Uptake and release protocol for assessing membrane binding and permeation by way of isothermal titration calorimetry

Nature Protocols

Volumn 2, Issue 3, 2007, Pages 695-704

  Tsamaloukas, Alekos D ^a   Keller, Sandro ^b   Heerklotz, Heiko ^c   Dan, Leslie ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^c UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL COMPOUND; DRUG; LIGAND; LIPID;

ADDITION REACTION; ARTICLE; DRUG BINDING; DRUG TRANSPORT; EXTRACTION; ISOTHERMAL TITRATION CALORIMETRY; MEMBRANE BINDING; MEMBRANE TRANSPORT; METHODOLOGY; PRIORITY JOURNAL; SOLUTE; CALORIMETRY; CHEMICAL MODEL; CHEMISTRY; EXTRACELLULAR MATRIX; MEMBRANE; PERMEABILITY; TITRIMETRY;

CALORIMETRY; EXTRACELLULAR MATRIX; LIPIDS; MEMBRANES; MODELS, CHEMICAL; PERMEABILITY; TITRIMETRY;

EID: 34347238617     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.98     Document Type: Article

References (35)

1. Seelig, J. & Ganz, P. Nonclassical hydrophobic effect in membrane binding equilibria. Biochemistry 30, 9354-9359 (1991).
2. Seelig, J. Titration calorimetry of lipid-peptide interactions. Biochim. Biophys. Acta 1331, 103-116 (1997).
3. Heerklotz, H. & Seelig, J. Titration calorimetry of surfactant-membrane partitioning and membrane solubilization. Biochim. Biophys. Acta 1508, 69-85 (2000).
4. Lin, L.N., Li, J.Y., Brandts, J.F. & Weiss, R.M. The serine receptor of bacterial chemotaxis exhibits half-site saturation for serine binding. Biochemistry 33, 6564-6570 (1994).
5. 8 by means of isothermal titration calorimetry. J. Phys. Chem. 100, 6764-6774 (1996).
6. Ladbury, J.E. Application of isothermal titration calorimetry in the biological sciences: Things are heating up!. Biotechniques 37 885-887 (2004).
7. Ladbury, J. E. & Doyle, M. L. (eds.) Biocalorimetry Vol. 2 (Wiley-VCH, Weinheim, Germany, 2004).
8. Heerklotz, H. Microcalorimetry of lipid membranes. J. Phys.: Condens. Matter 16, R441-R467 (2004).
9. Heerklotz, H.H., Binder, H. & Epand, R.M. A "release" protocol for isothermal titration calorimetry. Biophys. J. 76, 2606-2613 (1999).
10. Heerklotz, H., Szadkowska, H., Anderson, T. & Seelig, J. The sensitivity of lipid domains to small perturbations demonstrated by the effect of triton. J. Mol. Biol. 329, 793-799 (2003).
11. Tsamaloukas, A., Szadkowska, H. & Heerklotz, H. Nonideal mixing in multicomponent lipid/detergent systems. J. Phys.: Condens. Matter 18 (2006).
12. Hagen, V. et al. Coumarinylmethyl esters for ultrafast release of high concentrations of cyclic nucleotides upon one- and two-photon photolysis. Angew. Chem. Int. Ed. Engl. 44, 7887-7891 (2005).
13. Heerklotz, H. Membrane stress and permeabilization induced by asymmetric incorporation of compounds. Biophys. J. 81, 184-195 (2001).
14. Keller, S., Heerklotz, H. & Blume, A. Monitoring lipid membrane translocation of sodium dodecyl sulfate by isothermal titration calorimetry. J. Am. Chem. Soc. 128, 1279-1286 (2006).
15. Montich, G., Scarlata, S., McLaughlin, S., Lehrmann, R. & Seelig, J. Thermodynamic characterization of the association of small basic peptides with membranes containing acidic lipids. Biochim. Biophys. Acta 1146, 17-24 (1993).
16. Murray, D. et al. Electrostatic properties of membranes containing acidic lipids and adsorbed basic peptides: Theory and experiment. Biophys. J. 77, 3176-3188 (1999).
