Quantitative Determination of Lipase Activity by Liquid Chromatography-Mass Spectrometry

Journal of the American Society for Mass Spectrometry

Volumn 18, Issue 9, 2007, Pages 1579-1581

  Hao, Gang ^a   Yang, Lan ^a   Mazsaroff, Istvan ^a   Lin, Melanie ^a  

^a Altus Pharmaceuticals Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; OLEIC ACID; PH EFFECTS;

IN VITRO LIPASE ASSAY; LIPASE ACTIVITY ASSAY; TRIOLEIN;

ENZYME ACTIVITY;

CARBON 13; FATTY ACID; OLEIC ACID; TRIACYLGLYCEROL LIPASE; TRIOLEIN;

ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME RELEASE; FATTY ACID ANALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PH; PROTEIN ISOLATION; QUANTITATIVE ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; STANDARD;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; LIPASE; OLEIC ACID; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTRATE SPECIFICITY;

EID: 34548124524     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2007.05.019     Document Type: Article

References (19)

1. Dakin H.D. The hydrolysis of optically inactive esters by means of enzymes: Part I. The action of lipase upon esters of mandelic acid. The resolution of inactive mandelic acid. J. Physiol 30 (1903) 253-263
2. Mu H., and Porsgaard T. The metabolism of structured triacylglycerols. Prog. Lipid Res 44 (2005) 430-448
3. Spiegel S., Foster D., and Kolesnick R. Signal transduction through lipid second messengers. Curr. Opin. Cell. Biol 8 (1996) 159-167
4. Paradis M.-E., Badellino K.O., Rader D.J., Deshaies Y., Couture P., Archer W.R., Bergeron N., and Lamarche B. Endothelial lipase is associated with inflammation in humans. J. Lipid Res 47 (2006) 2808-2813
5. Layer P., Keller J., and Lankisch P.G. Pancreatic enzyme replacement therapy. Curr. Gastroenterol. Rep 3 (2001) 101-108
6. Carroccio A., Iacono G., and Montalto G. Pancreatic enzyme therapy in childhood celiac disease. A double-blind prospective randomized study. Dig. Dis. Sci 40 (1995) 2555-2560
7. Brady M.S., Garson J.L., Krug S.K., Kaul A., Richard K.A., Caffrey H.H., Fineberg N., Balistreri W.F., and Stevens J.C. An enteric-coated high-buffered pancrelipase reduces steatorrhea in patients with cystic fibrosis: A prospective, randomized study. J. Am. Diet Assoc 106 (2006) 1181-1186
8. Schmid R.D., and Verger R. Lipases: Interfacial enzymes with attractive applications. Angew. Chem. Int. Ed 37 (1998) 1608-1633
9. Beisson F., Tiss A., Rivière C., and Verger R. Methods for lipase detection and assay: A critical review. Eur. J. Lipid Sci. Technol 102 (2000) 133-153
10. Schmidt M., and Bornscheuer U.T. High-throughput assays for lipases and esterases. Biomol. Eng 22 (2005) 51-66
11. Frazer A.C., and Sammons H.G. The formation of mono- and diglycerides during the hydrolysis of triglyceride by pancreatic lipase. Biochem. J 39 (1945) 122-128
12. Hills M.J., and Mukherjee K.D. Assay for triacylglycerol lipase by a rapid thin-layer chromatographic technique. J. Lipid Res 29 (1988) 1397-1399
13. Briquet-Laugier V., Ben-Zeev O., and Doolittle M.H. Determining lipoprotein lipase and hepatic lipase activity using radiolabeled substrates. Methods Mol. Biol 109 (1999) 81-94
14. Duque M., Graupner M., Stütz H., Wicher I., Zechner R., Paltauf F., and Hermetter A. New fluorogenic triacylglycerol analogs as substrates for the determination and chiral discrimination of lipase activities. J. Lipid Res 37 (1996) 868-876
15. Hendrickson H.S. Fluorescence-based assays of lipases, phospholipases, and other lipolytic enzymes-a review. Anal. Biochem 219 (1994) 1-8
16. Brockman H.L. Triglyceride lipase from porcine pancreas. Methods Enzymol 71 (1981) 619-627
17. Isaac G., Jeannotte R., Esch S.W., and Welti R. New mass-spectrometry-based strategies for lipids. Genet. Eng 28 (2007) 129-157 (NY)
18. Byss M., Triska J., and Elhottova D. GC-MS-MS analysis of bacterial fatty acids in heavily creosote-contaminated soil samples. Anal. Bioanal. Chem (2007) in press
19. Lee S.H., Pettinella C., and Blair I.A. LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Curr. Drug Metab 7 (2006) 929-937