Real-time PCR assay to detect DNA in sera for the diagnosis of deep-seated trichosporonosis

Microbiology and Immunology

Volumn 51, Issue 6, 2007, Pages 633-635

  Mekha, Nanthawan ^a,b   Sugita, Takashi ^b   Ikeda, Reiko ^b   Nishikawa, Akemi ^b   Poonwan, Natteewan ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

Deep seated trichosporonosis; Latex agglutination test; Real time PCR; Trichosporon asahii

Indexed keywords

PLASMID DNA; PLASMID VECTOR;

ARTICLE; BLOOD CULTURE; BLOOD SAMPLING; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DETERMINATION; HUMAN; MYCOSIS; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; TRICHOSPORON ASAHII; TRICHOSPORONOSIS;

TRICHOSPORON ASAHII;

EID: 34250878928     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2007.tb03950.x     Document Type: Article

References (18)

1. Bialek, R., Weiss, M., Bekure-Nemariam, K., Najvar, L.K., Alberdi, M.B., Graybill, J.R., and Reischl, U. 2002. Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assay. Clin. Diagn. Lab. Immunol. 9: 461-469.
2. Halliday, C., Wu, Q.X., James, G., and Sorrell, T. 2005. Development of a nested quantitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens. J. Clin. Microbiol. 43: 5366-5368.
3. Itoh, T., Hosokawa, H., Kohdera, U., Toyazaki, N., and Asad, A. 1996. Disseminated infection with Trichosporon asahii. Mycoses 39: 195-199.
4. Kasai, M., Francesconi, A., Petraitiene, R., Petraitis, V., Kelaher, A.M., Kim, H.S., Meletiadis, J., Sein, T., Bacher, J., and Walsh, T.J. 2006. Use of quantitative real-time PCR to study the kinetics of extracellular DNA released from Candida albicans, with implications for diagnosis of invasive Candidiasis. J. Clin. Microbiol. 44: 143-150.
5. Klingspor, L., and Jalal, S. 2006. Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR. Clin. Microbiol. Infect. 12: 745-753.
6. Loeffler, J., Hebart, H., Brauchle, U., Schumacher, U., and Einsele, H. 2000. Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR. J. Clin. Microbiol. 38: 3830-3833.
7. Marroufi, Y., Heymans, C., De Bruyne, J.M., Duchateau, V., Rodriguez-Villalobos, H., Aoun, M., and Crokaert, F. 2003. Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J. Clin. Microbiol. 41: 3293-3298.
8. McManus, E.J., and Jones, J.M. 1985. Detection of a Trichosporon beigelii antigen cross-reactive with Cryptococcus neoformans capsular polysaccharide in serum from a patient with disseminated Trichosporon infection. J. Clin. Microbiol. 21: 681-685.
9. Moreira-Oliveira, M.S., Mikami, Y., Miyaji, M., Imai, T., Schreiber, A.Z., and Moretti, M.L. 2005. Diagnosis of candidemia by polymerase chain reaction and blood culture: prospective study in a high-risk population and identification of variables associated with development of candidemia. Eur. J. Clin. Microbiol. Infect. Dis. 24: 721-726.
10. Nagai, H., Yamakami, Y., Hashimoto, A., Tokimatsu, I., and Nasu, M. 1999. PCR detection of DNA specific for Trichosporon species in serum of patients with disseminated trichosporonosis. J. Clin. Microbiol. 37: 694-699.
11. Sugita, T., Nakajima, M., Ikeda, R., Matsushima, T., and Shinoda, T. 2002. Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species. J. Clin. Microbiol. 40: 1826-1830.
12. Sugita, T., Nakajima, M., Ikeda, R., Niki, Y., Matsushima, T., and Shinoda, T. 2001. A nested PCR assay to detect DNA in sera for the diagnosis of deep-seated trichosporonosis. Microbiol. Immunol. 45: 143-148.
13. Tashiro, T., Nagai, H., Kamberi, P., Goto, Y., Kikuchi, H., Nasu, M., and Akizuki, S. 1994. Disseminated Trichosporon beigelii infection in patients with malignant diseases: immunohistochemical study and review. Eur. J. Clin. Microbiol. Infect. Dis. 13: 218-224.
14. Tokimatsu, I., Karashima, R., Yamagata, E., Yamakami, Y., Nagai, H., Kadota, J., and Nasu, M. 2003. Pathogenesis of Trichosporon asahii and strategies for infectious control of disseminated trichosporonosis. Jpn. J. Med. Mycol. 44: 181-186.
15. Wahyuningsih, R., Freisleben, H.J., Sonntag, H.G., and Schnitzler, P. 2000. Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J. Clin. Microbiol. 38: 3016-3021.
16. Walsh, T.J., Melcher, G.P., Lee, J.W., and Pizzo, P.A. 1993. Infections due to Trichosporon species: new concepts in mycology, pathogenesis, diagnosis and treatment. Curr. Top. Med. Mycol. 5: 79-113.
17. Willamson, E.C., and Leeming, J.P. 1999. Molecular approaches for the diagnosis and epidemiological investigation of Aspergillus infection. Mycoses 42: 7-10.
18. Yamagata, E., Kamberi, P., Yamakami, Y., Hashimoto, A., and Nasu, M. 2000. Experimental model of progressive disseminated trichosporonosis in mice with latent trichosporonemia. J. Clin. Microbiol. 38: 3260-3266.