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Volumn 29, Issue 10, 2007, Pages 1557-1560

A new 10-min ligation method using a modified buffer system with a very low amount of T4 DNA ligase: The "Coffee Break Ligation" technique

Author keywords

Ligation; Polyethylene glycol; T4 DNA ligase

Indexed keywords

COST EFFECTIVENESS; MOLECULAR BIOLOGY; POLYETHYLENE GLYCOLS;

EID: 34548142273     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9429-z     Document Type: Article
Times cited : (6)

References (9)
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    • Hayashi, K.1    Nakazawa, M.2    Ishizaki, Y.3    Obayashi, A.4
  • 2
    • 0022432558 scopus 로고
    • Stimulation of intermolecular ligation with E. coli DNA ligase by high concentrations of monovalent cations in polyethylene glycol solutions
    • Hayashi K, Nakazawa M, Ishizaki Y, Hiraoka N, Obayashi A (1985b) Stimulation of intermolecular ligation with E. coli DNA ligase by high concentrations of monovalent cations in polyethylene glycol solutions. Nucleic Acids Res 13:7979-7992
    • (1985) Nucleic Acids Res , vol.13 , pp. 7979-7992
    • Hayashi, K.1    Nakazawa, M.2    Ishizaki, Y.3    Hiraoka, N.4    Obayashi, A.5
  • 3
    • 0023046280 scopus 로고
    • Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol
    • Hayashi K, Nakazawa M, Ishizaki Y, Hiraoka N, Obayashi A (1986) Regulation of inter- and intramolecular ligation with T4 DNA ligase in the presence of polyethylene glycol. Nucleic Acids Res 14:7617-7631
    • (1986) Nucleic Acids Res , vol.14 , pp. 7617-7631
    • Hayashi, K.1    Nakazawa, M.2    Ishizaki, Y.3    Hiraoka, N.4    Obayashi, A.5
  • 4
    • 0025675856 scopus 로고
    • High efficiency transformation of Escherichia coli with plasmids
    • Inoue H, Nojima H, Okayama H (1990) High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28
    • (1990) Gene , vol.96 , pp. 23-28
    • Inoue, H.1    Nojima, H.2    Okayama, H.3
  • 5
    • 0021112785 scopus 로고
    • Polymer-stimulated ligation: Enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions
    • Pheiffer BH, Zimmerman SB (1983) Polymer-stimulated ligation: enhanced blunt- or cohesive-end ligation of DNA or deoxyribooligonucleotides by T4 DNA ligase in polymer solutions. Nucleic Acids Res 11:7853-7871
    • (1983) Nucleic Acids Res , vol.11 , pp. 7853-7871
    • Pheiffer, B.H.1    Zimmerman, S.B.2
  • 6
    • 0022431950 scopus 로고
    • Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase
    • Rusche JR, Howard-Flanders P (1985) Hexamine cobalt chloride promotes intermolecular ligation of blunt end DNA fragments by T4 DNA ligase. Nucleic Acids Res 13:1997-2008
    • (1985) Nucleic Acids Res , vol.13 , pp. 1997-2008
    • Rusche, J.R.1    Howard-Flanders, P.2
  • 7
    • 0003252192 scopus 로고    scopus 로고
    • Plasmids and Their Usefulness in Molecular Cloning
    • 3 Cold Spring Harbor Laboratory Press New York
    • Sambrook J, Russell DW (2001) Plasmids and Their Usefulness in Molecular Cloning. In: Sambrook J, Russell DW (eds) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York, pp 1.1-1.170
    • (2001) Molecular Cloning: A Laboratory Manual , pp. 11-1170
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  • 8
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    • Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli
    • Zimmerman SB, Pheiffer BH (1983) Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli. Proc Natl Acad Sci USA 80:5852-5856
    • (1983) Proc Natl Acad Sci USA , vol.80 , pp. 5852-5856
    • Zimmerman, S.B.1    Pheiffer, B.H.2
  • 9
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    • Macromolecular crowding accelerates the cohesion of DNA fragments with complementary termini
    • Zimmerman SB, Harrison B (1985) Macromolecular crowding accelerates the cohesion of DNA fragments with complementary termini. Nucleic Acids Res 13:2241-2249
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.