Aromatase, steroid-5-alpha-reductase type 1 and type 2 mRNA expression in gonads and in brain of Xenopus laevis during ontogeny

General and Comparative Endocrinology

Volumn 153, Issue 1-3, 2007, Pages 280-288

  Urbatzka, R ^a   Lutz, I ^a   Kloas, W ^a,b  

^a LEIBNIZ INSTITUTE OF FRESHWATER ECOLOGY AND INLAND FISHERIES   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

Author keywords

5 Alpha reductase; 5 R; 5 Reductase; Aromatase; Development; Sexual differentiation; Srd5a1; Srd5a2; Xenopus laevis

Indexed keywords

AROMATASE; MESSENGER RNA; STEROID 5 ALPHA REDUCTASE TYPE 1; STEROID 5 ALPHA REDUCTASE TYPE 2; STEROID REDUCTASE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BRAIN; CELL MATURATION; CONTROLLED STUDY; DEVELOPMENTAL STAGE; ENZYME ACTIVITY; GENETIC ANALYSIS; GONAD; HOUSEKEEPING GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; ONTOGENY; OVARY DEVELOPMENT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SEX DIFFERENTIATION; TADPOLE; TESTIS DEVELOPMENT; XENOPUS LAEVIS;

EID: 34547117877     PISSN: 00166480     EISSN: 10956840     Source Type: Journal    
DOI: 10.1016/j.ygcen.2007.01.041     Document Type: Article

References (39)

