High-quality RNA extraction from rat pancreas for microarray analysis [3]

Pancreas

Volumn 35, Issue 1, 2007, Pages 98-100

  Kiba, Takayoshi ^a   Kintaka, Yuri ^b   Nakada, Eiko ^b   Suzuki, Yoko ^a   Inoue, Shuji ^b   Ishigaki, Yasuhito ^a  

^a KANAZAWA MEDICAL UNIVERSITY   (Japan)

^b KYORITSU WOMEN'S UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ANIMAL TISSUE; CONTROLLED STUDY; GENE EXPRESSION PROFILING; LETTER; MALE; MICROARRAY ANALYSIS; NONHUMAN; PRIORITY JOURNAL; RAT; RNA DEGRADATION; RNA EXTRACTION; RNA ISOLATION; RNA TRANSCRIPTION; SPECTROPHOTOMETRY;

ANIMALS; MALE; MOLECULAR BIOLOGY; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PANCREAS; RATS; RATS, SPRAGUE-DAWLEY; RIBONUCLEASES; RNA;

EID: 34250722707     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.mpa.0000278690.93930.b6     Document Type: Letter

References (5)

1. Mullin AE, Soukatcheva G, Verchere CB, et al. Application of in situ ductal perfusion to facilitate isolation of high-quality RNA from mouse pancreas. Biotechniques. 2006;40:617-621.
2. Institute of Laboratory Animals Resources. Guide for the Care and Use of Laboratory Animals. Washington, DC: National Academy Press; 1996.
3. Beintema JJ, Campagne RN, Gruber M. Rat pancreatic ribonuclease. I. Isolation and properties. Biochim Biophys Acta. 1973;310:148-160.
4. Chomczynski P, Saachi N. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156-159.
5. Mutter GL, Zahrieh D, Liu C, et al. Comparison of frozen and RNAlater solid tissue storage methods for use in RNA expression microarrays. BMC Genomics. 2004;5:88.