Cloning of the glyceraldehyde-3-phosphate dehydrogenase gene from Pseudozyma flocculosa and functionality of its promoter in two Pseudozyma species

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 92, Issue 2, 2007, Pages 245-255

  Neveu, Bertrand ^a   Belzile, Fraņois ^a   Bélanger, Richard R ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Basidiomycete; Glyceraldehyde 3 phosphatedehydrogenase; Greenfluorescent protein; Promoter; Pseudozyma; Recombinantprotein

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN;

3' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; BASIDIOMYCETES; CLASSIFICATION; CONTROLLED STUDY; EPIPHYTE; EUMYCOTA; FUNGUS GROWTH; GENE FUNCTION; GENE SEQUENCE; INTRON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOZYMA ANTARCTICA; PSEUDOZYMA FLOCCULOSA; SEQUENCE HOMOLOGY; TATA BOX; TRANSCRIPTION INITIATION SITE;

AMINO ACID SEQUENCE; BASIDIOMYCOTA; CLONING, MOLECULAR; DNA, FUNGAL; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROMOTER REGIONS (GENETICS); RNA, FUNGAL;

FUNGI; PSEUDOZYMA; PSEUDOZYMA ANTARCTICA; PSEUDOZYMA FLOCCULOSA; USTILAGINALES;

EID: 34447297265     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10482-007-9160-8     Document Type: Article

References (46)

