There is no rationale to still rely on outdated, biased tools for quantitative morphology in pulmonary research

European Respiratory Review

Volumn 15, Issue 101, 2006, Pages 105-106

  Fehrenbach, H ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACUTE LUNG INJURY; ANISOTROPY; ASTHMA; BRONCHUS; BRONCHUS BIOPSY; CLINICAL RESEARCH; EDITORIAL; LUNG; LUNG CANCER; LUNG DEVELOPMENT; LUNG EMPHYSEMA; LUNG FIBROSIS; LUNG FUNCTION; LUNG STRUCTURE; LUNG VOLUME; NEPHROLOGIST; NEPHROLOGY; NEUROANATOMY; NEUROSCIENCE; PATHOGENESIS; QUANTITATIVE ANALYSIS; STEREOMETRY; STRUCTURE ACTIVITY RELATION; SYSTEMATIC ERROR; UROLOGY;

EID: 34447134173     PISSN: 09059180     EISSN: None     Source Type: Journal    
DOI: 10.1183/09059180.00010100     Document Type: Editorial

References (27)

1. Weibel ER, Gomez DM. Architecture of the human lung. Use of quantitative methods establishes fundamental relations between size and number of lung structures. Science 1962; 137: 577-585.
2. Weibel ER. Morphometry of the Human Lung. Berlin, Springer-Verlag, 1963.
3. Weibel ER. Stereological Methods - Practical Methods for Biological Morphometry. Volumes 1 and 2. New York, Academic Press, 1979.
4. Weibel ER. Morphometry of the human lung: the state of the art after two decades. Bull Physiopathol Respir (Nancy) 1979; 15: 999-1013.
5. Weibel ER, Hsia CC, Ochs M. How much is there really? Why stereology is essential in lung morphometry. J Appl Physiol 2006; 102: 459-467.
6. Ochs M. A brief update on lung stereology. J Microsc 2006; 222: 188-200.
7. Fahy JV. Remodeling of the airway epithelium in asthma. Am J Respir Crit Care Med 2001; 164: 46-51.
8. Hyde DM, Bolender RP, Harkema JR, Plopper CG. Morphometric approaches for evaluating pulmonary toxicity in mammals: implications for risk assessment. Risk Anal 1994; 14: 293-302.
9. Sterio DC. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc 1984; 134: 127-136.
10. Gundersen HJG, Bendtsen TF, Korbo L, et al. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 1988; 96: 379-394.
11. Gundersen HJG, Bagger P, Bendtsen TF, et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. Review Article. APMIS 1988; 96: 857-881.
12. Nyengaard JR, Gundersen HJG. Sampling for stereology in lungs. Eur Respir Rev 2006; 15: 107-114.
13. West MJ. Stereological methods for estimating the total number of neurons and synapses: issues of precision and bias. Trends Neurosci 1999; 22: 51-61.
14. Benes FM, Lange N. Two-dimensional versus three-dimensional cell counting: a practical perspective. Trends Neurosci 2001; 24: 11-17.
15. Saper CB. Any way you cut it: a new journal policy for the use of unbiased counting methods. J Comp Neurol 1996; 364: 5.
16. Coggeshall RE, Lekan HA. Methods for determining numbers of cells and synapses: a case for more uniform standards of review. J Comp Neurol 1996; 364: 6-15.
17. Madsen KM. The art of counting. J Am Soc Nephrol 1999; 10: 1124-1125.
18. Weibel ER, Parameswaran H, Majumdar A, et al. Morphological quantitation of emphysema: a debate. J Appl Physiol 2006; 100: 1419-1425.
19. Fehrenbach H. Alveolization: does "A" stand for appropriate morphometry? Eur Respir J 2004; 24: 331-332.
20. Fehrenbach H. Design-based counting. Am J Respir Crit Care Med 2004; 169: 1170-1171.
21. Jeffery PK, Holgate S, Wenzel S. Methods for the assessment of endobronchial biopsies in clinical research: application to studies of pathogenesis and the effects of treatment. Am J Respir Crit Care Med 2003; 168: S1-S17.
22. Ochs M, Nyengaard JR, Jung A, et al. The number of alveoli in the human lung. Am J Respir Crit Care Med 2004; 169: 120-124.
23. Ochs M. Stereological analysis of acute lung injury. Eur Respir Rev 2006; 15: 115-121.
24. Hyde DM, Miller LA, Schelegle ES, et al. Asthma: a comparison of animal models using stereological methods. Eur Respir Rev 2006; 15: 122-135.
25. Fehrenbach H. Animal models of pulmonary emphysema: a stereologist's perspective. Eur Respir Rev 2006; 15: 136-147.
26. Hsia CCW. Quantitative morphology of compensatory lung growth. Eur Respir Rev 2006; 15: 148-156.
27. Woodruff PG, Innes AL. Quantitative morphology using bronchial biopsies. Eur Respir Rev 2006; 15: 157-161.