DNA and RNA isolated from tissues processed by microwave-accelerated apparatus MFX-800-3 are suitable for subsequent PCR and Q-RT-PCR amplification

Pathology and Oncology Research

Volumn 13, Issue 2, 2007, Pages 149-152

  Bödör, Csaba ^a   Schmidt, Otto ^b   Csernus, Balázs ^a   Rajnai, Hajnalka ^a   Szende, Béla ^a  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b Meditest Ltd   (Hungary)

Author keywords

MFX 800 3 histoprocessor; Molecular pathology; Paraffin embedding; Real time PCR

Indexed keywords

ABELSON KINASE; BETA GLOBIN; MESSENGER RNA; PARAFFIN;

ARTICLE; COLON CARCINOMA; CONTROLLED STUDY; DNA ISOLATION; GALLBLADDER; GEL ELECTROPHORESIS; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; LIVER; MICROWAVE OVEN; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; TISSUE PREPARATION;

EID: 34547647079     PISSN: 12194956     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02893491     Document Type: Article

References (16)

1. Beillard E, Pallisgaard N, van der Velden VH, et al: Evaluation of candidate control genes for diagnosis and residual disease detection in leukemic patients using 'real-time' quantitative reverse-transcriptase polymerase chain reaction (RQ-PCR) - a Europe against cancer program. Leukemia 17:2474-2486, 2003
2. Benchekroun M, DeGraw J, Gao J, et al: Impact of fixative on recovery of mRNA from paraffin-embedded tissue. Diagn Mol Pathol 13:116-125, 2004
3. Boon ME, Wals-Paap CH, Visinoni FA, et al: The two-step vacuum-microwave method for histoprocessing. Eur J Morphol 33:349-358, 1995
4. Coindre JM, Hostein I, Terrier P, et al: Diagnosis of clear cell sarcoma by real-time reverse transcriptase-polymerase chain reaction analysis of paraffin embedded tissues: clinicopathologic and molecular analysis of 44 patients from the French sarcoma group. Cancer 107:1055-1064, 2006
5. Crocker J: Demystified... Molecular pathology in oncology. Mol Pathol 55:337-347, 2002.
6. Ekuni D, Firth JD, Putnins EE, et al: RNA integrity and in situ RT-PCR in dento-alveolar tissues after microwave accelerated demineralisation. Arch Oral Biol 51:164-169, 2006
7. Gjerdrum LM, Sorensen BS, Kjeldsen E, et al: Real-time quantitative PCR of microdissected paraffin-embedded breast carcinoma: an alternative method for HER-2/neu analysis. J Mol Diagn 6:42-51, 2004
8. Hsu HC, Peng SY, Shun CT, et al: High quality of DNA retrieved for Southern blot hybridization from microwave-fixed, paraffin-embedded liver tissues. J Virol Methods 31:251-261, 1991
9. Kok LP, Visser PE, Boon ME, et al: Histoprocessing with the microwave oven: an update. Histochem J 20:323-328, 1988
10. Kovacs L, Szende B, Elek G, et al: Working experience with a new vacuum-accelerated microwave histoprocessor. J Pathol 180:106-110, 1996
11. Lou YK, Qin H, Molodysky E, et al: Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection. J Virol Methods 44:77-81, 1993
12. Man YG, Burgar A: An antigen unmasking protocol that satisfies both immunohistochemistry and subsequent PCR amplification. Pathol Res Pract 199:815-825, 2003
13. Mies C: Molecular biological analysis of paraffin-embedded tissues. Hum Pathol 25:555-560, 1994
14. Miller SA, Dykes DD, Polesky HF, et al: A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215, 1988
15. Ryan JL, Fan H, Glaser SL, et al: Epstein-Barr virus quantitation by real-time PCR targeting multiple gene segments: a novel approach to screen for the virus in paraffin-embedded tissue and plasma. J Mol Diagn 6:378-385, 2004
16. Vincek V, Nassiri M, Nadji M, et al: A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest 83:1427-1435, 2003