1. Altermann E, Klein JR, Henrich B (1999) Synthesis and automated detection of fluorescently labeled primer extension products. Biotechniques 26:98-101
2. Avis TJ, Cheng YL, Zhao YY, Bolduc S, Neveu B, Anguenot R, Labbé C, Belzile F, Bélanger RR (2005) The potential of Pseudozyma yeastlike epiphytes for the production of heterologous recombinant proteins. Appl Microbiol Biotechnol 69:304-311
3. Bauer R, Oberwinkler F, Vánky K (1997) Ultrastructural markers and systematics in smut fungi and allied taxa. Can J Bot 75:1273-1314
4. Begerow D, Bauer R, Boekhout T (2000) Phylogenetic placements of ustilaginomycetous anamorphs as deduced from nuclear LSU rDNA sequences. Mycol Res 104:53-60
5. Boekhout T (1995) Pseudozyma Bandoni emend. Boekhout, a genus for yeast-like anamorphs of Ustilaginales. J Gen Appl Microbiol 41:359-366
6. Bruns T (2006) A kingdom revised. Nature 443:758-761
7. Chen Y, Roxby R (1997) Identification of a functional CT-element in the Phytophthora infestans piypt1 gene promoter. Gene 198:159-164
8. Cheng YL, Bélanger RR (2000) Protoplast preparation and regeneration from spores of the biocontrol fungus Pseudozyma flocculosa. FEMS Microbiol Lett 190:287-291
9. Cheng YL, Belzile F, Tanguay P, Bernier L, Bélanger RR (2001) Establishment of a gene transfer system for Pseudozyma flocculosa, an antagonistic fungus of powdery mildew fungi. Mol Genet Genomics 266:96-102
10. Dobson MJ, Tuite MF, Roberts NA, Kingsman AJ, Kingsman SM, Perkins RE, Conroy SC, Dunbar B, Fothergill LA (1982) Conservation of high-efficiency promoter sequences in Saccharomyces cerevisiae. Nucleic Acids Res 10:2625-2637
11. Draber P (1991) Quantification of proteins in sample buffer for sodium dodecyl sulfate-polyacrylamide gel-electrophoresis using colloidal silver. Electrophoresis 12:453-456
12. Fortier E (2006) Transformation génétique de Pseudozyma spp. Par électroporation. Master thesis. Université Laval, Québec, QC
13. Godio R, Fouces R, Gudina EJ, Martin JF (2004) Agrobacterium tumefaciens -mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium. Curr Genet 46:287-294
14. Hariharan N, Perry RP (1990) Functional dissection of a mouse ribosomal protein promoter: Significance of the polypyrimidine initiator and an element in the TATA-box region. Proc Natl Acad Sci USA 87:1526-1530
15. Harmsen MC, Schuren FHJ, Moukha SM, Vanzuilen CM, Punt PJ, Wessels JGH (1992) Sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes Schizophyllum commune, Phanerochaete chrysosporium and Agaricus bisporus. Curr Genet 22:447-454
16. Holland JP, Holland MJ (1979) Primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae. J Biol Chem 254:9839-9845
17. Inoh Y, Kitamoto D, Hirashima N, Nakanishi M (2001) Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes. Biochem Biophys Res Commun 289:57-61
18. Iwashita K (2002) Recent studies of protein secretion by filamentous fungi. J Biosci Bioeng 94:530-535
19. Jeong MJ, Park SC, Kwon HB, Byun MO (2000) Isolation and characterization of the gene encoding glyceraldehyde-3-phosphate dehydrogenase. Biochem Biophys Res Commun 278:192-196
20. Kilaru S, Kües U (2005) Comparison of gpd genes and their protein products in basidiomycetes. Fungal Genet Newsl 52:18-23
21. Kolluri R, Torrey TA, Kinniburgh AJ (1992) A CT promoter element binding protein: Definition of a double-strand and a novel single-strand DNA binding motif. Nucleic Acids Res 20:111-116
22. Krebs E, Rafter G, Junge J (1953) Yeast glyceraldehyde-3-phosphate dehydrogenase. II. Yeast protein 2. J Biol Chem 200:479-492
23. Kulakovskaya TV, Shashkov AS, Kulakovskaya EV, Golubev WI (2005) Ustilagic acid secretion by Pseudozyma fusiformata strains. FEMS Yeast Res 5:919-923
24. Kuo CY, Chou SY, Huang CT (2004) Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Appl Microbiol Biotechnol 65:593-599
25. Machida M, Gonzalez TVJ, Boon LK, Gomi K, Jigami YF (1996) Molecular cloning of a genomic DNA for enolase from Aspergillus oryzae. Biosci Biotechnol Biochem 60:161-163
26. McAlister L, Holland MJ (1985) Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260:15013-15018
27. McNeil JB (1988) Functional characterization of a pyrimidine-rich element in the 5′-noncoding region of the yeast iso-1-cytochrome c gene. Mol Cell Biol 8:1045-1054
28. Mimee B, Labbé C, Pelletier R, Bélanger RR (2005) Antifungal activity of flocculosin, a novel glycolipid isolated from Pseudozyma flocculosa. Antimicrob Agents Chemother 49:1597-1599
29. Miyashita A, Crystal RG, Hay JG (1995) Identification of a 27 bp 5′-flanking region element responsible for the low level constitutive expression of the human cytosolic phospholipase A2 gene. Nucleic Acids Res 23:293-301
30. Mooibroek H, Kuipers AGJ, Sietsma JH, Punt PJ, Wessels JGH (1990) Introduction of hygromycin B resistance into Schizophyllum commune: preferential methylation of donor DNA. Mol Gen Genet 222:41-48
31. Neveu B, Labbé C, Bélanger RR (2007) GFP technology for the study of biocontrol agents in tritrophic interactions: A case study with Pseudozyma flocculosa. J Microbiol Methods 68:275-281
32. Neveu B, Michaud M, Belzile F, Bélanger RR (2007) The Pseudozyma flocculosa actin promoter allows the strong expression of a recombinant protein in the Pseudozyma species. Appl Microbiol Biotechnol. DOI 10.1007/s00253-006-0786-7
33. Nitta Y, Miyazaki Y, Nakamura M, Iimura Y, Shishido K, Kajita S, Morohoshi N (2004) Molecular cloning of the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene that contributes to the construction of a new transformation system in Coriolus versicolor. Mycoscience 45:131-136
34. Pesole G, Mignone F, Gissi C, Grillo G, Licciulli F, Liuni S (2001) Structural and functional features of eukaryotic mRNA untranslated regions. Gene 276:73-81
35. Punt PJ, Dingemanse MA, Kuyvenhoven A, Soede RD, Pouwels PH, Van den Hondel CA (1990) Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93:101-109
36. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
37. Santos MA (1991) An improved method for the small scale preparation of bacteriophage DNA based on phage precipitation by zinc chloride. Nucleic Acids Res 19:5442
38. Schillberg S, Tiburzy R, Fischer R (2000) Transient transformation of the rust fungus Puccinia graminis f. sp tritici. Mol Gen Genet 262:911-915
39. Schuren FHJ, Wessels JGH (1994) Highly-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance. Curr Genet 26:179-183
40. Smale ST, Baltimore D (1989) The initiator as a transcription control element. Cell 57:103-113
41. Smith TL (1989) Disparate evolution of yeasts and filamentous fungi indicated by phylogenetic analysis of glyceraldehyde-3-phosphate dehydrogenase genes. Proc Natl Acad Sci USA 86:7063-7066
42. Smith TL, Leong SA (1990) Isolation and characterization of a Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase-encoding gene. Gene 93:111-117
43. Vastag M, Kasza Z, Acs K, Papp T, Schwab H, Vagvolgyi C (2004) Cloning and sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase gene from the zygomycetes fungus Rhizomucor miehei. Antonie Leeuwenhoek 86:111-119
44. Vertesy L, Kurz M, Wink J, Noelken G (2002) Ustilipides, method for the production and the use thereof. US Patent 6,472,158
45. Wolff AM, Arnau J (2002) Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for recombinant protein production. Fungal Genet Biol 35:21-29
46. Yamazaki T, Yasuda T, Miyazaki Y, Okada K, Kajiwara S, Shishido K (2002) A promoter activity in Saccharomyces cerevisiae of the 3′-noncoding region of the basidiomycetous mushroom gene. J Gen Appl Microbiol 48:223-231