. Microbiol. 71:8634-8641.
30. Pham, C. A., S. J. Jung, N. T. Phung, J. Lee, I. S. Chang, B. H. Kim, H. Yi, and J. Chun. 2003. A novel electrochemically active and Fe(III)-reducing bacterium phylogenetically related to Aeromonas hydrophila, isolated from a microbial fuel cell. FEMS Microbiol. Lett. 223:129-134.
31. Reguera, G., K. D. McCarthy, T. Mehta, J. S. Nicoll, M. T. Tuominen, and D. R. Lovley. 2005. Extracellular electron transfer via microbial nanowires. Nature 435:1098-1101.
32. Reguera, G., K. P. Nevin, J. S. Nicoll, S. F. Covalla, T. L. Woodard, and D. R. Lovley. 2006. Biofilm and nanowire production leads to increased current in Geobacter sulfurreducens fuel cells. Appl. Environ. Microbiol. 72:7345-7348.
33. Schink, B. 1984. Fermentation of 2,3-butanediol by Pelobacter carbinolicus sp. nov., and Pelobacter propionicus sp. nov., and evidence for propionate formation from C2 compounds. Arch. Microbiol. 137:33-41.
34. Seitz, H. J., B. Schink, and R. Conrad. 1988. Thermodynamics of hydrogen metabolism in methanogenic cocultures degrading ethanol or lactate. FEMS Microbiol. Lett. 55:119-124.
35. Seop, C. I., H. Moon, O. Bretschger, J. K. Jang, H. I. Park, K. H. Nealson, and B. H. Kim. 2006. Electrochemically active bacteria (EAB) and mediatorless microbial fuel cells. J. Microbiol. Biotechnol. 16:163-177.
36. Tender, L. M., C. E. Reimers, H. A. Stecher III, D. E. Holmes, D. R. Bond, D. A. Lowy, K. Pilobello, S. J. Fertig, and D. R. Lovley. 2002. Harnessing microbially generated power on the seafloor. Nat. Biotechnol. 20:821-825.
37. Wallner, G., R. Amann, and W. Beisker. 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14:136-143.
38. Wilhelm, E., R. Battino, and R. J. Wilcox. 1977. Low-pressure solubility of gases in liquid water. Chem. Rev. 77:219-262.