High-level expression of cecropin X in Escherichia coli

International Journal of Molecular Sciences

Volumn 8, Issue 6, 2007, Pages 478-491

  Shen, Yi ^a   Xue, Gang Lao ^b   Chen, Yuan ^a   Hong, Zu Zhang ^a   Xian, Xiu Xu ^a  

^a NANJING UNIVERSITY   (China)

^b Zhejiang Medicine Co Ltd   (China)

Author keywords

Cecropin X; Escherichia coli; Fermentation; Inclusion body; Tumorcidal activities

Indexed keywords

CECROPIN; CECROPIN X; COMPLEMENTARY DNA; CYANOGEN BROMIDE; HYBRID PROTEIN; OXYGEN; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBACTERIAL ACTIVITY; ANTINEOPLASTIC ACTIVITY; ARTICLE; CATION TRANSPORT; CELL INCLUSION; CHEMICAL ANALYSIS; ESCHERICHIA COLI; EXPRESSION VECTOR; FERMENTATION OPTIMIZATION; IN VIVO STUDY; LUNG CARCINOMA; MOUSE; NONHUMAN; PEPTIDE ANALYSIS; PROMOTER REGION; TEMPERATURE DEPENDENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 34347390403     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/i8060479     Document Type: Article

References (32)

1. Steiner, H.; Hultmark, D.; Engstrom, A.; Bennich, H.; Boman, H.G. Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 1981, 292, 246-248.
2. Boman, H.G.; Hultmark, D. Cell-free immunity in insects. Ann. Rev. Microbiol. 1987, 41, 103-126.
3. Zhang, S.Q.; Qu, X.M.; Qi, Z.W. Insect immunity and the applied prospect of cecropins. J. Biochem. 1987, 3, 11-18.
4. Zhang, S.Q.; Jia, H.W.; Zhu, Y.D. Ultrastructure observation of K562 leukemia cells treated with antibacterial peptide CM4 component. Prog. Biochem. Biophys. 1997, 24, 159-163.
5. Xu, J.; Zhang, S.Q. The mechanism of antibacterial of antibacterial peptide CMIV component was observed by confocal laser scanning microscope. Prog. Nat. Sci. 2001, 10, 1105-1109.
6. Dou, F.; Xie, W.; Dong, X.Y.; Xu, X.X. The terminal structure plays an important role in the biological activity of cecropin CMIV. Sci. China (Ser. C) 1999, 42, 494-500.
7. Nakajima, Y.K.; Qu, X.M.; Natori, S.J. Interaction between liposomes and Sarcotoxin IA, a potent antibacterial protein of Sarcophaga Peregrina (Flesh Fly). J. Bio. Chem. 1987, 262, 1665-1669.
8. Xie, W.; Qiu, Q.F.; Chen, J.N.; Ma, Z.; Xu, X.X. Chemical synthesis and cloning of cecropin CMIV gene from Chinese silkworm. J. Nanjing Univ. (Nat. Sci.) 1996, 32, 474-478.
9. Xie, W.; Qiu, Q.F.; Dong, X.Y.; Hua, Z.C.; Xu, X.X. Fusion expression of mutated cecropin CM IV in E. coli. Sci. China (Ser. C) 1997, 40, 225-231.
10. Xie, W.; Dou, F.; Qiu, Q.F.; Zhang, J.; Zhang, X.; Xu, X.X. Production and activities against clinical pathogenic bacteria of recombinant CMIV mutant. Prog. Microbiol. Immunol. 1997, 25, 46-49.
11. Wang, L.; Wu, H.H.; Dou, F.; Xie, W.; Xu, X.X. High-level expression of cecropin CMIV in E. coli from a fusion construct containing the human tumor necrosis factor. Biochem. Mol. Biol. Int. 1997, 41, 1051-1056.
12. Seegers, Jos. F.M.L.; Franke, C.M.; Kieweit, R.; Venema, G.; Bron, S. Use of continuous culture for the selection of plasmids with improved segregational stability. Plasmid 1995, 33, 71-77.
13. Nayak, D.P.; Vyas, V.V. Improved stability and expression of a recombinant shuttle plasmid in Escherichia coli during fedbatch cultivation. World. J. Microbiol. Biotechnol. 1999, 15, 65-71.
14. Andersons, D.; Engstrom, A.; Josephson, S.; Hansson, L.; Steiner, H. Biologically active and amidated cecropin produced in a baclovirus expression system from a fusion construct containing the antibody-binding part of protein A. Biochem. J. 1991, 280, 219-224.
15. Hara, S.; Yamakawa, M. Production in Escherichia coli of moricin, a novel type antibacterial peptide from the silkworm, Bombyx mori. Biochem. Biophys. Res. Commun. 1996, 220, 664-669.
