Nanosecond pulsed electric fields induce apoptosis in p53-wildtype and p53-null HCT116 colon carcinoma cells

Apoptosis

Volumn 12, Issue 9, 2007, Pages 1721-1731

  Hall, Emily H ^a,b,c   Schoenbach, Karl H ^b   Beebe, Stephen J ^a  

^a CHILDREN S HOSPITAL OF THE KING S DAUGHTERS   (United States)

^b OLD DOMINION UNIVERSITY   (United States)

^c UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Apoptosis; Bcl 2 family; Caspases; Cytochrome c; Non ionizing radiation; p53

Indexed keywords

CYTOCHROME C; DNA FRAGMENT; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P53;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CARCINOMA CELL; CELL DEATH; CELL FUNCTION; CELL MEMBRANE; CELL SIZE; CELL STRAIN HCT116; CELL STRUCTURE; CELL SURVIVAL; COLON CARCINOMA; CONTROLLED STUDY; ELECTROPORATION; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MITOCHONDRION; NANOSECOND PULSED ELECTRIC FIELD; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PULSED ELECTRIC FIELD; RADIATION; WILD TYPE;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CASPASES; CYTOCHROMES C; DNA FRAGMENTATION; ELECTROMAGNETIC FIELDS; ENZYME INDUCTION; HCT116 CELLS; HUMANS; RADIATION, NONIONIZING; TUMOR SUPPRESSOR PROTEIN P53;

EID: 34547136168     PISSN: 13608185     EISSN: 1573675X     Source Type: Journal    
DOI: 10.1007/s10495-007-0083-7     Document Type: Article

References (32)

