Hepatitis C virus RNA quantification by the COBAS® AmpliPrep®/COBAS® TaqMan® System: Averages do not tell the whole story

Journal of Clinical Virology

Volumn 39, Issue 4, 2007, Pages 326-327

  Gelderblom, Huub C ^a   Beld, Marcel G H M ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Genotype performance; Hepatitis C virus; Quantification; Real time PCR; TaqMan

Indexed keywords

VIRUS RNA;

GENOTYPE; HEPATITIS C VIRUS; INTERMETHOD COMPARISON; LETTER; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA ANALYSIS; VIRUS DIAGNOSIS;

HEPACIVIRUS; HEPATITIS C; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; TAQ POLYMERASE; VIRAL LOAD;

EID: 34447567345     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2007.05.004     Document Type: Letter

References (5)

1. Beld M., Sentjens R., Rebers S., Weegink C., Weel J., Sol C., et al. Performance of the New Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: conversion to international units and comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, assay. J Clin Microbiol 40 (2002) 788-793
2. Colson P., Motte A., and Tamalet C. Broad differences between the COBAS ampliprep total nucleic acid isolation-COBAS TaqMan 48 hepatitis C virus (HCV) and COBAS HCV monitor v2.0 assays for quantification of serum HCV RNA of non-1 genotypes. J Clin Microbiol 44 (2006) 1602-1603
3. Gelderblom H.C., Menting S., and Beld M.G. Clinical performance of the new Roche COBAS TaqMan HCV Test and High Pure System for extraction, detection and quantitation of HCV RNA in plasma and serum. Antivir Ther 11 (2006) 95-103
4. Mellor J., Hawkins A., and Simmonds P. Genotype dependence of hepatitis C virus load measurement in commercially available quantitative assays. J Clin Microbiol 37 (1999) 2525-2532
5. ® System. J Clin Virol 38 (2007) 326-333