Sensitivity and specificity of polymerase chain reaction in Giemsa-stained slides for diagnosis of visceral leishmaniasis in children

Memorias do Instituto Oswaldo Cruz

Volumn 102, Issue 4, 2007, Pages 497-500

  Brustoloni, Yvone Maia ^a   Lima, Rosimar Batista ^a   Da Cunha, Rivaldo Venâncio ^a   Dorval, Maria Elizabeth ^b   Oshiro, Elisa Teruya ^b   De Oliveira, Ana Lúcia Lyrio ^a   Pirmez, Claude ^a  

^a FEDERAL UNIVERSITY OF MATO GROSSO DO SUL   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Diagnosis; Polymerase chain reaction; Visceral leishmaniasis

Indexed keywords

EID: 34250865048     PISSN: 00740276     EISSN: 16788060     Source Type: Journal    
DOI: 10.1590/S0074-02762007005000036     Document Type: Article

References (20)

1. Cascio A, Calattini S, Colomba C, Scalamogna C, Galazzi M, Pizzuto M, Camilli R, Gramiccia M, Titone L, Corbellino M, Antinori S 2002. Polymerase chain reaction in the diagnosis and prognosis of Mediterranean visceral leishmaniasis in immunocompetent children. Pediatrics 10: E27.
2. Cortes S, Rolão N, Ramada J, Campino L 2004. PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers. Trans R Soc Trop Med Hyg 98: 12-17.
3. Costa LMC 1998. Leishmaniose Tegumentar Americana: Uso de Técnicas de Biologia Molecular no Diagnóstico de Infecção de Roedores da Coleção do Museu Nacional, Thesis, Escola Nacional de Saúde Pública, Rio de Janeiro, 70 pp.
4. da Silva ES, Gontijo CM, Pacheco Rda S, Brazil RP 2004. Diagnosis of human visceral leishmaniasis by PCR using blood samples spotted on filter paper. Genet Mol Res 3: 251-257.
5. da Silva MR, Stewart JM, Costa CH 2005. Sensitivity of bone marrow aspirates in the diagnosis of visceral leishmaniasis. Am J Trop Med Hyg 72: 811-814.
6. De Doncker S, Hutse V, Abdellati S, Rijal S, Singh Karki BM, Decuypere S, Jacquet D, Le Ray D, Boelaert M, Koirala S, Dujardin JC 2005. A new PCR-ELISA for diagnosis of visceral leishmaniasis in blood of HIV-negative subjects. Trans R Soc Trop Med Hyg 99: 25-31.
7. Kennedy WP 1984. Novel identification of differences in the kinetoplast DNA of Leishmania isolates by recombinant DNA techniques and in situ hybridisation. Mol Biochem Parasitol 12: 313-325.
8. Lachaud L, Chabbert E, Dubessay P, Dereure J, Lamothe J, Dedet JP, Bastien P 2002b. Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers. Parasitology 125: 197-207.
9. Lachaud L, Chabbert E, Dubessay P, Reynes J, Lamothe J, Bastien P 2001. Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood. J Clin Microbiol 39: 613-617.
10. Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP, Bastien P 2002a. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol 40: 210-215.
11. Lanús EC, Piñero JE, González AC, Valladares B, de Grosso ML, Salomón OD 2005. Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction. Mem Inst Oswaldo Cruz 100: 187-192.
12. Motazedian H, Karamian M, Noyes HA, Ardehali S 2002. DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasitol 96: 31-34.
13. Osman OF, Oskam L, Zijlstra EE, Kroon NC, Schoone GJ, Khalil ET, El-Hassan AM, Kager PA 1997. Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol 35: 2454-2457.
14. Piarroux R, Gambarelli F, Dumon H, Fontes M, Dunan S, Mary C, Toga B, Quilici M 1994. Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral leishmaniasis in immunocompromised patients. J Clin Microbiol 32: 746-749.
15. Pirmez C, da Silva Trajano V, Paes-Oliveira Neto M, da-Cruz AM, Gonçalves-da-Costa SC, Catanho M, Degrave W, Fernandes O 1999. Use of PCR in diagnosis of human American tegumentary leishmaniasis in Rio de Janeiro, Brazil. J Clin Microbiol 37: 1819-23.
16. Rodgers MR, Popper SJ, Wirth DF 1990. Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol 71: 267-75.
17. Salotra P, Sreenivas G, Pogue GP, Lee N, Nakhasi HL, Ramesh V, Negi NS 2001. Development of a species-specific PCR assay for detection of Leishmania donovani in clinical samples from patients with kala-azar and post-kala-azar dermal leishmaniasis. J Clin Microbiol 39: 849-854.
18. Smyth AJ, Ghosh A, Hassan MQ, Basu D, De Bruijn MH, Adhya S, Mallik KK, Barker DC 1992. Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kala-azar patients. Parasitology 105: 183-192.
19. Stark D, Pett S, Marriott D, Harkness J 2006. Post-kala-azar dermal leishmaniasis due to Leishmania infantum in a human immunodeficiency virus type 1-infected patient. J Clin Microbiol 44: 1178-1180.
20. Sundar S 2003. Indian kala-azar-better tools needed for diagnosis and treatment. J Postgrad Med 4: 29-30.