Generation of a naïve/synthetic antibody specific to botulinum neurotoxin via motif-grafting

Biotechnology and Bioprocess Engineering

Volumn 12, Issue 3, 2007, Pages 282-288

  Lee, Ji Chul ^a,b   Park, Sun Young ^b   Choi, Cha Yong ^a   Chung, Junho ^b   Lee, Myung Shin ^c,d  

^a Seoul National University   (South Korea)

^b Seoul National University College of Medicine   (South Korea)

^c Armed Forces Medical Research Institute   (South Korea)

^d Eulji University School of Medicine   (South Korea)

Author keywords

Antibody; Botulinum neurotoxin; Peptide library; Phage display

Indexed keywords

BOTULINUM ANTISERUM; BOTULINUM TOXIN A; FORMALDEHYDE; PEPTIDE ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; BIOPANNING; COMBINATORIAL LIBRARY; CONTROLLED STUDY; DNA FLANKING REGION; DRUG SCREENING; DRUG SYNTHESIS; NONHUMAN; PHAGE DISPLAY; PROTEIN MOTIF;

EID: 34447263321     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931105     Document Type: Article

References (22)

1. Gill, D. M. (1982) Bacterial toxins: A table of lethal amounts. Microbiol. Rev. 46: 86-94.
2. Roy, M. J. (2004) Physician's Guide to Terrorist Attack. Humana Press, Totowa, NJ, USA.
3. Pearce, L. B., E. R. First, R. D. MacCallum, and A. Gupta (1997) Pharmacologic characterization of botulinum toxin for basic science and medicine. Toxicon 35: 1373-1412.
4. Singh, B. R., T. Lopes, and M. A. Silvia (1996) Immunochemical characterization of type A botulinum neurotoxin in its purified and complexed forms. Toxicon 34: 267-275.
5. Amon, S. S., R. Schechter, T. V. Inglesby, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eitzen, A. D. Fine, J. Hauer, M. Layton, S. Lillibridge, M. T. Osterholm, T. O'Toole, G. Parker, T. M. Perl, P. K. Russell, D. L. Swerdlow, and K. Tonat (2001) Botulinum toxin as a biological weapon: Medical and public health management. JAMA 285: 1059-1070.
6. Mullaney, B. P., M. G. Pallavicini, and J. D. Marks (2001) Epitope mapping of neutralizing botulinum neurotoxin A antibodies by phage display. Infect. Immun. 69: 6511-6514.
7. Pless, D. D., E. R. Torres, E. K. Reinke, and S. Bavari (2001) High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. Infect. Immun. 69: 570-574.
8. Wu, H. C., C. T. Yeh, Y. L. Huang, L. J. Tarn, and C. C. Lung (2001) Characterization of neutralizing antibodies and identification of neutralizing epitope mimics on the Clostridium botulinum neurotoxin type A. Appl. Environ. Microbiol. 67: 3201-3207.
9. Nowakowski, A., C. Wang, D. B. Powers, P. Amersdorfer, T. J. Smith, V. A. Montgomery, R. Sheridan, R. Blake, L. A. Smith, and J. D. Marks (2002) Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody. Proc. Natl. Acad. Sci. USA 99: 11346-11350.
10. Mah, D. C., W. G. Hu, J. K. Pon, S. A. Masri, R. E. Fulton, P. L. Monette, and L. P. Nagata (2003) Recombinant anti-botulinum neurotoxin A single-chain variable fragment antibody generated using a phage display system. Hybrid. Hybridomics 22: 277-283.
11. Marks, J. D. (2004) Deciphering antibody properties that lead to potent botulinum neurotoxin neutralization. Mov. Disord. 19 Suppl 8: S101-108.
12. Yang, G. H., K. S. Kim, H. W. Kim, S. T. Jeong, G. H. Huh, J. C. Kim, and H. H. Jung (2004) Isolation and characterization of a neutralizing antibody specific to internalization domain of Clostridium botulinum neurotoxin type B. Toxicon 44: 19-25.
13. Chung, J., C. Rader, M. Popkov, Y. M. Hur, H. K. Kim, Y. J. Lee, and C. F. Barbas, 3rd (2004) Integrin alphaiibbeta3-specific synthetic human monoclonal anti bodies and HCDR3 peptides that potently inhibit platelet aggregation. FASEB J. 18: 361-363.
14. Torii, Y., Y. Tokumaru, S. Kawaguchi, N. Izumi, S. Maruyama, M. Mukamoto, S. Kozaki, and M. Takahashi (2002) Production and immunogenic efficacy of botulinum tetravalent (A, B, E, F) toxoid. Vaccine 20: 2556-2561.
15. Barbas, C. F., 3rd, J. D. Bain, D. M. Hoekstra, and R. A. Lerner (1992) Semisynthetic combinatorial antibody libraries: A chemical solution to the diversity problem. Proc. Natl. Acad. Sci. USA 89: 4457-4461.
16. Persson, M. A., R. H. Caothien, and D. R. Burton (1991) Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning. Proc. Natl. Acad. Sci. USA 88: 2432-2436.
17. Barbas, C. F., 3rd, D. R. Burton, J. K, Scott, and G. J. Silverman (2001) Phage Display: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
18. Zdanovsky, A. G., N. V. Karassina, D. Simpson, and M. V. Zdanovskaia (2001) Peptide phage display library as source for inhibitors of clostridial neurotoxins. J. Protein Chem. 20: 73-80.
19. Hynes, R. O. (1987) Integrins: A family of cell surface receptors. Cell 48: 549-554.
20. Ruoslahti, E. and M. D. Pierschbacher (1987) New perspectives in cell adhesion: RGD and integrins. Science 238: 491-497.
21. Albelda, S. M. and C. A. Buck (1990) Integrins and other cell adhesion molecules. FASEB J. 4: 2868-2880.
22. Hynes, R. O. (1992) Integrins: Versatility, modulation, and signaling in cell adhesion. Cell 69: 11-25.