Efficient preparation of an acyclic permutant of kalata B1 from a recombinant fusion protein with thioredoxin

Journal of Biotechnology

Volumn 130, Issue 4, 2007, Pages 378-384

  Cui, Taian ^a   Gao, Yaojun ^b   Liew, Oi Wah ^a   Puah, Chum Mok ^a   Gutte, Bernd ^c  

^a SINGAPORE POLYTECHNIC   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Bacterial expression of thioredoxin ac kalata B1 fusion protein; Disulphide bonds; Enterokinase; Hydrogen peroxide; Recombinant acyclic kalata B1

Indexed keywords

BACTERIAL EXPRESSION; DISULPHIDE BONDS; ENTEROKINASE; RECOMBINANT ACYCLIC KALATA B1; REDUCING POTENTIAL;

AFFINITY CHROMATOGRAPHY; DNA SEQUENCES; ENZYME KINETICS; ESCHERICHIA COLI; MASS SPECTROMETRY;

AMINO ACIDS;

HYBRID PROTEIN; HYDROGEN PEROXIDE; PROTEIN KALATA B1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE MUTATION; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; THERMOSTABILITY;

CHEMICAL FRACTIONATION; CYCLOTIDES; GENETIC ENHANCEMENT; HYDROCARBONS, ACYCLIC; PLANT EXTRACTS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; THIOREDOXINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 34347373046     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.05.006     Document Type: Article

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