1. Bassez T., Paris J., Omilli F., Dorel C., and Osborne H.B. Post-transcriptional regulation of ornithine decarboxylase in Xenopus laevis oocytes. Development 110 (1990) 955-962
2. Blazquez M., and Piferrer F. Cloning, sequence analysis, tissue distribution, and sex-specific expression of the neural form of P450 aromatase in juvenile sea bass (Dicentrarchus labrax). Mol. Cell Endocrinol. 219 (2004) 83-94
3. Bögi C., Levy G., Lutz I., and Kloas W. Functional genomics and sexual differentiation in amphibians. Comp. Biochem. Phys. B 133 (2002) 559-570
4. Brennan J., and Capel B. One tissue, two fates: molecular genetic events that underlie testis versus ovary development. Nat. Rev. Genet. 5 (2004) 509-521
5. Chardard D., and Dournon C. Sex reversal by aromatase inhibitor treatment in the newt Pleurodeles waltl. J. Exp. Zool. 283 (1999) 43-50
6. Chen Y., Pan F.C., Brandes N., Afelik S., Solter M., and Pieler T. Retinoic acid signaling is essential for pancreas development and promotes endocrine at the expense of exocrine cell differentiation in Xenopus. Dev. Biol. 271 (2004) 144-160
7. Crews D., Bull J.J., and Wibbels T. Estrogen and sex reversal in turtles: a dose-dependent phenomenon. Gen. Comp. Endocrinol. 81 (1991) 357-364
8. Gimeno S., Komen H., Gerritsen A.G.M., and Bowmer T. Feminisation of young males of the common carp, Cyprinus carpio, exposed to 4-tert-pentylphenol during sexual differentiation. Aquat. Toxicol. 43 (1998) 77-92
9. Hughes I.A. Minireview: sex differentiation. Endocrinology 142 (2001) 3281-3287
10. Kamata R., Takahashi S., and Morita M. Gene expression of sex-determining factors and steroidogenic enzymes in the chicken embryo: influence of xenoestrogens. Gen. Comp. Endocrinol. 138 (2004) 148-156
11. Kato T., Matsui K., Takase M., Kobayashi M., and Nakamura M. Expression of P450 aromatase protein in developing and in sex-reversed gonads of the XX/XY type of the frog Rana rugosa. Gen. Comp. Endocrinol. 137 (2004) 227-236
12. Kitano T., Takamune K., Kobayashi T., Nagahama Y., and Abe S.I. Suppression of P450 aromatase gene expression in sex-reversed males produced by rearing genetically female larvae at a high water temperature during a period of sex differentiation in the Japanese flounder (Paralichthys olivaceus). J. Mol. Endocrinol. 23 (1999) 167-176
13. Kitano T., Takamune K., Nagahama Y., and Abe S.I. Aromatase inhibitor and 17alpha-methyltestosterone cause sex-reversal from genetical females to phenotypic males and suppression of P450 aromatase gene expression in Japanese flounder (Paralichthys olivaceus). Mol. Reprod. Dev. 56 (2000) 1-5
14. Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W., and Richardson P. Genetic and genomic tools for Xenopus research: the NIH Xenopus initiative. Dev. Dyn. 225 (2002) 384-391
15. Kloas W., Lutz I., and Einspanier R. Amphibians as a model to study endocrine disruptors: II. Estrogenic activity of environmental chemicals in vitro and in vivo. Sci. Total Environ. 225 (1999) 59-68
16. Kloas W. Amphibians as a model for the study of endocrine disruptors. Int. Rev. Cytol. 216 (2002) 1-57
17. Kloas W., and Lutz I. Amphibians as model to study endocrine disrupters. J. Chromatogr.A 1130 1 (2006) 16-27
18. Krieg P.A., Varnum S.M., Wormington W.M., and Melton D.A. The mRNA encoding elongation factor-1α (EF-1 alpha) is a major transcript at the midblastula transition in Xenopus. Dev. Biol. 133 (1989) 93-100
19. Kuntz S., Chesnel A., Flament S., and Chardard D. Cerebral and gonadal aromatase expressions are differently affected during sex differentiation of Pleurodeles waltl. J. Mol. Endocrinol. 33 (2004) 717-727
20. Matsuoka M.P., van Nes S., Andersen O., Benfey T.J., and Reith M. Real-time PCR analysis of ovary- and brain-type aromatase gene expression during Atlantic halibut (Hippoglossus hippoglossus) development. Comp. Biochem. Physiol. B 144 (2006) 128-135
21. Miyashita K., Shimizu N., Osanai S., and Miyata S. Sequence analysis and expression of the P450 aromatase and estrogen receptor genes in the Xenopus ovary. J. Steroid Biochem. 75 (2000) 101-107
22. Nef S., and Parada L.F. Hormones in male sexual development. Genes Dev. 14 (2000) 3075-3086
23. Nieuwkoop P.D., and Faber J. Normal Table of Xenopus laevis (Daudin) (1994), Garland Publishing Inc., New York
24. O'Donnell L., Stanton P.G., Wreford N.G., Robertson D.M., and McLachlan R.I. Inhibition of 5-alpha-reductase activity impairs the testosterone-dependent restoration of spermiogenesis in adult rats. Endocrinology 137 (1996) 2703-2710
25. Orn S., Holbech H., Madsen T.H., Norrgren L., and Petersen G.I. Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone. Aquat. Toxicol. 65 (2003) 397-411
26. Osawa N., Oshima Y., and Nakamura M. Molecular cloning of Dmrt1 and its expression in the gonad of Xenopus. Zool. Sci. 22 (2005) 681-687
27. Oshima Y., Hayashi T., Tokunaga S., and Nakamura M. Wnt4 expression in the differentiating gonad of the frog Rana rugosa. Zool. Sci. 22 (2005) 689-693
28. Park J.W., Hecker M., Murphy M.B., Jones P.D., Solomon K.R., Van Der Kraak G., Carr J.A., Smith E.E., Du Preez L., Kendall R.J., and Giesy J.P. Development and optimization of a Q-RT PCR method to quantify CYP19 mRNA expression in testis of male adult Xenopus laevis: comparisons with aromatase enzyme activity. Comp. Biochem. Physiol. B 144 (2006) 18-28
29. Pasmanik M., Schlinger B.A., and Callard G.V. In vivo steroid regulation of aromatase and 5-alpha-reductase in goldfish brain and pituitary. Gen. Comp. Endocrinol. 71 (1988) 175-182
30. Poletti A., Negri-Cesi P., Rabuffetti M., Colciago A., Celotti F., and Martini L. Transient expression of the 5-alpha-reductase type 2 isozyme in the rat brain in late fetal and early postnatal life. Endocrinology 139 (1998) 2171-2178
31. Ross A.J., and Capel B. Signaling at the crossroads of gonad development. Trends Endocrinol. Metab. 16 (2005) 19-25
32. Span P.N., van Bokhoven A., Smals A.G., Sweep C.G., and Schalken J.A. Partial sequencing and tissue distribution of the canine isoforms of steroid 5-alpha-reductase type I and type II. Prostate 44 (2000) 233-239
33. Torres Maldonado L.C., Landa P.A., Moreno M.N., Marmolejo V.A., Meza M.A., and Merchant L.H. Expression profiles of Dax1, Dmrt1, and Sox9 during temperature sex determination in gonads of the sea turtle Lepidochelys olivacea. Gen. Comp. Endocrinol. 129 (2002) 20-26
34. Torres J.M., Gomez-Capilla J.A., Ruiz E., and Ortega E. Semiquantitative RT-PCR method coupled to capillary electrophoresis to study 5-alpha-reductase mRNA isozymes in rat ventral prostate in different androgen status. Mol. Cell Biochem. 250 (2003) 125-130
35. Urbatzka R., Lutz I., Opitz R., and Kloas W. Luteinizing hormone, follicle stimulating hormone, and gonadotropin releasing hormone mRNA expression of Xenopus laevis in response to endocrine disrupting compounds affecting reproductive biology. Gen. Comp. Endocrinol. 146 (2006) 119-125
36. Vallarino M., Mathieu M., DO-Rego J.L., Bruzzone F., Chartrel N., Luu-The V., Pelletier G., and Vaudry H. Ontogeny of 3-{beta}-Hydroxysteroid Dehydrogenase and 5-{alpha}-reductase in the Frog Brain. Ann. N.Y. Acad. Sci. 1040 (2005) 490-493
37. Villalpando I., and Merchant-Larios H. Determination of the sensitive stages for gonadal sex-reversal in Xenopus laevis tadpoles. Int. J. Dev. Biol. 34 (1990) 281-285
38. Yu N.W., Hsu C.Y., Ku H.H., Chang L.T., and Liu H.W. Gonadal differentiation and secretions of estradiol and testosterone of the ovaries of Rana catesbeiana tadpoles treated with 4-hydroxyandrostenedione. J. Exp. Zool. 265 (1993) 252-257
39. Zaccanti F., Petrini S., Rubatta M.L., Stagni A.M., and Giorgi P.P. Accelerated female differentiation of the gonad by inhibition of steroidogenesis in amphibia. Comp. Biochem. Physiol. A 107 (1994) 171-179