Purified and sterilized magnetosomes from Magnetospirillum gryphiswaldense MSR-1 were not toxic to mouse fibroblasts in vitro

Letters in Applied Microbiology

Volumn 45, Issue 1, 2007, Pages 75-81

  Xiang, L ^a   Wei, J ^a   Jianbo, S ^a   Guili, W ^a   Feng, G ^a   Ying, L ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Energy disperse spectroscopy; FT IR; Magnetosome; Magnetospirillum gryphiswaldense; MTT assay

Indexed keywords

CELL CULTURE; DRUG DELIVERY; FIBROBLASTS; GAMMA RAYS; GENE THERAPY; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; PURIFICATION;

BACTERIAL CELLS; ENERGY; ENERGY DISPERSE SPECTROSCOPY; IN-VITRO; LOW POWER; MAGNETOSOMES; MAGNETOSPIRILLUM GRYPHISWALDENSE; MOUSE-FIBROBLASTS; MTT ASSAYS; ULTRA-SONICATION;

FOURIER TRANSFORM INFRARED SPECTROSCOPY;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE;

FOURIER TRANSFORM INFRARED SPECTROSCOPY; MAMMALS; PURIFICATION; TRANSMISSION ELECTRON MICROSCOPY;

BACTERIUM; CELL ORGANELLE; RODENT; TOXICITY; TOXIN;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; CELL DISRUPTION; CELL VIABILITY; CHEMICAL PROCEDURES; CONTROLLED STUDY; CYTOTOXICITY; DRUG DELIVERY SYSTEM; ENERGY EXPENDITURE; FIBROBLAST; FREEZE DRYING; GAMMA RADIATION; GENE DELIVERY SYSTEM; IN VITRO STUDY; INFRARED SPECTROSCOPY; MAGNET; MAGNETOSOME; MAGNETOSPIRILLUM; MAGNETOSPIRILLUM GRYPHISWALDENSE; MOUSE; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; PURIFICATION; QUALITATIVE ANALYSIS; SPECTROSCOPY; STORAGE; STORAGE TEMPERATURE; TOXICOLOGY; TRANSMISSION ELECTRON MICROSCOPY; ULTRASOUND;

BACTERIA (MICROORGANISMS); MAGNETOSPIRILLUM GRYPHISWALDENSE;

EID: 34347357673     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02143.x     Document Type: Article

References (20)

1. Bazylinski, D.A., Garratt-Reed, A.J., Abedi, A. Frankel, R.B. (1993a) Copper association with iron sulfide magnetosomes in a magnetotactic bacterium. Arch Microbiol 160, 35 42.
2. Bazylinski, D.A., Heywood, B.R., Mann, S. Frankel, R.B. (1993b) Fe3O4 and Fe3S4 in a bacterium. Nature 366, 218 219.
3. Berner, R.A. (1967) Thermodynamic stability of sedimentary iron sulfide. Am J Sci 265, 773 785.
4. Blakemore, R.P. (1975) Magnetotactic bacteria. Science 190, 377 379.
5. Gang, F., Wei, J., Ying, L., Jianbo, S., Zhengfang, W., Yangde, Z., Yifeng, P., Weidong, L. et al. (2004) Electron microscopic observation of magnetosome formation in Magnetospirillum gryphiswaldense and its purification. China J Mod Med 14, 45 49.
6. Heyen, U. Schuler, D. (2003) Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor. Appl Microbiol Biotechnol 61, 536 544.
7. Kirschvink, J.L. Gould, J.L. (1981) Biogenic magnetite as a basis for magnetic field detection in animals. Biosystems 13, 181 201.
8. Matsunaga, T. (1991) Application of bacterial magnets. Trends Biotechnol 9, 91 95.
9. Matsunaga, T. Kamiya, S. (1987) Use of magnetic particles isolated from magnetotactic bacterial for enzyme mobilization. Appl Microbiol Biotechnol 26, 328 332.
10. Matsunaga, T., Kawasaki, M., Yu, X., Tsujimura, N. Nakamura, N. (1996) Chemiluminescence enzyme immunoassay using bacterial magnetic particles. Anal Chem 68, 3551 3554.
11. Matsunaga, T., Sato, R., Kamiya, S., Tanaka, T. Takeyama, H. (1999) Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetic complex. J Magn Magn Mater 194, 126 131.
12. Nakamura, N. Matsunaga, T. (1993) Highly sensitive detection of allergen using bacterial magnetic particles: Biosensors. Anal Chim Acta 281, 585 589.
13. Nakamura, N., Hashimoto, K. Matsunaga, T. (1991) Immunoassay method for the determination of immunoglobulin G using bacterial magnetic particles. Anal Chem 63, 268 272.
14. Nakamura, N., Burgess, J.G., Yagiuda, K., Kudo, S., Sakaguchi, T. Matsunaga, T. (1993) Detection and removal of Escherichia coli using fluorescein isothiocyanate conjugated monoclonal antibody immobilized on bacterial magnetic particles. Anal Chem 65, 2036 2039.
15. Schuler, D. Baeuerlein, E. (1996) Iron-limited growth and kinetics of iron uptake in Magnetospirillum gryphiswaldense. Arch Microbiol 166, 301 307.
16. Tanaka, T., Takeda, H., Ueki, F., Obata, K., Tajima, H., Takeyama, H., Goda, Y., Fujimoto, S. et al. (2004) Rapid and sensitive detection of 17 β-estradiol in environmental water using automated immunoassay system with bacterial magnetic particles. J Biotechnol 108, 153 159.
17. Wei, J., Dehua, Z., Ying, L., Jiesheng, T., Zhenfang, W. Jilun, L. (2002) Submerged culture of Magnetospirillum gryphiswaldense under N2-fixing condition and regulation of activity of nitrogenase fixation. Chin Sci Bull 47, 2095 2099.
18. Yang, C., Takeyama, H. Matsunaga, T. (2001a) Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture. J Biosci Bioeng 91, 213 216.
19. Yang, C., Takeyama, H., Tanaka, T. Matsunaga, T. (2001b) Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1. Enzyme Microb Technol 29, 13 19.
20. Yoshino, T. Matsunaga, T. (2006) Efficient and stable display of functional proteins on bacterial magnetic particles using Mms13 as a novel anchor molecule. Appl Microbiol Biotechnol 72, 465 471.