Novel xylan-binding properties of an engineered family 4 carbohydrate-binding module

Biochemical Journal

Volumn 406, Issue 2, 2007, Pages 209-214

  Cicortas Gunnarsson, Lavinia ^a   Montanier, Cedric ^b   Tunnicliffe, Richard B ^c   Williamson, Mike P ^c   Gilbert, Harry J ^b   Nordberg Karlsson, Eva ^a   Ohlin, Mats ^a  

^a LUND UNIVERSITY   (Sweden)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Aromatic residue; Binding specificity; Carbohydrate binding module; Molecular engineering; Thermodynamics; Xylan

Indexed keywords

AROMATIC RESIDUE; BINDING SPECIFICITY; CARBOHYDRATE-BINDING MODULE; MOLECULAR ENGINEERING; XYLAN;

ENZYMES; HYDROPHOBICITY; LIGANDS; OLIGOMERS; POLYSACCHARIDES; THERMODYNAMICS;

MOLECULAR DYNAMICS;

BINDING PROTEIN; CARBOHYDRATE; CARBOHYDRATE BINDING MODULE; CARBOHYDRATE BINDING MODULE 4 2; LIGAND; UNCLASSIFIED DRUG; X 2 MODULE; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; HYDROPHOBICITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ENGINEERING; RHODOTHERMUS; SITE DIRECTED MUTAGENESIS;

AMIDES; AMINO ACID SEQUENCE; BETA-GLUCANS; ENDO-1,4-BETA XYLANASES; LIGANDS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RHODOTHERMUS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SOLUBILITY; STRUCTURAL HOMOLOGY, PROTEIN; THERMODYNAMICS; XYLANS;

RHODOTHERMUS MARINUS;

EID: 34548178992     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070128     Document Type: Article

References (21)

