Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers raman spectroscopy

Cytometry Part A

Volumn 71, Issue 7, 2007, Pages 468-474

  Chan, James W ^a,b   Winhold, Heiko ^a   Corzett, Michele H ^a   Ulloa, Joshua M ^a   Cosman, Monique ^a,b   Balhorn, Rod ^a,b   Huser, Thomas ^b,c  

^a LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

E. coli; Glycoprotein; Laser trapping; Optical tweezers; Protein expression; Raman spectroscopy; Single cells

Indexed keywords

ISOPROPYL THIOGALACTOSIDE; MYELIN OLIGODENDROCYTE GLYCOPROTEIN; MYELIN OLIGODENDROCYTE GLYCOPROTEIN[1-120]; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; CELL ACTIVITY; CELL SUSPENSION; CONFOCAL MICROSCOPY; ESCHERICHIA COLI; LASER; LASER TWEEZERS RAMAN SPECTROSCOPY; MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; RAMAN SPECTROMETRY; SIGNAL DETECTION; TIME; VIBRATION;

ANIMALS; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; ISOPROPYL THIOGALACTOSIDE; MODELS, BIOLOGICAL; MYELIN-ASSOCIATED GLYCOPROTEIN; OPTICAL TWEEZERS; RATS; RECOMBINANT PROTEINS; SPECTRUM ANALYSIS, RAMAN;

EID: 34447118151     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20407     Document Type: Article

References (16)

1. Xie CG, Dinno MA, Li YQ. Near-infrared Raman spectroscopy of single optically trapped biological cells. Opt Lett 2002;27:249-251.
2. Chan JW, Esposito AP, Talley CE, Hollars CW, Lane SM, Huser T. Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy. Anal Chem 2004;76:599-603.
3. Cherney DP, Conboy JC, Harris JM. Optical-trapping Raman microscopy detection of single unilamellar lipid vesicles. Anal Chem 2003;75:6621-6628.
4. Singh GP, Volpe G, Creely CM, Grötsch H, Geli IM, Petrov D. The lag phase and G(1) phase of a single yeast cell monitored by Raman microspectroscopy. J Raman Spectrosc 2006; 37:858-864.
5. Xie C, Mace J, Dinno MA, Li YQ, Tang W, Newton RJ, Gemperline PJ. Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy. Anal Chem 2005;77:4390-4397.
6. Xie CG, Chen D, Li YQ. Raman sorting and identification of single living micro-organisms with optical tweezers. Opt Lett 2005;30:1800-1802.
7. Chan JW, Motton D, Rutledge JC, Keim NL, Huser T. Raman spectroscopic analysis of biochemical changes in individual triglyceride-rich lipoproteins in the pre- and postprandial state. Anal Chem 2005;77:5870-5876.
8. Ajito K, Han CX, Torimitsu K. Detection of glutamate in optically trapped single nerve terminals by Raman spectroscopy. Anal Chem 2004;76:2506-2510.
9. Chan JW, Taylor DS, Zwerling T, Lane SM, Ihara K, Huser T. Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophys J 2006;90:648-656.
10. Chen K, Qin YJ, Zheng F, Sun MH, Shi DR. Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells. Opt Lett 2006;31:2015-2017.
11. Xie CG, Li YQ, Tang W, Newton RJ. Study of dynamical process of heat denaturation in optically trapped single microorganisms by near-infrared Raman spectroscopy. J Appl Phys 2003;94:6138-6142.
12. Chen D, Huang SS, Li YQ. Real-time detection of kinetic germination and heterogeneity of single Bacillus spores by laser tweezers Raman spectroscopy. Anal Chem 2006;78:6936-6941.
13. Mannie MD, McConnell TJ, Xie CG, Li YQ. Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy. J Immunol Methods 2005;297: 53-60.
14. Singh GP, Creely CM, Volpe G, Grotsch H, Petrov D. Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy. Anal Chem 2005;77:2564-2568.
15. Koehler NKU, Genain CP, Giesser B, Hauser SL. The human T cell response to myelin oligodendrocyte glycoprotein: A multiple sclerosis family-based study. J Immunol 2002;168:5920-5927.
16. Puppels GJ, Olminkhof JH, Segers-Nolten GM, Otto C, de Mul FF, Greve J. Laser irradiation and Raman-Spectroscopy of single living cells and chromosomes - Sample degradation occurs with 514.5 nm but not with 660 nm laser-light. Exp Cell Res 1991;195: 361-367.