1. Beebe SJ, Fox PM, Rec LJ, Buescher ES, Somers K, Schoenbach KH (2002) Nanosecond Pulsed Electric Field (nspef) effects on cells and tissues: apoptosis induction and tumor growth inhibition. IEEE Trans Plasma Sci 30:286-292
2. Beebe SJ, Fox PM, Rec LJ, Willis LK, Schoenbach KH (2003a) Nanosecond, High intensity Pulsed Electric Fields induce apoptosis in human cells. FASEB J. 17:1493-1495
3. Beebe SJ, White J, Blackmore PF, Deng Y, Somers K, Schoenbach KH (2003b) Diverse effects of nanosecond pulsed electric fields on cells and tissues. DNA Cell Biol 22:785-796
4. Vernier PT, Sun Y, Marcu L, Craft CM, Gundersen MA (2004) Nanoelectropulse-induced phosphatidylserine translocation. Biophys J 86:4040-4048
5. Weaver JC (2000) Electroporation of cells and tissues. IEEE Trans Plasma Sci 28(1):24-33
6. Neumann E, Kakorin S et al (1999) Fundamentals of electroporative delivery of drugs and genes. Bioelectrochem Bioenerg 48(1):3-16
7. Weaver JC, Vaughan TE et al (1999) Theory of electrical creation of aqueous pathways across skin transport barriers. Adv Drug Deliv Rev 35(1):21-39
8. Heller R, Jaroszeski MJ et al (1996) Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer 77(5):964-971
9. Mir LM, Morsli N, Garbay JR, Billard V, Robert C, Marty M (2003) Electrochemotherapy: a new treatment of solid tumors. J Exp Clin Cancer Res 22:145-148
10. Cemazar M, Golzio M, Escoffre JM, Couderc B, Sersa G, Teissie J (2006) In vivo imaging of tumor growth after electrochemotherapy with cisplatin. Biochem Biophys Res Commun 348:997-1002
11. Beebe SJ, Blackmore PF, White J, Joshi RP, Schoenbach KH (2004) Nanosecond pulsed electric fields modulate cell function through intracellular signal transduction mechanisms. Physiol Meas 25:1077-1093
12. Nuccitelli R Pliquett U, Chen X, Ford W, Swanson JR, Beebe SJ et al (2006) Nanosecond pulsed electric fields cause melanomas to self-destruct. Biochem Biophys Res Commun 343:351-360
13. Schoenbach KH, Joshi RP, Kolb J, Chen N, Stacey M, Blackmore PF et al (2004) Ultrashort electrical pulses open a new gateway into biological cells. Proc IEEE 92:1122-1137
14. Frey W, White JA, Price RO, Blackmore PF, Joshi RP, Nuccitelli R et al (2006) Plasma membrane voltage changes during Nanosecond Pulsed Electric Field exposure. Biophys J 90:3608-3615
15. Gowrishankar TR, Weaver JC (2006) Electrical behavior and pore accumulation in a multicellular model for conventional and supra- electroporation. Biochem Biophys Res Commun 349:643-653
16. Hu Q, Joshi RP, Schoenbach KH (2005) Simulations of nanopore formation and phosphatidylserine externalization in lipid membranes subjected to a high-intensity, ultrashort electric pulse. Phys Rev E Stat Nonlin Soft Matter Phys 72(3 Pt 1):031902
17. Vernier PT, Ziegler MJ, Sun Y, Chang WV, Gundersen MA, Tieleman DP (2006) Nanopore formation and phosphatidylserine externalization in a phospholipid bilayer at high transmembrane potential. J Am Chem Soc 128:6288-6289
18. Schoenbach KH, Beebe SJ, Buescher ES (2001) Intracellular effect of ultrashort electrical pulses. Bioelectromagnetics 22:440-448
19. Tekle E, Oubrahim H, Dzekunov SM, Kolb JF, Schoenbach KH, Chock PB (2005) Selective field effects on intracellular vacuoles and vesicle membranes with nanosecond electric pulses. Biophys J 89:274-284
20. Vernier PT, Sun Y, Marcu L, Salemi S, Craft CM, Gundersen MA (2003) Calcium bursts induced by nanosecond electric pulses. Biochem Biophys Res Commun 310:286-295
21. Buescher ES, Smith RR, Schoenbach KH (2004) Submicrosecond intense pulsed electric field effects on intracellular free calcium: mechanisms and effects. IEEE Trans Plasma Sci 32:1563-1572
22. White JA, Blackmore PF, Schoenbach KH, Beebe SJ (2004) Stimulation of capacitative calcium entry in HL-60 cells by nanosecond pulsed electric fields. J Biol Chem 279:22964-22972
23. Vernier PT, Sun Y, Marcu L, Craft CM, Gundersen MA (2004) Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts. FEBS Lett 572:103-108
24. Chen N, Schoenbach KH, Kolb JF, Swanson JR, Garner AL, Yang J et al (2004) Leukemic cell intracellular responses to nanosecond electric fields. Biochem Biophys Res Commun 317:421-427
25. Appella E, Anderson CW (2001) Post-translational modifications and activation of p53 by genotoxic stresses. Eur J Biochem 268(10):2764-2772
26. Chipuk JE, Green DR (2003) p53′s believe it or not: lessons on transcription-independent death. J Clin Immunol 23(5):355-361
27. Haupt S, Berger M, Goldberg Z, Haupt Y (2003) Apoptosis - the p53 network. J Cell Sci 116:4077-4085
28. Hall EM, Schoenbach KH, Beebe SJ (2005) Nanosecond pulsed electric fields induce direct electric effects and biological effects on human colon carcinoma cells. DNA Cell Biol 24:283-291
29. Bortner CD, Cidlowski JA (2003) Uncoupling cell shrinkage from apoptosis reveals that Na+ influx is required for volume loss during programmed cell death. J Biol Chem 278:39176-29184
30. Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe SJ et al (2003) Differential effects in cells exposed to Ultra-Short, High Intensity Electric Fields: cell survival, DNA damage, and cell cycle analysis. Mutat Res 5421-2:65-75
31. Vernier PT, Sun Y, Gundersen MA (2006) Nanoelectropulse-driven membrane perturbation and small molecule permeabilization. BMC Cell Biol 19:7:37
32. Joshi RP, Hu Q, Schoenbach KH, Beebe SJ (2004) Energy-landscape-model analysis for irreversibility and its pulse-width dependence in cells subjected to a high-intensity ultrashort electric pulse. Phys Rev E Stat Nonlin Soft Matter Phys 69(5 Pt 1):051901