Electron microscopy in situ hybridization: Tracking of DNA and RNA sequences at high resolution

Methods in Molecular Biology

Volumn 369, Issue , 2007, Pages 213-228

  Cmarko, Dušan ^a   Koberna, Karel ^a  

^a NONE

Author keywords

Cryosections; Double and single stranded DNA and RNA; Electron microscopy; In situ hybridization; Post embedding; Preembedding

Indexed keywords

DEOXYRIBONUCLEASE I; DNA; FIXATIVE; PANCREATIC RIBONUCLEASE; PROTEINASE K; RNA; VIRUS RNA;

ANIMAL; ARTICLE; CELL STRAIN COS1; CERCOPITHECUS; COMPARATIVE STUDY; CRYOELECTRON MICROSCOPY; DNA DENATURATION; ELECTRON MICROSCOPY; GENETICS; HELA CELL; HISTOLOGY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; IN SITU HYBRIDIZATION; METHODOLOGY; MICROTOMY; MOLECULAR PROBE; RNA GENE; ULTRASTRUCTURE;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; CRYOELECTRON MICROSCOPY; DEOXYRIBONUCLEASE I; DNA; ENDOPEPTIDASE K; FIXATIVES; GENES, RRNA; HELA CELLS; HIV-1; HUMANS; IN SITU HYBRIDIZATION; MICROSCOPY, ELECTRON; MICROTOMY; MOLECULAR PROBES; NUCLEIC ACID DENATURATION; PLASTIC EMBEDDING; RIBONUCLEASE, PANCREATIC; RNA; RNA, VIRAL;

EID: 34548025588     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-294-6_11     Document Type: Article

References (44)