1. Bolam, D. N., Ciruela, A., McQueen-Mason, S., Simpson, P., Williamson, M. P., Rixon, J. E., Boraston, A., Hazlewood, G. P. and Gilbert, H. J. (1998) Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity. Biochem. J. 331, 775-781
2. Gilkes, N. R., Warren, R. A., Miller, Jr, R. C. and Kilburn, D. G. (1988) Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. J. Biol. Chem. 263, 10401-10407
3. Boraston, A. B., Bolam, D. N., Gilbert, H. J. and Davies, G. J. (2004) Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem. J. 382, 769-781
4. Boraston, A. B., McLean, B. W., Kormos, J. M., Alam, M., Gilkes, N. R., Haynes, C. A., Tomme, P., Kilburn, D. G. and Warren, R. A. J. (1999) Carbohydrate binding modules: diversity of structure and function. In Recent Advances in Carbohydrate Engineering (Gilbert, H. J., Davies, G. J., Svensson, B. and Henrissat, B., eds.), pp. 202-211, Royal Society of Chemistry, Cambridge, U.K.
5. Pell, G., Williamson, M. P., Walters, C., Du, H., Gilbert, H. J. and Bolam, D. N. (2003) Importance of hydrophobic and polar residues in ligand binding in the family 15 carbohydrate-binding module from Cellovibrio japonicus Xyn10C. Biochemistry 42, 9316-9323
6. Xie, H., Bolam, D. N., Nagy, T., Szabo, L., Cooper, A., Simpson, P. J., Lakey, J. H., Williamson, M. P. and Gilbert, H. J. (2001) Role of hydrogen bonding in the interaction between a xylan binding module and xylan. Biochemistry 40, 5700-5707
7. Abou Hachem, M., Nordberg Karlsson, E., Bartonek-Roxa, E., Raghothama, S., Simpson, P. J., Gilbert, H. J., Williamson, M. P. and Holst, O. (2000) Carbohydrate-binding modules from a thermostable Rhodothermus marinus xylanase: cloning, expression and binding studies. Biochem. J. 345, 53-60
8. Czjzek, M., Bolam, D. N., Mosbah, A., Allouch, J., Fontes, C. M., Ferreira, L. M., Bornet, O., Zamboni, V., Darbon, H., Smith, N. L. et al. (2001) The location of the ligand-binding site of carbohydrate-binding modules that have evolved from a common sequence is not conserved. J. Biol. Chem. 276, 48580-48587
9. Szabo, L., Jamal, S., Xie, H., Charnock, S. J., Bolam, D. N., Gilbert, H. J. and Davies, G. J. (2001) Structure of a family 15 carbohydrate-binding module in complex with xylopentaose. Evidence that xylan binds in an approximate 3-fold helical conformation. J. Biol. Chem. 276, 49061-49065
10. Charnock, S. J., Bolam, D. N., Turkenburg, J. P., Gilbert, H. J., Ferreira, L. M., Davies, G. J. and Fontes, C. M. (2000) The X6 'thermostabilizing' domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain. Biochemistry 39, 5013-5021
11. Cicortas Gunnarsson, L., Zhou, Q., Montanier, C., Nordberg Karlsson, E., Brumer, III, H. and Ohlin, M. (2006) Engineered xyloglucan specificity in a carbohydrate-binding module. Glycobiology 16, 1171-1180
12. Simpson, P. J., Jamieson, S. J., Abou-Hachem, M., Nordberg Karlsson, E., Gilbert, H. J., Holst, O. and Williamson, M. P. (2002) The solution structure of the CBM4-2 carbohydrate binding module from a thermostable Rhodothermus marinus xylanase. Biochemistry 41, 5712-5719
13. Cicortas Gunnarsson, L., Nordberg Karlsson, E., Albrekt, A. S., Andersson, M., Holst, O. and Ohlin, M. (2004) A carbohydrate binding module as a diversity-carrying scaffold. Protein Eng. Des. Sel. 17, 213-221
14. Cicortas Gunnarsson, L., Dexlin, L., Nordberg Karlsson, E., Holst, O. and Ohlin, M. (2006) Evolution of a carbohydrate binding module into a protein-specific binder. Biomol. Eng. 23, 111-117
15. Gill, S. C. and von Hippel, P. H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182, 319-326
16. Flint, J., Nurizzo, D., Harding, S. E., Longman, E., Davies, G. J., Gilbert, H. J. and Bolam, D. N. (2004) Ligand-mediated dimerization of a carbohydrate-binding molecule reveals a novel mechanism for protein-carbohydrate recognition. J. Mol. Biol. 337, 417-426
17. Charlton, A. J., Baxter, N. J., Khan, M. L., Moir, A. J., Haslam, E., Davies, A. P. and Williamson, M. P. (2002) Polyphenol/peptide binding and precipitation. J. Agric. Food Chem. 50, 1593-1601
18. Nagy, T., Simpson, P., Williamson, M. P., Hazlewood, G. P., Gilbert, H. J. and Orosz, L. (1998) All three surface tryptophans in Type IIa cellulose binding domains play a pivotal role in binding both soluble and insoluble ligands. FEBS Lett. 429, 312-316
19. Ponyi, T., Szabo, L., Nagy, T., Orosz, L., Simpson, P. J., Williamson, M. P. and Gilbert, H. J. (2000) Trp22, Trp24, and Tyr8 play a pivotal role in the binding of the family 10 cellulose-binding module from Pseudomonas xylanase A to insoluble ligands. Biochemistry 39, 985-991
20. Xie, H., Gilbert, H. J., Charnock, S. J., Davies, G. J., Williamson, M. P., Simpson, P. J., Raghothama, S., Fontes, C. M., Dias, F. M., Ferreira, L. M. and Bolam, D. N. (2001) Clostridium thermoceilum Xyn10B carbohydrate-binding module 22-2: the role of conserved amino acids in ligand binding. Biochemistry 40, 9167-9176
21. Sunna, A., Gibbs, M. D. and Bergquist, P. L. (2001) Identification of novel β-mannan-and β-glucan-binding modules: evidence for a superfamily of carbohydrate-binding modules. Biochem. J. 356, 791-798