N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity

FEBS Journal

Volumn 274, Issue 14, 2007, Pages 3685-3694

  Winterburn, Tim J ^a   Phylip, Lowri H ^a   Bur, Daniel ^b   Wyatt, David M ^a   Berry, Colin ^a   Kay, John ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b ACTELION PHARMACEUTICALS LTD   (Switzerland)

Author keywords

Aspartic proteinase inhibition; IA3; Inhibitor engineering; Pichia aspartic proteinase; Specificity relaxation

Indexed keywords

ASPARTIC PROTEINASE INHIBITOR; EXOPEPTIDASE; FUNGAL ENZYME; HISTIDINE; IA3 POLYPEPTIDE; POLYPEPTIDE; SACCHAROPEPSIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN INTERACTION; SACCHAROMYCES; SACCHAROMYCES CASTELLII; SACCHAROMYCES CEREVISIAE; YEAST;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANTIGENS, PROTOZOAN; ASPARTIC ENDOPEPTIDASES; HYDROGEN-ION CONCENTRATION; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PICHIA; PICHIA PASTORIS; SACCHAROMYCES CASTELLII; SACCHAROMYCES CEREVISIAE;

EID: 34447330438     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.05901.x     Document Type: Article

References (8)

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