Isolation, purification and expansion of myelination-competent, neonatal mouse Schwann cells

European Journal of Neuroscience

Volumn 26, Issue 4, 2007, Pages 953-964

  Honkanen, Henrika ^a   Lahti, Outi ^a   Nissinen, Marja ^a   Myllylä, Riina M ^a   Kangas, Salla ^a   Päiväläinen, Satu ^a   Alanne, Maria H ^b   Peltonen, Sirkku ^b   Peltonen, Juha ^b   Heape, Anthony M ^a  

^a UNIVERSITY OF OULU   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

Culture; Glial cell; Growth; Myelin; Peripheral nervous system

Indexed keywords

MYELIN; MYELIN ASSOCIATED GLYCOPROTEIN; MYELIN BASIC PROTEIN; NEU DIFFERENTIATION FACTOR;

ANIMAL EXPERIMENT; ARTICLE; CELL GROWTH; CELL ISOLATION; CELL PROLIFERATION; CELL SPECIFICITY; CONTROLLED STUDY; CROSS BREEDING; GENE EXPRESSION; GENOTYPE; MOUSE; MYELINATION; NERVE CELL; NERVE CELL CULTURE; NERVE GROWTH; NEWBORN; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PURIFICATION; SCHWANN CELL; SCIATIC NERVE; TISSUE EXPANSION; WILD TYPE;

ANIMALS; ANIMALS, NEWBORN; ANTIBODIES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SURVIVAL; COCULTURE TECHNIQUES; COLLAGEN; CULTURE MEDIA; FLUORESCENT ANTIBODY TECHNIQUE; GANGLIA, SPINAL; LAMININ; MICE; MICROSCOPY, PHASE-CONTRAST; MYELIN SHEATH; NERVE TISSUE; NEURONS; POLYLYSINE; SCHWANN CELLS;

EID: 34547969740     PISSN: 0953816X     EISSN: 14609568     Source Type: Journal    
DOI: 10.1111/j.1460-9568.2007.05726.x     Document Type: Article

References (31)

