Testing mounting media to eliminate background noise in confocal microscope 3-D images of insect genitalia

Scanning

Volumn 29, Issue 4, 2007, Pages 177-184

  Schawaroch, Valerie ^a,b   Li, Sarah C ^a  

^a CITY UNIVERSITY OF NEW YORK   (United States)

^b DIVISION OF INVERTEBRATE ZOOLOGY   (United States)

Author keywords

Background noise; Confocal microscopy; Insect genitalia; Mounting media; Three dimensional reconstruction

Indexed keywords

ANIMALS; BIOLOGICAL ORGANS; CONFOCAL MICROSCOPY; IMAGE ANALYSIS;

BACKGROUND NOISE; INSECT GENITALIA; MOUNTING MEDIA; THREE-DIMENSIONAL RECONSTRUCTION;

THREE DIMENSIONAL COMPUTER GRAPHICS;

AGAR; AGAROSE; CARBOHYDRATE; GELATIN; GLYCEROL; LIPID; PECTIN; PETROLATUM; PROTEIN;

ANIMAL TISSUE; ARTICLE; CONFOCAL LASER MICROSCOPY; CONFOCAL MICROSCOPY; CUTICLE; FLUORESCENCE; GENITAL SYSTEM; IMAGE QUALITY; INSECT; NOISE; NONHUMAN; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

EID: 34547940485     PISSN: 01610457     EISSN: None     Source Type: Journal    
DOI: 10.1002/sca.20061     Document Type: Article

References (9)

1. Galassi DMP: The genus Pseudectinosoma Kunz, 1935: an update, and description of Pseudectinosomakunzi sp. n. from Italy (crustacea: copepoda: ectinosomatidae). Arch Hydrobiol 139, 277 (1997a).
2. Galassi DMP: Little known harpacticoid copepods from Italy, and description of Parastenocaris crenobia n. sp. (Copepoda: Harpacticoida). Crustaceana 70, 694 (1997b).
3. Galassi DMP, De Laurentis P, Giammatteo M: Integumental morphology in copepods: Assessment by confocal laser scanning microscopy (CLSM). Fragm Entomol (Roma) 30, 79 (1998).
4. Klaus AV, Kulasekera VL, Schawaroch V: Three-dimensional visualization of insect morphology using confocal laser scanning microscopy. J Microsc 212(2), 107 (2003).
5. Klaus AV, Schawaroch V: Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta). Integr Comp Biol 46(2), 207 (2006).
6. Paddock SW: An introduction to confocal imaging. In Confocal Microscopy: Methods and Protocols, (Ed. Paddock SW), Humana Press: Totowa, 1 (1999).
7. Schawaroch V, Grimaldi D, Klaus AV: Focusing on morphology: Applications and implications of confocal laser scanning microscopy (Diptheria: Campichoetidae, Camillidae, Drosophilidae). Proc Entomol Soc Wash 107(2), 323 (2005).
8. Schnell SA, Staines WA, Wessendorf MW: Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J Histochem Cytochem 47(6), 719 (1999).
9. Zill S, Frazier SF, Neff D, Quimby L, Carney M, et al.: Three-dimensional graphic reconstruction of the insect exoskeleton through confocal imaging of endogenous fluorescence. Microsc Res Tech 48, 367 (2000).