1. Pardue, M. L. and Gall, J. G. (1969) Molecular hybridization of radioactive DNA to the DNA of cytological preparation. Proc. Natl. Acad. Sci. USA 64, 600-604.
2. John, H. A., Birnstiel, M. L., and Jones, K. W. (1969) RNA-DNA hybrids at the cytological level. Nature (London) 223, 582-587.
3. Jacob, J., Todd, K., Birnstiel, M. L., and Bird, A. (1971) Molecular hybridization of 3H-labelled ribosomal RNA with DNA in ultrathin sections prepared for electron microscopy. Biochim. Biophys. Acta 228, 761-766.
4. Croissant, O., Dauguet, C., Jeanteur, P., and Orth, G. (1972) Application of the molecular hybridization technic in situ for the demonstration with the electron microscope, of vegetative replication of viral DNA in the papillomas induced with Shope vims in cottontail rabbits. CR. Acad. Sci. Hebd. Seances Acad. Sci. D. 274, 614-617.
5. Binder, M., Tourmente, S., Roth, J., Renaud, M., and Gehring, W. J. (1986) In situ hybridization at the electron microscope level: localization of transcripts on ultrathin sections of Lowicryl K4M-embedded tissue using biotinylated probes and protein A-gold complexes. J. Cell. Biol. 102, 1646-1653.
6. McFadden, G. I., Bonig, I., Cornish, E. C., and Clarke, A. E. (1988) A simple fixation and embedding method for use in hybridization histochemistry on plant tissues. Histochem. J. 20, 575-586.
7. Puvion-Dutilleul, F., Pichard, E., Laithier, M., and Puvion, E. ( 1989) Cytochemical study of the localization and organization of parental herpes simplex virus type I DNA during initial infection of the cell. Eur. J. Cell Biol. 50,187-200.
8. Le Guellec, D., Frappart, L., and Willems, R. (1990) Ultrastructural localization of fibronectin mRNA in chick embryo by in situ hybridization using 35 S or biotin labeled cDNA probes. Biol. Cell 70, 159-165.
9. Wachtler, F., Mosgoller, W., and Schwarzacher, H. G. (1990) Electron microscopic in situ hybridization and autoradiography: localization and transcription of rDNA in human lymphocyte nucleoli. Exp. Cell Res. 187, 346-348.
10. Wenderoth, M. P. and Eisenberg, B. R. (1991) Ultrastructural distribution of myosin heavy chain mRNA in cardiac tissue: a comparison of frozen and LR White embedment. J. Histochem. Cytochem. 39, 1025-1033.
11. Puvion-Dutilleul, F. and Puvion, E. (1991) Sites of transcription of adenovirus type 5 genomes in relation to early viral DNA replication in infected HeLa cells. A high resolution in situ hybridization and autoradiographical study. Biol. Cell 71, 135-147.
12. Puvion-Dutilleul, F., Bachellerie, J. P., and Puvion, E. (1991) Nucleolar organization of HeLa cells as studied by in situ hybridization. Chromosoma 100, 395-409.
13. Domes, U., Bartsch, U., Noite, C., Roth, J., and Schachner, M. (1993) Adaptation of a non-radioactive in situ hybridization method to electron microscopy: detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies. Histochemistry 99, 251-262.
14. Olmedilla, A., Testillano, P. S., Vicente, O., Delseny, M., and Risueno, M. C. (1993) Ultrastructural rRNA localization in plant cell nucleoli. RNA/RNA in situ hybridization, autoradiography and cytochemistry.J. Cell Sci. 106, 1333-1346.
15. Mosgoller, W., Schofer, C., Derenzini, M., Steiner, M., Maier, U., and Wachtier, F. (1993) Distribution of DNA in human Sertoli cell nucleoli. J. Histochem. Cytochem. 41, 1487-1493.
16. Morey, A. L., Ferguson, D. J., Leslie, K. O., Taatjes, D. J., and Fleming, K. A. (1993) Intracellular localization of parvovirus B19 nucleic acid at the ultrastructural level by in situ hybridization with digoxigenin-labelled probes. Histochem. J. 25, 421-429.
17. Lin, N. S., Chen, C. C., and Hsu, Y. H. (1993) Post-embedding in situ hybridization for localization of viral nucleic acid in ultra-thin sections. J. Histochem. Cytochem. 41, 1513-1519.
18. Visa, N., Puvion-Dutilleul, F., Harper, F., Bachellerie, J. P., and Puvion, E. (1993) Intranuclear distribution of poly(A) RNA determined by electron microscope in situ hybridization. Exp. Cell Res. 208, 19-34.
19. Visa, N., Puvion-Dutilleul, F., Bachellerie, J. P., and Puvion, E. (1993) Intranuclear distribution of U1 and U2 snRNAs visualized by high resolution in situ hybridization: revelation of a novel compartment containing U1 but not U2 snRNA in HeLa cells. Eur. J. Cell Biol. 60, 308-321.
20. Raška, I., Dundr, M., Koberna, K., Melčák, I., Risueno, M. C., and Torok, I. (1995) Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal. J. Struct. Biol. 114, 1-22.
21. Morey, A. L., Ferguson, D. J., and Fleming, K. A. (1995) Combined immunocytochemistry and non-isotopic in situ hybridization for the ultrastructural investigation of human parvovirus B19 infection. Histochem. J. 27, 46-53.
