Detection of apoptotic cell death in the thymus of dexamethasone treated rats using [123I]Annexin V and in situ oligonucleotide ligation

Journal of Molecular Histology

Volumn 38, Issue 4, 2007, Pages 313-319

  Zavitsanou, Katerina ^a   Nguyen, Vu ^a   Greguric, Ivan ^a   Chapman, Janette ^a   Ballantyne, Patrice ^a   Katsifis, Andrew ^a  

^a AUSTRALIAN NUCLEAR SCIENCE AND TECHNOLOGY ORGANISATION   (Australia)

Author keywords

Annexin V; Apoptosis; Dexamethasone; In situ oligo ligation; Thymus

Indexed keywords

BIOLOGICAL MARKER; DEXAMETHASONE; DNA FRAGMENT; IODINE 123; LIPOCORTIN 5; OLIGONUCLEOTIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; FEMALE; ISOTOPE LABELING; LIGATION; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; THYMUS; TISSUE SECTION;

ANIMALS; ANNEXIN A5; APOPTOSIS; DEXAMETHASONE; GENETIC TECHNIQUES; IODINE RADIOISOTOPES; OLIGONUCLEOTIDES; RATS; THYMUS GLAND; TISSUE DISTRIBUTION;

ANIMALIA; RATTUS;

EID: 34547111256     PISSN: 15672379     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10735-007-9104-7     Document Type: Article

References (24)

1. Ahmed SA, Sriranganathan N (1994) Differential effects of dexamethasone on the thymus and spleen: alterations in programmed cell death, lymphocyte subsets and activation of T cells. Immunopharmacology 28:55-66
2. Allen RT, Hunter WJ, Agrawal DK (1997) Morphological and biochemical characterization and analysis of apoptosis. J Pharmacol Toxicol Methods 37:215-228
3. Belkebir-Mesbah D, Bonnefont-Rousselot D, Frey-Fressart V, Moinard C, Delattre J, Vasson M-P (1999) Consequences of treatment with dexamethasone in rats on the susceptibility of total plasma and isolated lipoprotein fractions to copper oxidation. Endocrine 10:233-242
4. Blankenberg FG, Strauss HW (2001) Will imaging of apoptosis play a role in clinical care? A tale of mice and men. Apoptosis 6:117-123
5. Blankenberg FG, Katsikis PD, Tait JF, Davis E, Naumovski L, Ohtsuki K, Kopiwoda S, Abrams MJ, Darkes M, Robbins RC, Maecker HT, Strauss HW (1998) In vivo detection and imaging of phosphatidylserine expression during programmed cell death. Proc Natl Acad Sci USA 95:6349-6354
6. Claman HN (1972) Corticosteroids and lymphoid cells. N Engl J Med 287:388-397
7. Compton MM, Cidlowski JA (1986) Rapid in vivo effects of glucocorticoids in the integrity of rat lymphocyte genomic deoxyribonucleic acid. Endocrinology 118:38-45
8. Didenko VV, Hornsby PJ (1999) Presence of double-strand breaks with single-base 3′ overhangs in cells undergoing apoptosis but not necrosis. J Cell Biol 26:1251-1258
9. Gavrieli Y, Sherman Y, Ben-Sasson SA (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119:493-501
10. Green AM, Steinmetz ND (2002) Monitoring apoptosis in real time. Cancer J 8:82-92
11. Ichiyoshi H, Kiyozuka Y, Kishimoto Y, Fukuhara S, Tsubura A (2003) Massive telomere loss and telomerase RNA expression in dexamethasone-induced apoptosis in mouse thymocytes. Exp Mol Pathol 75:178-186
12. Keen HG, Dekker BA, Disley L, Hastings D, Lyons S, Reader AJ, Ottewell P, Watson A, Zweit J (2005) Imaging apoptosis in vivo using 124-annexin V and PET. Nucl Med Biol 32:395-402
13. Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239-257
14. 123I-labelled human recombinant annexin V. Biomol Eng 17:51-53
15. Lahorte CMM, Vanderheyden J-L, Steinmetz N, van de Wiele C, Dierckx RA, Slegers G (2004) Apoptosis-detecting radioligands: current state of the art and future perspectives. Eur J Nucl Med Mol Imaging 31:887-919
16. Mann CL, Cidlowski JA (2001) Glucocorticoids regulate plasma membrane potential during rat thymocyte apoptosis in vivo and in vitro. Endocrinology 142:421-429
17. Martin SJ, Reutelinsperger CPM, McGahon AJ, Rader JA, Van Schie RC, Laface DM, Green DR (1995) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Methods 182:1545-1556
18. Ohtsuki K, Akashi K, Aoka Y, Blankenberg FG, Kopiwoda S, Tait JF, Strauss HW (1999) Technetium-99m HYNIC-annexin V: a potential radiopharmaceutical for the in vivo detection of apoptosis. Eur J Nucl Med 26:1251-1258
19. Proskuryakov SY, Konoplyannikov AG, Gabai VL (2003) Necrosis: a specific form of programmed cell death? Exp Cell Res 283:1-16
20. Saraste A, Pulkki K (2000) Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res 45:528-537
21. Schimmer AD, Welsh K, Pinilla C, Wang Z, Krajewska M, Bonneau MJ, Pedersen IM, Kitada S, Scott FL, Bailly-Maitre B, Glinsky G, Scudiero D, Sausville E, Salvesen G, Nefzi A, Ostresh JM, Houghten RA, Reed JC (2004) Small-molecule antagonists of apoptosis suppressor XIAP exhibit broad antitumor activity. Cancer Cell 5:25-35
22. Sun X-M, Dinsdale D, Snowden RT, Cohen GM, Skilleter DN (1992) Characterisation of apoptosis in thymocytes isolated from dexamethasone-treated rats. Biochem Pharmacol 44:2131-2137
23. Surh CD, Sprent J (1994) T-cell apoptosis detected in situ during positive and negative selection in the thymus. Nature 372:100-103
24. van den Eijnde SM, Boshart L, Reutelingsperger CPM, De Zeeuw CI, Vermeij-Keers C (1997) Phosphatidylserine plasma membrane asymmetry in vivo: a pancellular phenomenon which alters during apoptosis. Cell Death Differ 4:311-316