17. Cambridge, S.B., Geissler, D., Keller, S. & Curten, B. A caged doxycycline analogue for photoactivated gene expression. Angew. Chem. Int. Ed. Engl. 45, 2229-2231 (2006).
18. Gilbert, D. et al. Caged Capsaicins: New tools for the examination of TRPV1 channels in somatosensory neurons. ChemBioChem 8, 89-97 (2007).
19. Bárány-Wallje, E. et al. A critical reassessment of penetratin translocation across lipid membranes. Biophys. J. 89 2513-2521 (2005).
20. Keller, S., Böthe, M., Bienert, M., Dathe, M. & Blume, A. A simple fluorescence-spectroscopic membrane translocation assay. ChemBioChem DOI: 10.1002/cbic.200600553.
21. Tsamaloukas, A., Szadkowska, H., Slotte, P.J. & Heerklotz, H. Interactions of cholesterol with lipid membranes and cyclodextrin characterized by calorimetry. Biophys. J. 89, 1109-1119 (2005).
22. 0. Med. Res. Rev. 19, 333-339 (1999).
23. Spolar, R.S., Ha, J.H. & Record, M.T. Jr. Hydrophobic effect in protein folding and other noncovalent processes involving proteins. Proc. Natl. Acad. Sci. USA 86, 8382-8385 (1989).
24. Kresheck, G.C. & Hargraves, W.A. Thermometric titration studies of the effect of head group, chain length, solvent, and temperature on the thermodynamics of micelle formation. J. Coll. Interf. Sci. 48 481-493 (1974).
25. Hope, M.J., Bally, M.B., Webb, G. & Cullis, P.R. Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potential. Biochim. Biophys. Acta 812, 55-65 (1985).
26. Record, M.T. Jr., Lohman, T.M. & De Haseth, P. Ion effects on ligand nucleic acid interactions. J. Mol. Biol. 107, 145-158 (1976).
27. Mascotti, D.P. & Lohman, T.M. Thermodynamic extent of counterion release upon binding oligolysines to single-stranded nucleic acids. Proc. Natl. Acad. Sci. USA 87, 3142-3146 (1990).
28. Patel, D.R. et al. Calcium-dependent binding of annexin 12 to phospholipid bilayers: Stoichiometry and implications. Biochemistry 40, 7054-7060 (2001).
29. Chellani, M. Isothermal titration calorimetry: Biological applications. Am. Biotechnol. Lab. 17, 14-18 (1999).
30. MacDonald, R.C. et al. Small-volume extrusion apparatus for preparation of large, unilamellar vesicles. Biochim. Biophys. Acta 1061, 297-303 (1991).
31. Wieprecht, T., Apostolov, O., Beyermann, M. & Seelig, J. Thermodynamics of the alpha-helix-coil transition of amphipathic peptides in a membrane environment: Implications for the peptide-membrane binding equilibrium. J. Mol. Biol. 294, 785-794 (1999).
32. Tsamaloukas, A., Szadkowska, H. & Heerklotz, H. Thermodynamic comparison of the interactions of cholesterol with unsaturated phospholipid and sphingomyelins. Biophys. J. 90, 4479-4487 (2006).
33. Keller, M., Kerth, A. & Blume, A. Thermodynamics of interaction of octyl glucoside with phosphatidylcholine vesicles: Partitioning and solubilization as studied by high sensitivity titration calorimetry. Biochim. Biophys. Acta 1326, 178-192 (1997).
34. Heerklotz, H., Binder, H., Lantzsch, G., Klose, G. & Blume, A. Lipid/ detergent interaction thermodynamics as a function of molecular shape. J. Phys. Chem. B 101, 639-645 (1997).
35. Wenk, M.R., Alt, T., Seelig, A. & Seelig, J. Octyl-β-D-glucopyranoside partitioning into lipid bilayers: Thermodynamics of binding and structural changes of the bilayer. Biophys. J. 72, 1719-1731 (1997).