22. Pierron, G. and Puvion-Dutilleul, F. (1996) Localization of the newly initiated and processed ribosomal primary transcripts during the mitotic cycle in Physarum polycephalum. Exp. Cell Res. 229, 407-420.
23. Melčák, I., Cermanová, S., Jirsová, K., Koberna, K., Malínský, J., and Raška, I. (2000) Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs. Mol. Biol. Cell. 11,497-510.
24. Koberna, K., Malínský, J., Pliss, A., et al. (2002) Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ. J. Cell Biol. 157, 743-748.
25. Cmarko, D., Boe, S. O., Scassellati, C., et al. (2002) Rev inhibition strongly affects intranuclear distribution of human immunodeficiency viras type 1 RNAs. J. Virol. 76, 10473-10484.
26. Vazquez-Nin, G. H., Echeverria, O. M., Ortiz, R., et al. (2003) Fine structural cytochemical analysis of homologous chromosome recognition, alignment, and pairing in Guinea pig spermatogonia and spermatocytes. Biol. Reprod. 69, 1362-1370.
27. Liebe, B., Alsheimer, M., Hoog, C., Benavente, R., and Scherthan, H. (2004) Telomere attachment, meiotic chromosome condensation, pairing, and bouquet stage duration are modified in spermatocytes lacking axial elements. Mol. Biol. Cell. 15, 827-837.
28. Webster, H. F., Lamperth, L., Favilla, J. T., Lemke, G., Tesin, D., and Manuelidis, L. (1987) Use of a biotinylated probe and in situ hybridization for light and electron microscopic localization of Po mRNA in myelin-forming Schwann cells. Histochemistry 86, 441-444.
29. Wolber, R. A., Beals, T. F., and Maassab, H. F. ( 1989) Ultrastractural localization of herpes simplex virus RNA by in situ hybridization, J. Histochem. Cytochem. 37, 97-104.
30. Liposits, Z., Petersen, S. L., and Pauli, W. K. (1991) Amplification of the in situ hybridization signal by silver postintensification: the biotin-dUTP-streptavidinperoxidase diaminobenzidine-silver-gold detection system. Histochemistry 96, 339-342.
31. Le Guellec, D., Trembleau, A., Pechoux, C., Gossard, F., and Morel, G. (1992) Ultrastructural non-radioactive in situ hybridization of GH mRNA in rat pituitary gland: pre-embedding vs ultra-thin frozen sections vs post-embedding. J. Histochem. Cytochem. 40, 979-986.
32. Mitchell, V., Gambiez, A., and Beauvillain, J. C. (1993) Fine-structural localization of proenkephalin mRNAs in the hypothalamic magnocellular dorsal nucleus of the guinea pig: a comparison of radioisotopic and enzymatic in situ hybridization methods at the light- and electron-microscopic levels. Cell Tissue Res. 274, 219-228.
33. Sibon, O. C., Humbel, B. M., DeGraaf, A., Verkleij, A. J., and Cremers, F. F. (1994) Ultrastractural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement. Histochemistry 101, 223-232.
34. Sibon, O. C., Cremers, F. F., Humbel, B. M., Boonstra, J., and Verkleij, A. J. (1995) Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes. Histochem. J. 27, 35-45.
35. Gonzalez-Melendi, P. and Shaw, P. (2002) 3D gold in situ labelling in the EM. Plant J. 29, 237-243.
36. Tang, M., Owens, K., Pietri, R., Zhu, X. R., McVeigh, R., and Ghosh, B. K. (1989) Cloning of the crystalline cell wall protein gene of Bacillus licheniformis NM 105. J. Bacterial. 171, 6637-6648.
37. Morel, G., Chabot, J. G., Gossard, F., and Heisler, S. (1989) Is atrial natriuretic peptide synthesized and internalized by gonadotrophs? Endocrinology 124, 1703-1710.
38. Dirks, R. W., Van Dorp, A. G., Van Minnen, J., Fransen, J. A., Van der Ploeg, M., and Raap, A. K. (1992) Electron microscopic detection of RNA sequences by nonradioactive in situ hybridization in the mollusk Lymnaea stagnalis. J. Histochem. Cytochem. 40, 1647-1657.
39. Yao, C. H., Kitazawa, S., Fujimori, T., and Maeda, S. (1993) In situ hybridization at the electron microscopic level using a bromodeoxyuridine labeled DNA probe. Biotech. Histochem. 68, 169-174.
40. Mandry, P., Murray, B. A., Rieke, L., Becke, H., and Hofler, H. (1993) Postembedding ultrastructural in situ hybridization on ultrathin cryosections and LR white resin sections. Ultrastruct. Pathol. 17, 185-194.
41. Li, K., Smagula, C. S., Parsons, W. J., et al. (1994) Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis. J. Cell Biol. 124, 871-882.
42. Takizawa, T. and Robinson, J. M. (1994) Use of 1.4-nm immunogold particles for immunocytochemistry on ultra-thin cryosections. J. Histochem. Cytochem. 42, 1615-1623.
43. Zalokar, M. and Erk, I. (1977) Phase-partition fixation and staining ofDrosophila eggs. Stain. Technol. 52, 89-95.
44. Le Guellec, D. (1998) Ultrastructural in situ hybridization: a review of technical aspects. Biol. Cell. 90, 297-306.