16. Harrison, S.J.; McManus, A.M.; Marcus, J.P.; Goulter, K.C.; Green, J.L.; Nielsen, K.J.; Craik, D.J.; Maclean, D.J.; Manners, J.M. Purification and characterization of a plant antimicrobial peptide expressed in Escherichia coli. Protein. Expr. Purif. 1999, 15, 171-177.
17. Lee, J.H.; Kim, J.H.; Hwang, S.W.; Lee, W.J.; Yoon, H.K.; Lee, H.S.; Hong, S.S. High-level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies. Biochem. Biophys. Res. Commun. 2000, 277, 575-580.
18. Hwang, S.W.; Lee, J.H.; Park, H.B.; Pyo, S.H.; So, J.E.; Lee, H.S.; Hong, S.S.; Kim, J.H. A simple method for the purification of an antimicrobial peptide in recombinant Escherichia coli. Mol. Biotechnol. 2001, 18, 193-198.
19. Skosyrev, V.S.; Kulesskiy, E.A.; Yakhnin, A.V.; Temirov, Y.V.; Vinokurov, L.M. Expression of the recombinant antibacterial peptide sarcotoxin IA in Escherichia coli cells. Protein. Expr. Purif. 2003, 28, 350-356.
20. Xu, X.X.; Jin, F.L.; Yu, X.Q.; Ji, S.X.; Wang, J.; Cheng, H.X.; Wang, C.; Zhang, W.Q. Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein. Expr. Purif. 2007, 53, 293-301.
21. Chen, J.J.; Sun, M.; Chen, C.Q.; Lu, F.; Wang, D.B. Construction and application of a high level expression vector containing PR promoter. Prog. Biochem. Biophys. 1996, 26, 495-499.
22. Palomares, A.J.; Vazquez, M.E.; Rodriguez-Llorente, I.D.; Dary, M.; Caviedes, M.A. Plasmid transfer detection in soil using the inducible Lambda PR system fused to eukaryotic luciferase genes. Microbial. Ecology. 2001, 41, 352-359.
23. Lan, P.; Tseng, C.; Lin, M.; Chang, C. Expression and purification of human placenta lactogen in Escherichia coli. Protein. Expr. Purif. 2006, 46, 285-293.
24. Jeong, K.; Choi, J.; Yoo, W.; Keum, K.; Yoo, N.; Lee, S.; Sung, M. Constitutive production of human leptin by fed-batch culture of recombinant rpoS- Escherichia coli. Protein. Expr. Purif. 2004, 36, 150-156.
25. Zhang, X.; Sun, T.; Liu, X.; Gu, D.; Tang, Z. Production of granulocyte-macrophage colony stimulating factor (GM-CSF) by high cell density fermentation of secretory recombination Escherichia coli. Process. Biochem. 1999, 34, 55-58.
26. Peng, L.; Xu, Z.; Fang, X.; Wang, F.; Cen, P. High-level expression of soluble human β-defensin-2 in Escherichia coli. Process. Biochem. 2004, 39, 2199-2205.
27. Datta, A.; Kitson, R.P.; Xue, Y.M.; Al-Atrash, G.; Mazar, A.P.; Jones, T.R.; Goldfarb, R.H. Combined chemoanti-angiogenic cancer therapy against Lewis lung carcinoma (3LL) pulmonary metastases. In vivo. 2002, 16, 451-457.
28. Silvestro, L.; Gupta, K.; Weiser, J.N.; Axelsen, P.H. The concentration-dependent membrane activity of Cecropin A. Biochem 1997, 36, 11452-11460.
29. Kaspar, A.A.; Okada, S.; Kumar, J.; Poulain, F.R.; Drouvalakis, K.A.; Kelekar, A.; Hanson, D.A.; Kluck, R.M.; Hitoshi, Y.; Johnson, D.E.; Froelich, C.J.; Thompson, C.B.; Newmeyer, D.D.; Anel, A.; Clayberger, C.; Krensky, A.M.A distinct pathway of cell-mediated apoptosis initiated by granulysin. J. Immunol. 2001, 167, 350-356.
30. Chen, J.; Xu, X.; Underhill, C.B.; Yang, S.M.; Wang, L.P.; Chen, Y.X.; Hong, S.G.; Creswell, K.; Zhang, L.R. Tachyplesin activates the classic complement pathway to kill tumor cells. Cancer. Res. 2005, 65, 4614-4622.
31. Sambrook, J.; Fritsch, E.F.; Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbour Laboratory Press: New York, 1989; pp. 16-69.
32. Ge, B.S.; Tang, Z.H.; Zhao, F.Q.; Ren, Y.H.; Yang, Y.; Qin, S. Scale-up of fermentation and purification of recombinant allophycocyanin over-expressed in Escherichia coli. Process Biochem. 2005, 40, 3190-3195.