A comparison of experimental methods in molecular chronic rhinosinusitis research

American Journal of Rhinology

Volumn 21, Issue 3, 2007, Pages 373-377

  Ramanathan Jr , Murugappan ^a   Lane, Andrew P ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Epithelial; Immunity; Innate; Methodology; Polymerase chain reaction; Rhinosinusitis; Toll like receptor 9

Indexed keywords

MESSENGER RNA; TOLL LIKE RECEPTOR 9;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; EPITHELIUM CELL; ETHMOID SINUS; FLOW CYTOMETRY; GENE EXPRESSION; HUMAN; HUMAN CELL; INNATE IMMUNITY; NASAL BIOPSY; NOSE MUCOSA; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHINOSINUSITIS;

BIOPSY; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CHRONIC DISEASE; DNA PRIMERS; DNA, COMPLEMENTARY; HUMANS; NASAL MUCOSA; PARANASAL SINUSES; REFERENCE VALUES; RESEARCH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHINITIS; SINUSITIS;

EID: 34347253610     PISSN: 10506586     EISSN: None     Source Type: Journal    
DOI: 10.2500/ajr.2007.21.3034     Document Type: Article

References (16)

1. Kennedy DW. First-line management of sinusitis: A national problem? Overview. Otolaryngol Head Neck Surg. 103:847-854, 1990.
2. Pleis JR, and Coles R. Summary health statistics for U.S. adults: National Health Interview Survey, 1998. Vital Health Stat 10:1-113, 2002.
3. Meltzer EO, Hamilos DL, Hadley JA, et al. Rhinosinusitis: Establishing definitions for clinical research and patient care. Otolaryngol Head Neck Surg. 131(suppl):S1-S62, 2004.
4. Lindbom J, Ljungman AG, Irander K, et al. Phospholipase A2 mRNA expression in the nasal mucosa of healthy subjects and patients with seasonal allergic rhinitis. Rhinology 42:85-91, 2004.
5. Lee JH, Kang HJ, Woo JS, et al. Up-regulation of chemokine ligand 20 in chronic rhinosinusitis. Arch Otolaryngol Head Neck Surg. 132:537-541, 2006.
6. Lee HM, Kang HJ, Woo JS, et al. Upregulation of surfactant protein A in chronic rhinosinusitis. Laryngoscope. 116:328-330, 2006.
7. Vandermeer J, Sha Q, Lane AP, and Schleimer RP. Innate immunity of the sinonasal cavity: Expression of messenger RNA for complement cascade components and toll-like receptors. Arch Otolaryngol Head Neck Surg 130:1374-1380, 2004.
8. Kim J, Myers AC, Chen L, et al. Constitutive and inducible expression of b7 family of ligands by human airway epithelial cells. Am J Respir Cell Mol Biol 33:280-289, 2005 (E-pub Jun 16, 2005).
9. Toskala E, Haataja J, Shirasaki H, and Rautiainen M. Culture of cells harvested with nasal brushing: A method for evaluating ciliary function. Rhinology 43:121-124, 2005.
10. Sachse F, von Eiff C, Stoll W, et al. Induction of CXC chemokines in A549 airway epithelial cells by trypsin and staphylococcal proteases - A possible route for neutrophilic inflammation in chronic rhinosinusitis. Clin Exp Immunol 144:534-542, 2006.
11. Damm M, Quante G, Rosenbohm J, and Rieckmann R. Proinflammatory effects of Staphylococcus aureus exotoxin B on nasal epithelial cells. Otolaryngol Head Neck Surg. 134:245-249, 2006.
12. Lane AP, Saatian B, Yu XY, and Spannhake EW. mRNA for genes associated with antigen presentation are expressed by human middle meatal epithelial cells in culture. Laryngoscope 114:1827-1832, 2004.
13. Dauletbaev N, Gropp R, Frye M, et al. Expression of human beta defensin (HBD-1 and HBD-2) mRNA in nasal epithelia of adult cystic fibrosis patients, healthy individuals, and individuals with acute cold. Respiration 69:46-51, 2002.
14. Claeys S, Van Hoecke H, Holtappels G, et al. Nasal polyps in patients with and without cystic fibrosis: A differentiation by innate markers and inflammatory mediators. Clin Exp Allergy 35:467-472, 2005.
15. Schleimer R, Sha Q, VanderMeer J, et al. Epithelial responses in airway inflammation and immunity. Clin Exp Allergy Rev 4:176-182, 2004.
16. Blander JM, and Medzhitov R. Toll-dependent selection of microbial antigens for presentation by dendritic cells. Nature 440:808-812, 2006.