Rapid DNA extraction protocol from stool, suitable for molecular genetic diagnosis of colon cancer

Iranian Biomedical Journal

Volumn 11, Issue 3, 2007, Pages 203-208

  Abbaszadegan, Mohammad Reza ^a   Velayati, Arash ^a   Tavasoli, Alireza ^b   Dadkhah, Ezzat ^a  

^a MASHHAD UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b MASHHAD UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Colon cancer; DNA extraction; Stool

Indexed keywords

CHLOROFORM; DNA; PHENOL; PROTEIN P53;

ARTICLE; CANCER DIAGNOSIS; CLINICAL ARTICLE; CLINICAL PROTOCOL; COLON CANCER; COLONOSCOPY; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DNA EXTRACTION; DNA ISOLATION; EXON; FECES ANALYSIS; GENE AMPLIFICATION; HUMAN; HUMAN GENOME; INTERMETHOD COMPARISON; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PURIFICATION;

ADULT; BASE SEQUENCE; CASE-CONTROL STUDIES; COLONIC NEOPLASMS; COLORECTAL NEOPLASMS; DNA PRIMERS; DNA, NEOPLASM; FECES; GENES, P53; HUMANS; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION;

EID: 34447573001     PISSN: 1028852X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Markowitz, A.J. and Winawer, S.J. (1999) Screening and surveillance for colorectal cancer. Semin. Oncol. 26: 485-498.
2. Vogelstein, B. and Kinzler, K.W. (1993) The multistep nature of cancer. Trends Genet. 9: 138-141.
3. Parsons, R., Myeroff, L.L., Liu, B., Willson, J.K., Markowitz, S.D., Kinzler, K.W. and Vogelstein, B. (1995) Microsatellite instability and mutations of the transforming growth factor beta type II receptor gene in colorectal cancer. Cancer Res. 55: 5548-5550.
4. Sidransky, D., Tokino, T., Hamilton, S.R., Kinzler, K.W., Levin, B., Frost, P. and Vogelstein, B. (1992) Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. Science 256: 102-105.
5. Ahlquist, D.A., Skoletsky, J.E., Boynton, K.A., Harrington, J.J., Mahoney, D.W., Pierceall, W.E., Thibodeau, S.N. and Shuber, A.P. (2000) Colorectal cancer screening by detection of altered human DNA in stool: feasibility of a multitarget assay panel. Gastroenterology 119: 1219-1227.
6. Dong, S.M., Traverso, G., Johnson, C., Geng, L., Favis, R., Boynton, K., Hibi, K., Goodman, SN., D'Allessio, M., Paty, P., Hamilton, SR., Sidransky, D., Barany, F., Levin, B., Shuber, A., Kinzler, K.W., Vogelstein, B. and Jen, J. (2001) Detecting colorectal cancer in stool with the use of multiple genetic targets. J. Natl. Cancer Inst. 93: 858-865.
7. Monteiro, L., Gras, N. and Megraud, F. (2001) Magnetic immuno-PCR assay with inhibitor removal for direct detection of helicobacter pylori in Human Feces. J. Clin. Microbiol. 39 (10): 3778-3780.
8. Deuter, R., Pietsch, S., Hertel, S. and Muller, O. (1995) A method for preparation of fecal DNA suitable for PCR. Nucleic Acids Res. 23: 3800-3801.
9. Machiels, B.M., Ruers, T., Lindhout, M., Hardy, K., Hlavaty, T., Bang, D.D., Somers, V.A., Baeten, C., Von Meyenfeldt, M. and Thunnissen, F.B. (2000) New protocol for DNA extraction of stool. Biotechniques 28: 286-290.
10. Ito, Y., Kobayashi, S., Taniguchi, T., Kainuma, O., Hara, T. and Ochiai, T. (2002) Frequent detection of K-ras mutation in stool samples of colorectal carcinoma patients after improved DNA extraction: comparison with tissue samples. Int. J. Oncol. 20 (6): 1263-1268.
11. Beroud, C. and Soussi, T. (1998) P53 gene mutation: software and database. Nucleic Acids Res. 26: 200-204.
12. Greenfield, L. and White, T.J. (1993) Sample preparation methods. In: Diagnostic Molecular Microbiology: Principles and Applications. (Persing, D.H., Smith, T. F., Tenover, F.C. and White, T.J. eds.), American Society for Microbiology, Washington D.C., pp. 122-137.
13. Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321-4325.
14. Villa, E., Dugani, A., Rebecchi, A.M., Vignoli, A., Grottola, A., Buttafoco, P., Losi, L., Perini, M., Trande, P., Merighi, A., Lerose, R. and Manenti, F. (1996) Identification of subjects at risk for colorectal carcinoma through a test based on K-ras determination in the stool. Gastroenterology 110: 1346-1353.
15. Klaassen, C.H., Jeunink, M.A., Prinsen, C.F., Ruers, T.J., Tan, A.C., Strobbe, L.J. and Thunnissen, F.B. (2003) Quantification of human DNA in feces as a diagnostic test for the presence of colorectal cancer. Clin Chem. 49: 1185-1187.
16. Boynton, K.A., Summerhayes, I.C., Ahlquist, D.A. and Shuber, A.P. (2003) DNA integrity as a potential marker for stool-based detection of colorectal cancer. Clin Chem. 49: 1058-1065.