Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis

Cell Research

Volumn 17, Issue 7, 2007, Pages 608-618

  Fu, Guosheng ^a   Ding, Xia ^b,c   Yuan, Kai ^a,b   Aikhionbare, Felix ^b   Yao, Jianhui ^a   Cai, Xin ^a,b   Jiang, Kai ^a,b   Yao, Xuebiao ^a,b  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b MOREHOUSE SCHOOL OF MEDICINE   (United States)

^c BEIJING UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Kinetochore; Microtubule; NEK2A; Phosphorylation; Sgo1

Indexed keywords

CELL CYCLE PROTEIN; GREEN FLUORESCENT PROTEIN; NEK2 PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE; SERINE; SGOL1 PROTEIN, HUMAN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELL LINE; CENTROMERE; CHROMOSOME SEGREGATION; FLUORESCENCE MICROSCOPY; GENETICS; HELA CELL; HUMAN; IMMUNOPRECIPITATION; METABOLISM; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; MOLECULAR GENETICS; PHOSPHORYLATION; PROTEIN BINDING; SEQUENCE HOMOLOGY; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CELL CYCLE PROTEINS; CELL LINE; CHROMOSOME SEGREGATION; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; KINETOCHORES; MICROSCOPY, FLUORESCENCE; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; SEQUENCE HOMOLOGY, AMINO ACID; SERINE;

EID: 34547193780     PISSN: 10010602     EISSN: 17487838     Source Type: Journal    
DOI: 10.1038/cr.2007.55     Document Type: Article

References (21)

1. Cleveland DW, Mao Y, Sullivan KF. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell 2003; 112:407-421.
2. Nicklas RB. The motor for poleward chromosome movement in anaphase is in or near the kinetochore. J Cell Biol 1989; 109:2245-2255.
3. Rieder CL, Alexander SP. Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J Cell Biol 1990; 110:81-95.
4. Yao X, Abrieu A, Zheng Y, et al. CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nat Cell Biol 2000; 2:484-491.
5. Kitajima TS, Kawashima SA, Watanabe Y. The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis. Nature 2004; 427:510-517.
6. Salic A, Waters JC, Mitchison TJ. Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis. Cell 2004; 118:567-578.
7. McGuinness BE, Hirota T, Kudo NR, et al. Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells. PLoS Biol 2005; 3:e86.
8. Katis VL, Galova M, Rabitsch KP, et al. Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332. Curr Biol 2005; 14:560-572.
9. Marston AL, Tham WH, Shah H, et al. A genome-wide screen identifies genes required for centromeric cohesion. Science 2004; 303:1367-1370.
10. Rabitsch KP, Gregan J, Schleiffer A, et al. Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II. Curr Biol 2004; 14:287-301.
11. Nigg EA. Mitotic kinases as regulators of cell division and its checkpoints. Nat Rev Mol Cell Biol 2001; 2:21-32.
12. O'Connell MJ, Krien MJ, Hunter T. Never say never again. The NIMA-related protein kinases in mitotic control. Trends Cell Biol 2003; 13:221-228.
13. Lou Y, Yao J, Zereshki A, et al. NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling. J Biol Chem 2004; 279:20049-20057.
14. Zhou R, Cao X, Watson C, et al. Characterization of protein kinase A-mediated phosphorylation of ezrin in gastric parietal cell activation. J Biol Chem 2003; 278:35651-35659.
15. Yao X, Anderson KL, Cleveland DW. The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules. J Cell Biol 1997; 139:435-447.
16. Xue Y, Zhou F, Lu H, Chen G, Yao X. GPS: a comprehensive www server for phosphorylation sites prediction. Nucl Acids Res 2005; 33:W184-W187.
17. Fu G, Hua S, Ward T, et al. D-box is required for the degradation of human Shugoshin and chromosome alignment. Biochem Biophy Res Commun 2007; 357:672-678.
18. Tang Z, Song Y, Harley SE, et al. Human Bubi protects centromeric sister-chromatid cohesion through Shugoshin during mitosis. Proc Natl Acad Sci USA 2004; 101:18012-18017.
19. Waizenegger IC, Hauf S, Meinke A, Peters JM. Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase. Cell 2005; 103:399-410.
20. Church K, Lin HP. Kinetochore microtubules and chromosome movement during prometaphase in Drosphila melanogaster spermatocytes studied in life and with the electron microscope. Chromosoma 1985; 92:273-282.
21. Ault JG, Nicklas RB. Tension, microtubule rearrangements, and the proper distribution of chromosomes in mitosis. Chromosoma 1989; 98:33-39.