Single cell derived murine embryonic stem cell clones stably express Rex1-specific green fluorescent protein and their differentiation study

Biochemical and Biophysical Research Communications

Volumn 362, Issue 2, 2007, Pages 467-473

  Chen, Xiaopan ^a   Wu, Rongrong ^a   Feng, Shumei ^a   Gu, Bin ^a   Dai, Licheng ^b   Zhang, Ming ^a   Zhao, Xiaoli ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b HUZHOU CENTRAL HOSPITAL   (China)

Author keywords

Differentiation; Embryoid body; Mouse embryonic stem cells; Rex1; Transfection

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; PROTEIN REX1; REX PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL DIFFERENTIATION; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; GENETIC TRANSFECTION; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; CLONE CELLS; EMBRYONIC STEM CELLS; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; KARYOTYPING; MICE; MICE, NUDE; MICE, SCID; MICROSCOPY, FLUORESCENCE; NEOPLASMS, EXPERIMENTAL; PROMOTER REGIONS (GENETICS); REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TERATOMA; TRANSCRIPTION FACTORS; TRANSFECTION;

MURINAE;

EID: 34548267108     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.08.008     Document Type: Article

References (26)

1. Evans M.J., and Kaufman M.H. Establishment in culture of pluripotential cells from mouse embryos. Nature 292 (1981) 154-156
2. Martin G.R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 78 (1981) 7634-7638
3. Williams R.L., Hilton D.J., Pease S., Willson T.A., Stewart C.L., Gearing D.P., Wagner E.F., Metcalf D., Nicola N.A., and Gough N.M. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336 (1988) 684-687
4. Johkura K., Cui L., Asanuma K., Okouchi Y., Ogiwara N., and Sasaki K. Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells. J. Anat. 205 (2004) 247-255
5. Lin T., Chao C., Saito S., Mazur S.J., Murphy M.E., Appella E., and Xu Y. p53 induces differentiation of mouse embryonic stem cells by suppressing Nanog expression. Nat. Cell Biol. 7 (2005) 165-171
6. Pyle A.D., Lock L.F., and Donovan P.J. Neurotrophins mediate human embryonic stem cell survival. Nat. Biotechnol. 24 (2006) 344-350
7. Ying Q.L., Nichols J., Chambers I., and Smith A. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell 115 (2003) 281-292
8. Nichols J., Zevnik B., Anastassiadis K., Niwa H., Klewe-Nebenius D., Chambers I., Scholer H., and Smith A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95 (1998) 379-391
9. Chambers I., Colby D., Robertson M., Nichols J., Lee S., Tweedie S., and Smith A. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113 (2003) 643-655
10. Chambers I. The molecular basis of pluripotency in mouse embryonic stem cells. Cloning Stem Cells 6 (2004) 386-391
11. Hosler B.A., LaRosa G.J., Grippo J.F., and Gudas L.J. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells. Mol. Cell Biol. 9 (1989) 5623-5629
12. Eiges R., Schuldiner M., Drukker M., Yanuka O., Itskovitz-Eldor J., and Benvenisty N. Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Curr. Biol. 11 (2001) 514-518
13. Rogers M.B., Hosler B.A., and Gudas L.J. Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes. Development 113 (1991) 815-824
14. Thompson J.R., and Gudas L.J. Retinoic acid induces parietal endoderm but not primitive endoderm and visceral endoderm differentiation in F9 teratocarcinoma stem cells with a targeted deletion of the Rex-1 (Zfp-42) gene. Mol. Cell Endocrinol. 195 (2002) 119-133
15. Ben-Shushan E., Thompson J.R., Gudas L.J., and Bergman Y. Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site. Mol Cell Biol 18 (1998) 1866-1878
16. Mountford P., Nichols J., Zevnik B., O'Brien C., and Smith A. Maintenance of pluripotential embryonic stem cells by stem cell selection. Reprod. Fertil. Dev. 10 (1998) 527-533
17. Asahina K., Fujimori H., Shimizu-Saito K., Kumashiro Y., Okamura K., Tanaka Y., Teramoto K., Arii S., and Teraoka H. Expression of the liver-specific gene Cyp7a1 reveals hepatic differentiation in embryoid bodies derived from mouse embryonic stem cells. Genes Cells 9 (2004) 1297-1308
18. Samokhvalov I.M., Samokhvalova N.I., and Nishikawa S. Cell tracing shows the contribution of the yolk sac to adult haematopoiesis. Nature 446 (2007) 1056-1061
19. Wirth M., Bode J., Zettlmeissl G., and Hauser H. Isolation of overproducing recombinant mammalian cell lines by a fast and simple selection procedure. Gene 73 (1988) 419-426
20. Marone M., Mozzetti S., De Ritis D., Pierelli L., and Scambia G. Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol. Proced. Online 3 (2001) 19-25
21. Doetschman T.C., Eistetter H., Katz M., Schmidt W., and Kemler R. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87 (1985) 27-45
22. Heo J., Lee J.S., Chu I.S., Takahama Y., and Thorgeirsson S.S. Spontaneous differentiation of mouse embryonic stem cells in vitro: characterization by global gene expression profiles. Biochem. Biophys. Res. Commun. 332 (2005) 1061-1069
23. Itskovitz-Eldor J., Schuldiner M., Karsenti D., Eden A., Yanuka O., Amit M., Soreq H., and Benvenisty N. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med. 6 (2000) 88-95
24. Oyamada Y., Komatsu K., Kimura H., Mori M., and Oyamada M. Differential regulation of gap junction protein (connexin) genes during cardiomyocytic differentiation of mouse embryonic stem cells in vitro. Exp. Cell Res. 229 (1996) 318-326
25. Cenkci B., Petersen J.L., and Small G.D. REX1, a novel gene required for DNA repair. J. Biol. Chem. 278 (2003) 22574-22577
26. Huuskonen H. New models and molecular markers in evaluation of developmental toxicity. Toxicol. Appl. Pharmacol. 207 (2005) 495-500