Resolving a DNA sequencing artifact associated with topoisomerase I generated clones in the plasmid pCR®2.1

BioTechniques

Volumn 42, Issue 4, 2007, Pages 458-462

  Forbes, Andrew A ^a   Powell, Thomas H Q ^a   Lobo, Neil F ^a   Feder, Jeffrey L ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE;

ANIMAL; ARTICLE; ARTIFACT; DNA SEQUENCE; METHODOLOGY; MOLECULAR CLONING; PLASMID;

ANIMALS; ARTIFACTS; CLONING, MOLECULAR; DNA TOPOISOMERASES, TYPE I; PLASMIDS; SEQUENCE ANALYSIS, DNA;

EID: 34447539871     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112452     Document Type: Short Survey

References (6)

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3. Shuman, S. 1991. Site-specific interaction of Vaccinia virus topoisomerase I with duplex DNA: minimal DNA substrate for strand cleavage in vitro. J. Biol. Chem. 266:11372-11379.
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6. Hamilton, M.B., E.L. Pincus, A. Di Fiore, and R.C. Fleischer. 1999. Universal linker and ligation procedures for construction genomic DNA libraries enriched for microsatellites. BioTechniques 27:500-507.