Real-time PCR for the detection of Dientamoeba fragilis in fecal samples

Molecular and Cellular Probes

Volumn 21, Issue 5-6, 2007, Pages 400-404

  Verweij, Jaco J ^a   Mulder, Bert ^b   Poell, Bregje ^b   van Middelkoop, Dorien ^b   Brienen, Eric A T ^a   van Lieshout, Lisette ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Laboratory of Medical Microbiology Twente Achterhoek   (Netherlands)

Author keywords

Diarrhea; Dientamoeba fragilis; Real time PCR

Indexed keywords

RIBOSOME RNA;

ARTICLE; CONTROLLED STUDY; DIENTAMOEBA; FECES ANALYSIS; GENETIC ANALYSIS; MICROORGANISM DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN TARGETING; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; DIENTAMOEBA; DNA, PROTOZOAN; FECES; HUMANS; POLYMERASE CHAIN REACTION;

DIENTAMOEBA FRAGILIS;

EID: 34547913799     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2007.05.006     Document Type: Article

References (21)

1. Jepps M.W., and Dobell C. Dientamoeba fragilis n.g., n. sp., new intestinal amoeba from man. Parasitology 10 (1918) 352-367
2. Camp R.R., Mattern C.F.T., and Honigber B.M. Study of Dientamoeba fragilis Jepps and Dobell-1: electron-microscopic observations of binucleate stages; 2: taxonomic position and revision of genus. J Protozool 21 (1974) 69-82
3. Silberman J.D., Clark C.G., and Sogin M.L. Dientamoeba fragilis shares a recent common evolutionary history with the trichomonads. Mol Biochem Parasitol 76 (1996) 311-314
4. Johnson E.H., Windsor J.J., and Clark C.G. Emerging from obscurity: biological, clinical, and diagnostic aspects of Dientamoeba fragilis. Clin Microbiol Rev 17 (2004) 553-570
5. Stark D.J., Beebe N., Marriott D., Ellis J.T., and Harkness J. Dientamoebiasis: clinical importance and recent advances. Trends Parasitol 22 (2006) 92-96
6. Farthing M.J. Treatment options for the eradication of intestinal protozoa. Nat Clin Pract Gastroenterol Hepatol 3 (2006) 436-445
7. Okhuysen P.C. Traveler's diarrhea due to intestinal protozoa. Clin Infect Dis 33 (2001) 110-114
8. Gendrel D., Treluyer J.M., and Richard-Lenoble D. Parasitic diarrhea in normal and malnourished children. Fund Clin Pharmacol 17 (2003) 189-197
9. van Gool T., Weijts R., Lommerse E., and Mank T.G. Triple faeces test: an effective tool for detection of intestinal parasites in routine clinical practice. Eur J Clin Microbiol Infect Dis 22 (2003) 284-290
10. Verweij J.J., Pit D.S.S., Van Lieshout L., Baeta S.M., Dery G.D., Gasser R.B., et al. Determining the prevalence of Oesophagostomum bifurcum and Necator americanus infections using specific PCR amplification of DNA from faecal samples. Trop Med Int Health 6 (2001) 726-731
11. Subrungruang I., Mungthin M., Chavalitshewinkoon-Petmitr P., Rangsin R., Naaglor T., and Leelayoova S. Evaluation of DNA extraction and PCR methods for detection of Enterocytozoon bienuesi in stool specimens. J Clin Microbiol 42 (2004) 3490-3494
12. Klein D. Quantification using real-time PCR technology: applications and limitations. Trends Mol Med 8 (2002) 257-260
13. Johnson J.A., and Clark C.G. Cryptic genetic diversity in Dientamoeba fragilis. J Clin Microbiol 38 (2000) 4653-4654
14. Peek R., Reedeker F.R., and van Gool T. Direct amplification and genotyping of Dientamoeba fragilis from human stool specimens. J Clin Microbiol 42 (2004) 631-635
15. Stark D., Beebe N., Marriott D., Ellis J., and Harkness J. Detection of Dientamoeba fragilis in fresh stool specimens using PCR. Int J Parasitol 35 (2005) 57-62
16. Stark D., Beebe N., Marriott D., Ellis J., and Harkness J. Evaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens. J Clin Microbiol 44 (2006) 232-235
17. Verweij J.J., Ten Hove R., Brienen E.A., and Van Lieshout L. Multiplex detection of Enterocytozoon bieneusi and Encephalitozoon spp. in fecal samples using real-time PCR. Diagn Microbiol Infect Dis 57 (2007) 163-167
18. Mank T.G., Zaat J.O., Blotkamp J., and Polderman A.M. Comparison of fresh versus sodium acetate acetic acid formalin preserved stool specimens for diagnosis of intestinal protozoal infections. Eur J Clin Microbiol Infect Dis 14 (1995) 1076-1081
19. Niesters H.G. Clinical virology in real time. J Clin Virol 25 Suppl. 3 (2002) 3-12
20. Verweij J.J., Blange R.A., Templeton K., Schinkel J., Brienen E.A.T., van Rooyen M.A.A., et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J Clin Microbiol 42 (2004) 1220-1223
21. Monteiro L., Bonnemaison D., Vekris A., Petry K.G., Bonnet J., Vidal R., et al. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 35 (1997) 995-998