Use of fused gfp and gus reporters for the recovery of transformed Medicago truncatula somatic embryos without selective pressure

Plant Cell, Tissue and Organ Culture

Volumn 90, Issue 3, 2007, Pages 325-330

  Duque, Ana Sofia ^a   De Sousa Araújo, Susana ^a   Cordeiro, Matilde Ataíde ^a   Santos, Dulce Maria ^a   Fevereiro, Manuel Pedro ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

Author keywords

Agrobacterium mediated transformation; Antibiotic free; Dark embryo induction

Indexed keywords

CELL CULTURE; PROTEINS; SYNTHESIS (CHEMICAL);

AGROBACTERIUM-MEDIATED TRANSFORMATION; ANTIBIOTIC-FREE; DARK EMBRYO INDUCTION;

PLANTS (BOTANY);

AGROBACTERIUM; MEDICAGO TRUNCATULA; RHIZOBIUM;

EID: 34547830278     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-007-9268-6     Document Type: Article

References (17)

1. Araújo SS, Duque AS, Santos DM, Fevereiro P (2004) An efficient transformation method to regenerate a high number of transgenic plants using a new embryogenic line of Medicago truncatula cv. Jemalong. Plant Cell Tiss Organ Cult 78:123-131
2. Barker DG, Bianchi S, Blondon F, Datté Y, Duc G, Essad S, Flament P, Gallusci P, Génier G, Guy P, Muel X, Tourneur J, Dénarié J, Huguet T (1990) Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis. Plant Mol Biol Rep 8:40-49
3. Chabaud M, Larsonneau C, Marmouget C, Huguet T (1996) Transformation of the barrel medic (Medicago truncatula Gaertn.) by Agrobacterium tumefaciens and regeneration via somatic embryogenesis of transgenics plants with the MtENOD12 nodulin promoter fused to the gus reporter gene. Plant Cell Rep 15:305-310
4. Chiu WL, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr Biol 6:325-330
5. Cook DR (1999) Medicago truncatula-a model in the making! Curr Opin Plant Biol 2:301-304
6. Duque AS, Araújo SS, Santos DM, Fevereiro P (2004) Optimisation of a selection scheme using kanamycin to improve transformation of Medicago truncatula cv. Jemalong. Plant Cell Tiss Org Cult 78:227-280
7. Escobar MA, Park J-I, Polito VS, Leslie CA, Uratsu SL, McGranham GH, Dandekar AM (2000) Using GFP as a scorable marker in walnut somatic embryo transformation. Ann Bot 85:831-835
8. Hood EE, Gelvin SB, Melchers LS, Hoekema A (1993) New Agrobacterium helper plasmids for gene transfer to plants. Science 227:1229-1231
9. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387-405
10. Kamaté K, Rodriguez-Llorente ID, Scholte M, Durand P, Ratet P, Kondorosi E, Kondorosi A (2000) Transformation of floral organs with GFP in Medicago truncatula. Plant Cell Rep 19:647-653
11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissues cultures. Physiol Plant 15:473-497
12. Neves LO, Duque SRL, Almeida JS, Fevereiro PS (1999) Repetitive somatic embryogenesis in Medicago truncatula ssp. Narbonensis and M. truncatula Gaertn cv. Jemalong. Plant Cell Rep 18:398-405
13. Neves LO, Tomaz L, Fevereiro MPS (2001) Micropropagation of Medicago truncatula Gaertn. cv. Jemalong and Medicago truncatula ssp. Narbonensis. Plant Cell Tiss Org Cult 67:81-84
14. Quaedvlieg NE, Schlaman HR, Admiraal PC, Wijting SE, Stougaard J, Spaink HP (1998) Fusions between green fluorescent protein and beta-glucuronidase as sensitive and vital bifunctional reporters in plants. Plant Mol Biol 38:861-873
15. Santos D, Fevereiro P (2002) Loss of DNA methylation affects somatic embryogenesis in Medicago truncatula. Plant Cell Tiss Org Cult 70:155-161
16. Walkerpeach CR, Velten J (1994) Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems. In: Gelvin SB, Schilperoort RA (eds) Agrobacterium-mediated gene transfer to plant cells. Plant molecular biology manual, Vol. B1, Kluwer Academic Publishers, London, pp 1-19
17. Zhou X, Carranco R, Vitha S, Hall TC (2005) The dark side of green fluorescent protein. New Phytol 168:313-322