Differential effects of phenylalanine on Rac1, Cdc42, and RhoA expression and activity in cultured cortical neurons

Pediatric Research

Volumn 62, Issue 1, 2007, Pages 8-13

  Zhang, Yongjun ^a   Zhang, Huiwen ^a   Yuan, Xiaobing ^b   Gu, Xuefan ^a,c  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c XINHUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

[No Author keywords available]

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; MESSENGER RNA; PHENYLALANINE; PROTEIN CDC42; RAC1 PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL CULTURE; DENDRITE; DENDRITIC SPINE; EMBRYO; GENE EXPRESSION; NONHUMAN; PHENYLKETONURIA; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SIGNAL TRANSDUCTION;

AMIDES; ANIMALS; CDC42 GTP-BINDING PROTEIN; CELLS, CULTURED; DENDRITES; ENZYME INHIBITORS; HUMANS; NEURONS; PHENYLALANINE; PHENYLKETONURIAS; PYRIDINES; RAC1 GTP-BINDING PROTEIN; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS; RHOA GTP-BINDING PROTEIN; SIGNAL TRANSDUCTION;

EID: 34250847040     PISSN: 00313998     EISSN: None     Source Type: Journal    
DOI: 10.1203/PDR.0b013e31806772be     Document Type: Article

References (39)

