Evaluation of 5′-deoxy-5′-[F-18]fluorothymidine as a tracer of intracellular thymidine phosphorylase activity

Nuclear Medicine and Biology

Volumn 34, Issue 5, 2007, Pages 471-478

  Grierson, John R ^a   Brockenbrough, J Scott ^a   Rasey, Janet S ^a   Wiens, Linda W ^a   Schwartz, Jeffery L ^a   Jordan, Robert ^a   Vesselle, Hubert ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

5 Deoxy 5 F 18 fluorothymidine; Intracellular tracer; Thymidine phosphorylase

Indexed keywords

2 DEOXY DEXTRO RIBOSE 1ALPHA PHOSPHATE; 5 FLUORO 2,5 DIDEOXY DEXTRO RIBOSE 1ALPHA PHOSPHATE F 18; 5' DEOXY 5' FLUOROTHYMIDINE F 18; CARBOHYDRATE DERIVATIVE; CELL ENZYME; CELL EXTRACT; RADIOPHARMACEUTICAL AGENT; THYMIDINE; THYMIDINE PHOSPHORYLASE; UNCLASSIFIED DRUG; 2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; 3' FLUOROTHYMIDINE; DIAGNOSTIC AGENT;

ARTICLE; BIOACCUMULATION; CARCINOMA CELL; CELL LABELING; CELL METABOLISM; CONCENTRATION RESPONSE; CONTROLLED STUDY; DENSITY FUNCTIONAL THEORY; DRUG ACCUMULATION; DRUG SCREENING; ENZYME ACTIVITY; ENZYME ASSAY; HUMAN; HUMAN CELL; LIPOPHILICITY; LUNG ADENOCARCINOMA; METABOLITE; MOLECULAR IMAGING; MOLECULE; PROTEIN EXPRESSION; RADIOACTIVITY; CELL STRAIN U937; CHEMISTRY; ENZYME ACTIVATION; EVALUATION; METABOLIC CLEARANCE RATE; METABOLISM; NEOPLASM; REPRODUCIBILITY; SCINTISCANNING; SENSITIVITY AND SPECIFICITY; TUMOR CELL LINE;

CELL LINE, TUMOR; DIDEOXYNUCLEOSIDES; ENZYME ACTIVATION; HUMANS; METABOLIC CLEARANCE RATE; NEOPLASMS; RADIOPHARMACEUTICALS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; THYMIDINE PHOSPHORYLASE; U937 CELLS;

EID: 34250766826     PISSN: 09698051     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nucmedbio.2007.03.004     Document Type: Article

References (10)

1. Tozzi M.G., Camici M., Mascia L., Sgarrella F., and Ipata P.L. Pentose phosphates in nucleoside interconversion and catabolism. FEBS J 273 6 (2006) 1089-1101
2. Toi M., Atiqur Rahman M., Bando H., and Chow L.W. Thymidine phosphorylase (platelet-derived endothelial-cell growth factor) in cancer biology and treatment. Lancet Oncol 6 3 (2005) 158-166
3. Langen P., and Kowollik G. 5′-Deoxy-5′-fluorothymidine, a biochemical analogue of thymidine-5′-monophosphate selectively inhibiting DNA synthesis. Eur J Biochem 6 (1968) 344-351
4. Yau E.K., and Coward J.K. Filter chromatography. Aldrichimica Acta 21 4 (1988) 106-107
5. Zhu H.G., Lenzi M., and Schwartz E.L. The Sp1 transcription factor contributes to the tumor necrosis factor-induced expression of the angiogenic factor thymidine phosphorylase in human colon carcinoma cells. Oncogene 21 (2002) 8477-8485
6. Rasey J.S., Grierson J.R., Wiens L.W., Kolb P.D., and Schwartz J.L. Validation of FLT uptake as a measure of thymidine kinase-1 activity in A549 cells. J Nucl Med 43 (2002) 1210-1217
7. 18F]FDT): a bifunctional agent to investigate DNA synthesis rate and a useful tool as a terminal building unit in antisense ODN labelling for PET. J Labelled Compd Radiopharm 37 (1995) 338-340
8. Panova NG, Alexeev C.S, Kuzmicho AS, Shcheveleva EV, Gavryushov SA, Polyakov KM, Substrate specificity of Escherichia coli thymidine phosphorylase. Biochemistry (Moscow), manuscript BM06-089; in press http://www.protein.bio.msu.ru/biokhimiya/inpress/pdf/BM06-089.pdf
9. de Bruin M., Laan A.C., Noordhuis P., Fukushima M., Hoekman K., Pinedo H.M., et al. Rapid disappearance of deoxyribose-1-phosphate in platelet derived endothelial cell growth factor/thymidine phosphorylase overexpressing cells. Biochem Biophys Res Commun 301 (2003) 675-679
10. Bzowska A., Kulikowska E., and Shugar D. Purine nucleoside phosphorylase: properties, functions and clinical aspects. Pharm Ther 88 (2000) 349-425