Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

BMC Biotechnology

Volumn 7, Issue , 2007, Pages

  Dmytruk, Kostyantyn V ^a   Smutok, Oleh V ^a   Ryabova, Olena B ^a   Gayda, Galyna Z ^a   Sibirny, Volodymyr A ^b   Schuhmann, Wolfgang ^c   Gonchar, Mykhailo V ^a   Sibirny, Andriy A ^a,b  

^a INSTITUTE OF CELL BIOLOGY   (Ukraine)

^b UNIVERSITY OF RZESZÓW   (Poland)

^c RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMPEROMETRIC SENSORS; ETHANOL; FERMENTATION; PURIFICATION; SATURATION (MATERIALS COMPOSITION); YEAST;

ALCOHOL OXIDASE; HANSENULA POLYMORPHA; MUTATION;

BIOSENSORS;

ALCOHOL; ALCOHOL OXIDASE; ALLYL ALCOHOL; FORMALDEHYDE; METHANOL; ALCOHOL DEHYDROGENASE;

ALLELE; AMPEROMETRIC BIOSENSOR; ARTICLE; BIOSENSOR; BIOTECHNOLOGICAL PROCEDURES; CULTURE MEDIUM; ENZYME ISOLATION; ENZYME SUBSTRATE; GENE EXPRESSION; GENE IDENTIFICATION; GENE ISOLATION; HANSENULA POLYMORPHA; INTERMETHOD COMPARISON; MOLECULAR GENETICS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; WILD TYPE; YEAST; CHEMISTRY; ELECTROCHEMISTRY; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYMOLOGY; EQUIPMENT; EQUIPMENT DESIGN; GENETIC PROCEDURES; GENETICS; INSTRUMENTATION; METABOLISM; METHODOLOGY; MUTATION; PICHIA; PROTEIN ENGINEERING; SENSITIVITY AND SPECIFICITY;

PICHIA ANGUSTA;

ALCOHOL OXIDOREDUCTASES; BASE SEQUENCE; BIOSENSING TECHNIQUES; ELECTROCHEMISTRY; ENZYME ACTIVATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; ETHANOL; FORMALDEHYDE; MOLECULAR SEQUENCE DATA; MUTATION; PICHIA; PROTEIN ENGINEERING; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY;

EID: 34347393855     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-7-33     Document Type: Article

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