The utility of in situ--based methodologies including in situ polymerase chain reaction for the diagnosis and study of viral infections

Human Pathology

Volumn 38, Issue 8, 2007, Pages 1123-1136

  Nuovo, Gerard J ^a  

^a THE OHIO STATE UNIVERSITY WEXNER MEDICAL CENTER   (United States)

Author keywords

Immunohistochemistry; In situ hybridization; In situ PCR; Virus

Indexed keywords

CYTOKINE; VIRUS DNA; VIRUS RNA;

ADENOVIRUS; ARTICLE; BIOPSY; COXSACKIE VIRUS; COXSACKIE VIRUS INFECTION; CYTOMEGALOVIRUS; DNA VIRUS; ENTEROVIRUS; EPSTEIN BARR VIRUS; HISTOPATHOLOGY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA VIRUS; SMALLPOX VIRUS; UTERINE CERVIX CANCER; VARICELLA ZOSTER VIRUS; VIRUS INFECTION; WART VIRUS;

BIOPSY; DNA, VIRAL; HUMANS; IMMUNOHISTOCHEMISTRY; PRIMED IN SITU LABELING; RNA, VIRAL; VIRUS DISEASES; VIRUSES;

EID: 34447303066     PISSN: 00468177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.humpath.2007.04.005     Document Type: Article

References (20)

1. Carvalho de Macedo F., Nicol A.F., Yearsley M., Cordovil Pires A.R., and Nuovo G.J. Histologic, viral, and molecular correlates of dengue fever infection of the liver using highly sensitive immunohistochemistry. Diagn Mol Pathol 15 (2006) 223-228
2. Nuovo G.J. PCR in situ hybridization: protocols and applications. 3rd ed. (1996), Raven Press, New York
3. Nuovo G.J., and Silverstein S.J. Comparison of formalin, buffered formalin, and Bouin's fixation on the detection of human papillomavirus DNA extracted from genital lesions. Lab Invest 59 (1988) 720-724
4. Nuovo G.J., and Forde A. An improved system for reverse transcriptase in situ PCR. J Histotechnol 18 (1995) 295-299
5. Nuovo G.J., Gallery F., MacConnell P., Becker J., and Bloch W. An improved technique for the detection of DNA by in situ hybridization after PCR-amplification. Am J Pathol 139 (1991) 1239-1244
6. Ferenczy A., Mitao M., Nagai N., Silverstein S.J., and Crum C.P. Latent papillomavirus and recurring genital warts. N Engl J Med 313 (1985) 784-788
7. Cosio F., Nuovo M., Porcu P., Caliguiri M., Pelletier R., and Nuovo G.J. Kidney allograft infection with Epstein-Barr virus is highly correlated with post-transplant lymphoproliferative disorder. Am J Transplant 4 (2004) 116-123
8. Nuovo G.J., Becker J., Margiotta M., Burke M., Fuhrer J., and Steigbigel R. In situ detection of PCR-amplified HIV-1 nucleic acids in lymph nodes and peripheral blood in asymptomatic infection and advanced stage AIDS. J Acquir Immune Defic Syndr 7 (1994) 916-923
9. Roychowdhury S., Baiocchi R.A., Peng R., et al. Low dose radiation enhances anti-viral treatment of established Epstein-Barr virus (EBV)-associated primary central nervous system lymphoma. Cancer Res 63 (2003) 965-971
10. Nuovo G.J., DeFaria D., Chanona-Vilchi J., and Zhang Y. Molecular detection of rabies encephalitis and correlation with cytokine expression. Mod Pathol 18 (2005) 62-67
11. Bagasra O., Hauptman S.P., Lischner H.W., Sachs M., and Pomerantz R.J. Detection of human immunodeficiency virus type 1 provirus in mononuclear cells by in situ polymerase chain reaction. N Engl J Med 326 (1992) 1385-1391
12. Embretson J., Zupancic M., Ribas J.L., Burke A., Racz P., Tenner-Racz K., and Haase A.T. Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS. Nature 362 (1993) 359-362
13. Nuovo G.J., Margiotta M., MacConnell P., and Becker J. Rapid in situ detection of PCR-amplified HIV-1 DNA. Diagn Mol Pathol 1 (1992) 98-102
14. Nicol A.F., Fernandes A.T.G., Grinsztejn B., et al. Distribution of immune cell subsets and cytokine producing cells in the uterine cervix of HPV infected women: influence of HIV-1 co-infection. Diag Mol Pathol 14 (2005) 39-47
15. Shevchuk M.M., Nuovo G.J., and Khalife G. HIV in the testis: quantitative histology and HIV localization in germ cells. J Reprod Immunol 41 (1998) 69-79
16. Nuovo G.J., and Alfieri M.L. AIDS dementia is associated with massive, activated HIV-1 infection and concomitant expression of several cytokines. Mol Med 2 (1996) 358-366
17. Cioc A., and Nuovo G.J. Correlation of viral detection with histology in cardiac tissue from patients with sudden, unexpected death. Mod Pathol 15 (2002) 914-922
18. Nuovo G.J., Holly A., Wakely P., and Frankel W. Correlation of histology, viral load, and in situ viral detection in hepatic biopsies from patients with liver transplants secondary to hepatitis C infection. Hum Pathol 33 (2002) 277-284
19. Nuovo G.J., Cooper L.D., and Bartholomew D. Histologic, infectious, and molecular correlates of spontaneous abortion and perinatal mortality. Diagn Mol Pathol 14 (2005) 152-158
20. Satoskar A., Ramirez N., Davis J., Bartholomew D., and Nuovo G.J. Histologic correlates of viral and bacterial infection of the placenta associated with severe morbidity and mortality in the newborn. Hum Pathol 35 (2004) 536-545