The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing

PLoS ONE

Volumn 2, Issue 2, 2007, Pages

  Binladen, Jonas ^a   Gilbert, M Thomas P ^a   Bollback, Jonathan P ^a   Panitz, Frank ^b   Bendixen, Christian ^b   Nielsen, Rasmus ^a   Willerslev, Eske ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSINE; DINUCLEOTIDE; DNA; NUCLEOTIDE; THYMINE; MITOCHONDRIAL DNA; PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; DNA SYNTHESIS; GENE ASSIGNMENT; GENE SEQUENCE; GENETIC CODE; HIGH THROUGHPUT SCREENING; HUMAN; NONHUMAN; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; ANIMAL; GENE AMPLIFICATION; GENETICS; INSTRUMENTATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SPECIES DIFFERENCE;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; DNA, MITOCHONDRIAL; GENE AMPLIFICATION; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECIES SPECIFICITY;

EID: 34547834963     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0000197     Document Type: Article

References (7)

1. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376-380.
2. Hofreuter D, Tsai J, Watson RO, Novik V, Altman B, et al. (2006) Unique features of a highly pathogenic Campylobacter jejuni strain. Infect Immun 74: 4694-4707.
3. Thomas RK, Nickerson E, Simons JF, Janne PA, Tengs T, et al. (2006) Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing. Nat Med 12: 852-855.
4. Taylor PG (1996) Reproducibility of ancient DNA sequences from extinct Pleistocene fauna. Mol Biol Evol 13: 283-285.
5. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22: 4673-4680.
6. Gilbert MTP, Binladen J, Miller W, Wiuf C, Willerslev E, et al. Recharacterization of ancient DNA miscoding lesions: insights in the era of sequencing-by-synthesis. Nucleic Acids Res. In press.
7. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J. Mol Biol 215: 403-410.