1. Moller HE, Weglage J, Bick U, Wiedermann D, Feldmann R, Ullrich K 2003 Brain imaging and proton magnetic resonance spectroscopy in patients with phenylketonuria. Pediatrics 112:1580-1583
2. Huttenlocher PR 2000 The neuropathology of phenylketonuria human and animal studies. Eur J Pediatr 159:s102-s106
3. Kahler SG, Fahey MC 2003 Metabolic disorders and mental retardation. Am J Med Genet C Semin Med Genet 117:31-41
4. Bauman ML, Kemper TL 1982 Morphologic and histoanatomic observations of the brain in untreated human phenylketonuria. Acta Neuropathol (Berl) 58:55-63
5. Horster F, Schwab MA, Sauer SW, Pietz J, Hoffmann GF, Okun JG, Kolker S, Kins S 2006 Phenylalanine reduces synaptic density in mixed cortical cultures from mice. Pediatr Res 59:544-548
6. Schulpis KH, Tjamouranis J, Karikas GA, Michelakakis H, Tsakiris S 2002 In vivo effects of high phenylalanine blood levels on Na+, K+-ATPase, Mg2+-ATPase activities and biogenic amine concentrations in phenylketonuria. Clin Biochem 35:281-285
7. Tsakiris S 2001 Effects of L-phenylalanine on acetylcholinesterase and Na+, K+-ATPase activities in adult and aged rat brain. Mech Ageing Dev 122:491-501
8. Costabeber E, Kessler A, Severo Dutra-Filho C, De Souza Wyse AT, Wajner M, Wannmacher CM 2003 Hyperphenylalaninemia reduces creatine kinase activity in the cerebral cortex of rats. Int J Dev Neurosci 21:111-116
9. Pascucci T, Ventura R, Puglisi-Allegra S, Cabib S 2002 Deficits in brain serotonin synthesis in a genetic mouse model of phenylketonuria. Neuroreport 13:2561-2564
10. Glushakov AV, Dennis DM, Morey TE, Sumners C, Cucchiara RF, Seubert CN, Martynyuk AE 2002 Specific inhibition of N-methyl-D-aspartate receptor function in rat hippocampal neurons by L-phenylalanine at concentrations observed during phenylketonuria. Mol Psychiatry 7:359-367
11. Pietz J, Rupp A, Ebinger F, Rating D, Mayatepek E, Boesch C, Kreis R 2003 Cerebral energy metabolism in phenylketonuria: findings by quantitative in vivo 31P MR spectroscopy. Pediatr Res 53:654-662
12. Yang XW, Gu XF, Chen RG 2000 Toxic effects of phenylacetic acid on cultured rat cortical neurons. Chinese J Neurosci 16:330-332
13. Zhang H, Gu XF 2005 A study of gene expression profiles of cultured embryonic rat neurons induced by phenylalanine. Metab Brain Dis 20:61-72
14. Hausser M, Spruston N, Stuart GJ 2000 Diversity and dynamics of dendritic signaling. Science 290:739-744
15. Matus A 2000 Actin-based plasticity in dendritic spines. Science 290:754-758
16. Kaufmann WE, Moser HW 2000 Dendritic anomalies in disorders associated with mental retardation. Cereb Cortex 10:981-991
17. Ramakers GJ 2002 Rho proteins, mental retardation and the cellular basis of cognition. Trends Neurosci 25:191-199
18. Hall A 1998 Rho GTPases and the actin cytoskeleton. Science 279:509-514
19. Luo L 2000 Rho GTPases in neuronal morphogenesis. Nat Rev Neurosci 1:173-180
20. Pfaffl MW, Horgan GW, Dempfle L 2002 Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36
21. Yuan XB, Jin M, Xu X, Song YQ, Wu CP, Poo MM, Duan SS 2003 Signalling and crosstalk of Rho GTPases in mediating axon guidance. Nat Cell Biol 5:38-45
22. Katoh H, Aoki J, Ichikawa A, Negishi M 1998 p160 RhoA-binding kinase ROKalpha induces neurite retraction. J Biol Chem 273:2489-2492
23. Bito H, Furuyashiki T, Ishihara H, Shibasaki Y, Ohashi K, Mizuno K, Maekawa M, Ishizaki T, Narumiya S 2000 A critical role for a Rho associated kinase, p160ROCK, in determining axon outgrowth in mammalian CNS neurons. Neuron 26:431-441
24. Nakayama AY, Harms MB, Luo L 2000 Small GTPases Rac and Rho in the maintenance of dendritic spines and branches in hippocampal pyramidal neurons. J Neurosci 20:5329-5338
25. Robain O, Wen GY, Wisniewski HM, Shek JW, Loo YH 1981 Purkinje cell dendritic development in experimental phenylketonuria A quantitative analysis. Acta Neuropathol (Berl) 53:107-112
26. Cordero ME, Trejo M, Colombo M, Aranda V 1983 Histological maturation of the neocortex in phenylketonuric rats. Early Hum Dev 8:157-173
27. Lacey DJ 1986 Cortical dendritic spine loss in rat pups whose mothers were prenatally injected with phenylacetate ('maternal PKU' model). Brain Res 392:283-285
28. Williams RS, Hauser SL, Purpura DP, DeLong GR, Swisher CN 1980 Autism and mental retardation: neuropathologic studies performed in four retarded persons with autistic behavior. Arch Neurol 37:749-753
29. Loo YH, Fulton T, Miller K, Wisniewski HM 1980 Phenylacetate and brain dysfunction in experimental phenylketonuria: synaptic development. Life Sci 27:1283-1290
30. Kornguth S, Gilbert-Barness E, Langer E, Hegstrand L 1992 Golgi-Kopsch silver study of the brain of a patient with untreated phenylketonuria, seizures and cortical blindness. Am J Med Genet 44:443-448
31. Lacey DJ 1984 Hippocampal dendritic abnormalities in a rat model of Phenylketonuria. Ann Neurol 16:577-580
32. Threadgill R, Bobb K, Ghosh A 1997 Regulation of dendritic growth and remodeling by rho, rac, and cdc42. Neuron 19:625-634
33. Ramakers GJ 2000 Rho proteins and the cellular mechanisms of mental retardation. Am J Med Genet 94:367-371
34. Scott EK, Reuter JE, Luo L 2003 Small GTPase Cdc42 is required for multiple aspects of dendritic morphogenesis. J Neurosci 23:3118-3123
35. Luo L, Hensch TK, Ackerman L, Barbel S, Jan LY, Jan YN 1996 Differential effects of the Rac GTPase on Purkinje cell axons and dendritic trunks and spines. Nature 379:837-840
36. Ahnert-Hilger G, Holtje M, Grosse G, Pickert G, Mucke C, Nixdorf-Bergweiler B, Boquet P, Hofmann F, Just I 2004 Differential effects of Rho GTPases on axonal and dendritic development in hippocampal neurons. J Neurochem 90:9-18
37. Nobes CD, Hall A 1995 Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 81:53-62
38. Sander EE, Ten Klooster JP, Van Delft S, Van der Kammen RA, Collard JG 1999 Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J Cell Biol 147:1009-1022
39. Yamaguchi Y, Katoh H, Yasui H, Mori K, Negishi M 2001 RhoA inhibits the nerve growth factor-induced Rac1 activation through Rho-associated kinase-dependent pathway. J Biol Chem 276:18977-18983