Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae

Journal of Microbiological Methods

Volumn 70, Issue 2, 2007, Pages 384-386

  Vilei, Edy M ^a   Bonvin Klotz, Laetitia ^a   Zimmermann, Liza ^a   Ryser Degiorgis, Marie Pierre ^b   Giacometti, Marco ^c   Frey, Joachim ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b VETSUISSE FACULTY UNIVERSITY OF ZURICH   (Switzerland)

^c Wildvet Projects   (Switzerland)

Author keywords

Conjunctival swabs; Infectious keratoconjunctivitis (IKC); Lipoprotein LppS; Mycoplasma conjunctivae; TaqMan 5 nuclease real time PCR

Indexed keywords

ARTICLE; CAPRINAE; DOMESTIC ANIMAL; HYDROLYSIS; KERATOCONJUNCTIVITIS; MYCOPLASMA CONJUNCTIVAE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHEEP; VALIDATION PROCESS;

ANIMALS; ANIMALS, DOMESTIC; ANIMALS, WILD; GOAT DISEASES; GOATS; KERATOCONJUNCTIVITIS, INFECTIOUS; MYCOPLASMA CONJUNCTIVAE; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION;

ANIMALIA; CAPRINAE; MYCOPLASMA CONJUNCTIVAE;

EID: 34547566273     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.05.007     Document Type: Article

References (13)

1. Akerstedt J., and Hofshagen M. Bacteriological investigation of infectious keratoconjunctivitis in Norwegian sheep. Acta Vet. Scand. 45 (2004) 19-26
2. Belloy L., Giacometti M., Abdo E.-M., Nicolet J., Krawinkler M., Janovsky M., Bruderer U., and Frey J. Detection of specific Mycoplasma conjunctivae antibodies in the sera of sheep with infectious keratoconjunctivitis. Vet. Res. 32 (2001) 155-164
3. Belloy L., Janovsky M., Vilei E.M., Pilo P., Giacometti M., and Frey J. Molecular epidemiology of Mycoplasma conjunctivae in caprinae: transmission across species in natural outbreaks. Appl. Environ. Microbiol. 69 (2003) 1913-1919
4. Belloy L., Vilei E.M., Giacometti M., and Frey J. Characterization of LppS, an adhesin of Mycoplasma conjunctivae. Microbiology 149 (2003) 185-193
5. Degiorgis M.-P., Abdo E.-M., Nicolet J., Frey J., Mayer D., and Giacometti M. Immune responses to Mycoplasma conjunctivae in Alpine ibex, Alpine chamois, and domestic sheep in Switzerland. J. Wildl. Dis. 36 (2000) 265-271
6. Degiorgis M.-P., Frey J., Nicolet J., Abdo E.-M., Fatzer R., Schlatter Y., Reist S., Janovsky M., and Giacometti M. An outbreak of infectious keratoconjunctivitis in Alpine chamois (Rupicapra r. rupicapra) in Simmental-Gruyeres, Switzerland. Schweiz. Arch. Tierh.kd. 142 (2000) 520-527
7. Gaffuri A., Giacometti M., Tranquillo V.M., Magnino S., Cordioli P., and Lanfranchi P. Serosurvey of roe deer, chamois and domestic sheep in the central Italian Alps. J. Wildl. Dis. 42 (2006) 685-690
8. Giacometti M., Nicolet J., Johansson K.E., Naglic T., Degiorgis M.-P., and Frey J. Detection and identification of Mycoplasma conjunctivae in infectious keratoconjunctivitis by PCR based on the 16S rRNA gene. J. Vet. Med., Ser. B 46 (1999) 173-180
9. Giacometti M., Janovsky M., Belloy L., and Frey J. Infectious keratoconjunctivitis of ibex, chamois and other Caprinae. Rev. Sci. Tech. - Off. Int. Épizoot. 21 (2002) 335-345
10. Giacometti M., Janovsky M., Jenny H., Nicolet J., Belloy L., Goldschmidt-Clermont E., and Frey J. Mycoplasma conjunctivae infection is not maintained in alpine chamois in eastern Switzerland. J. Wildl. Dis. 38 (2002) 297-304
11. Janovsky M., Frey J., Nicolet J., Belloy L., Goldschmidt-Clermont E., and Giacometti M. Mycoplasma conjunctivae infection is self-maintained in the Swiss domestic sheep population. Vet. Microbiol. 83 (2001) 11-22
12. Mayer D., Nicolet J., Giacometti M., Schmitt M., Wahli T., and Meier W. Isolation of Mycoplasma conjunctivae from conjunctival swabs of Alpine ibex (Capra ibex ibex) affected with infectious keratoconjunctivitis. J. Vet. Med., Ser. B 43 (1996) 155-161
13. Motha M.X., Frey J., Hansen M.F., Jamaludin R., and Tham K.M. Detection of Mycoplasma conjunctivae in sheep affected with conjunctivitis and infectious keratoconjunctivitis. N. Z. Vet. J. 51 (2003) 186-190