1. Baron-Van Evercooren, A., Kleinman, H.K., Seppa, H.E., Rentier, B. Dubois-Dalcq, M. (1982) Fibronectin promotes rat Schwann cell growth and motility. J. Cell Biol., 93, 211 216.
2. Brockes, J.P. Raff, M.C. (1979) Studies on cultured rat Schwann cells. II. Comparison with a rat Schwann cell line. In Vitro, 15, 772 778.
3. Brockes, J.P., Fields, K.L. Raff, M.C. (1979) Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve. Brain Res., 165, 105 118.
4. Casella, G.T., Bunge, R.P. Wood, P.M. (1996) Improved method for harvesting human Schwann cells from mature peripheral nerve and expansion in vitro. Glia, 17, 327 338.
5. Corfas, G., Velardez, M.O., Ko, C.P., Ratner, N. Peles, E. (2004) Mechanisms and roles of axon-Schwann cell interactions. J. Neurosci., 24, 9250 9260.
6. Dong, Z., Sinanan, A., Parkinson, D., Parmantier, E., Mirsky, R. Jessen, K.R. (1999) Schwann cell development in embryonic mouse nerves. J. Neurosci. Res., 56, 334 348.
7. Garbay, B., Fournier, M., Sallafranque, M.L., Muller, S., Boiron, F., Heape, A., Cassagne, C. Bonnet, J. (1988) Po, MBP, histone, and DNA levels in sciatic nerve. Postnatal accumulation studies in normal and trembler mice. Neurochem. Pathol., 8, 91 107.
8. Hyden, H. McEwen, B. (1966) A glial protein specific for the nervous system. Proc. Natl Acad. Sci. U.S.A., 55, 354 358.
9. Jessen, K.R., Thorpe, R. Mirsky, R. (1984) Molecular identity, distribution and heterogeneity of glial fibrillary acidic protein: An immunoblotting and immunohistochemical study of Schwann cells, satellite cells, enteric glia and astrocytes. J. Neurocytol., 13, 187 200.
10. Jessen, K.R., Morgan, L., Stewart, H.J. Mirsky, R. (1990) Three markers of adult non-myelin-forming Schwann cells, 217c(ran-1), A5E3 and GFAP: Development and regulation by neuron- Schwann cell interactions. Development, 109, 91 103.
11. Kim, H.A., Rosenbaum, T., Marchionni, M.A., Ratner, N. De Clue, J.E. (1995) Schwann cells from neurofibromin-deficient mice exhibit activation of p21ras, inhibition of cell proliferation and morphological changes. Oncogene, 11, 325 335.
12. Kim, H.A., Ling, B. Ratner, N. (1997) Nf1-deficient mouse Schwann cells are angiogenic and invasive and can be induced to hyperproliferate: Reversion of some phenotypes by an inhibitor of farnesyl protein transferase. Mol. Cell. Biol., 17, 862 872.
13. Kleitman, N., Wood, P.M. Bunge, R.P. (1997) Tissue culture methods for the study of myelination. In Cowan, W.M., Jessell, T.M. Zipursky, S.L. (Eds), Molecular and Cellular Approaches to Neural Development. Oxford University Press, New York/Oxford, pp. 337 377.
14. Lepore, G., Zedda, M., Gadau, S. Farina, V. (2003) Purified Schwann cell primary cultures from mouse and rat sciatic nerve. Arch. Ital. Biol., 141, 207 210.
15. Liu, N., Varma, S., Shooter, E.M. Tolwani, R.J. (2005) Enhancement of Schwann cell myelin formation by K252a in the trembler-J mouse dorsal root ganglion explant culture. J. Neurosci. Res., 79, 310 317.
16. Manent, J., Oguievetskaia, K., Bayer, J., Ratner, N. Giovannini, M. (2003) Magnetic cell sorting for enriching Schwann cells from adult mouse peripheral nerves. J. Neurosci. Meth., 123, 167 173.
17. Nicholson, S.M., Gomes, D., de Nechaud, B. Bruzzone, R. (2001) Altered gene expression in Schwann cells of connexin32 knockout animals. J. Neurosci. Res., 66, 23 36.
18. Nobbio, L., Gherardi, G., Vigo, T., Passalacqua, M., Melloni, E., Abbruzzese, M., Mancardi, G., Nave, K.A. Schenone, A. (2006) Axonal damage and demyelination in long-term dorsal root ganglia cultures from a rat model of Charcot-Marie-Tooth type 1A disease. Eur. J. Neurosci., 23, 1445 1452.
19. Pannunzio, M.E., Jou, I.M., Long, A., Wind, T.C., Beck, G. Balian, G. (2005) A new method of selecting Schwann cells from adult mouse sciatic nerve. J. Neurosci. Meth., 149, 74 81.
20. Porter, S., Clark, M.B., Glaser, L. Bunge, R.P. (1986) Schwann cells stimulated to proliferate in the absence of neurons retain full functional capability. J. Neurosci., 6, 3070 3078.
21. Raff, M.C., Hornby-Smith, A. Brockes, J.P. (1978) Cyclic AMP as a mitogenic signal for cultured rat Schwann cells. Nature, 273, 672 673.
22. Ratner, N., Hong, D.M., Lieberman, M.A., Bunge, R.P. Glaser, L. (1988) The neuronal cell-surface molecule mitogenic for Schwann cells is a heparin-binding protein. Proc. Natl Acad. Sci. U.S.A., 85, 6992 6996.
23. Salzer, J.L. Bunge, R.P. (1980) Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, wallerian degeneration, and direct injury. J. Cell Biol., 84, 739 752.
24. Salzer, J.L., Williams, A.K., Glaser, L. Bunge, R.P. (1980) Studies of Schwann cell proliferation. II. Characterization of the stimulation and specificity of the response to a neurite membrane fraction. J. Cell Biol., 84, 753 766.
25. Seilheimer, B. Schachner, M. (1987) Regulation of neural cell adhesion molecule expression on cultured mouse Schwann cells by nerve growth factor. EMBO J., 6, 1611 1616.
26. Seilheimer, B., Persohn, E. Schachner, M. (1989) Antibodies to the L1 adhesion molecule inhibit Schwann cell ensheathment of neurons in vitro. J. Cell Biol., 109, 3095 3103.
27. Sereda, M., Griffiths, I., Puhlhofer, A., Stewart, H., Rossner, M.J., Zimmerman, F., Magyar, J.P., Schneider, A., Hund, E., Meinck, H.M., Suter, U. Nave, K.A. (1996) A transgenic rat model of Charcot-Marie-Tooth disease. Neuron, 16, 1049 1060.
28. Verdu, E., Rodriguez, F.J., Gudino-Cabrera, G., Nieto-Sampedro, M. Navarro, X. (2000) Expansion of adult Schwann cells from mouse predegenerated peripheral nerves. J. Neurosci. Meth., 99, 111 117.
29. White, F.V., Ceccarini, C., Georgieff, I., Matthieu, J.-M. Costantino-Ceccarini, E. (1983) Growth properties and biochemical characterization of mouse Schwann cells cultured in vitro. Exp. Cell Res., 148, 183 194.
30. Wood, P.M. (1976) Separation of functional Schwann cells and neurons from normal peripheral nerve tissue. Brain Res., 115, 361 375.
31. Yamada, H., Komiyama, A. Suzuki, K. (1995) Schwann cell responses to forskolin and cyclic AMP analogues: Comparative study of mouse and rat Schwann cells. Brain Res., 681, 97 104.