Development of DNA extraction and PCR amplification protocols for detection of Mycoplasma bovis directly from milk samples

Veterinary Research Communications

Volumn 31, Issue SUPPL. 1, 2007, Pages 225-227

  Cremonesi, P ^a   Vimercati, C ^a   Pisoni, G ^a   Perez, G ^a   Ribera, Miranda ^a   Castiglioni, B ^b   Luzzana, M ^a   Ruffo, G ^a   Moroni, Paolo ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b CNR   (Italy)

Author keywords

DNA extraction; Mycoplasma bovis; PCR

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; CONTROLLED STUDY; DNA EXTRACTION; GENE AMPLIFICATION; INTERMETHOD COMPARISON; MILK; MYCOPLASMA BOVIS; NONHUMAN; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; SENSITIVITY AND SPECIFICITY;

ANIMALS; CATTLE; DNA, BACTERIAL; FEMALE; MILK; MYCOPLASMA BOVIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

MYCOPLASMA BOVIS;

EID: 34547852314     PISSN: 01657380     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11259-007-0011-x     Document Type: Article

References (3)

1. Chavez Gonzalez Y.R., Bascunana C.R., Bolske G., Mattsson J.G., Molina C.F. and Johansson K.E., 1995. In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Veterinary Microbiology, 47, 183-190
2. Cremonesi P., Castiglioni B., Malferrari G., Biunno I., Vimercati C., Moroni P., Morandi S. and Luzzana M., 2006. Improved method for rapid DNA extraction of mastitis pathogens directly from milk. Journal of Dairy Science, 89, 163-169
3. Ghadersohi A., Coelen R.J. and Hirst R.G., 1997. Development of a specific DNA probe and PCR for the detection of Mycoplasma bovis. Veterinary Microbiology, 56, 87-98