Two-step purification of a highly thermostable alkaline protease from salt-tolerant alkaliphilic Streptomyces clavuligerus strain Mit-1

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 854, Issue 1-2, 2007, Pages 198-203

  Thumar, Jignasha ^a   Singh, S P ^a  

^a SAURASHTRA UNIVERSITY   (India)

Author keywords

Salt tolerant alkaliphilic actinomycete; Streptomyces clavuligerus; Thermostable alkaline protease; Two step purification of alkaline protease

Indexed keywords

BACTERIA; CHROMATOGRAPHY; ION EXCHANGE; MONOMERS; PH EFFECTS; PURIFICATION;

SALT-TOLERANT ALKALIPHILIC ACTINOMYCETE; STREPTOMYCES CLAVULIGERUS; THERMOSTABLE ALKALINE PROTEASE; TWO-STEP PURIFICATION OF ALKALINE PROTEASE;

ENZYMES;

ALKALINE PROTEINASE; BENZYLSULFONYL FLUORIDE; DIETHYLAMINOETHYL CELLULOSE; METAL ION; MONOMER; SERINE; SODIUM CHLORIDE;

ACTINOBACTERIA; ARTICLE; BACTERIAL STRAIN; DENATURATION; ENZYME PURIFICATION; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; SALT TOLERANCE; STREPTOMYCES CLAVULIGERUS; TEMPERATURE; THERMOSTABILITY;

ADAPTATION, PHYSIOLOGICAL; BACTERIAL PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; STREPTOMYCES; TEMPERATURE;

ACTINOBACTERIA (CLASS); STREPTOMYCES CLAVULIGERUS;

EID: 34250718072     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2007.04.023     Document Type: Article

References (30)

1. Han X.Q., and Damodaran S. J. Agric. Food. Chem. 46 (1998) 3596
2. Burg V., and Eijsink V.G.H. Curr. Opin. Biotechnol. 13 (2002) 333
3. Rao M.B., Tanksale A.M., Ghatge M.S., and Deshpande V.V. Microbiol. Mol. Biol. Rev. 62 (1998) 597
4. Mane R.R., and Bapat M. Indian J. Exp. Biol. 39 (2001) 578
5. Huang Q., Peng Y., Li X., Wang H., and Zhang Y. Curr. Microbiol. 46 (2003) 169
6. Joo H.S., and Chang C.S. J. Appl. Microbiol. 98 (2005) 491
7. Setyorini E., Takenaka S., Murakami S., and Aoki K. Biosci. Biotechnol. Biochem. 70 (2006) 433
8. Bakhtiar S., Vevodova J., Hatti-Kaul R., and Su X.D. Acta Crystallgr., Sect. D: Biol. Crystallogr. 59 (2002) 529
9. Gessesse A., Kaul R.H., Gashe B.A., and Mattiasson B. Enz. Microb. Technol. 32 (2003) 519
10. Denizci A.A., Kazan D., Abeln E.C., and Erarslan A. Appl. Microbiol. 96 (2004) 320
11. Okuda M., Sumitomo N., Takimura Y., Ogawa A., Saeki K., Kawai S., Kobayashi T., and Ito S. Extremophiles 8 (2004) 229
12. Sinha R., Joshi R., Dodia M., and Singh S. J. Cell Tissue Res. 7 (2007) 1031
13. Li W.J., Zhang Y.G., Zhang Y.Q., Tang S.K., Xu P., Xu L.H., and Jiang C.L. Int. J. Syst. Evol. Microbiol. 55 (2005) 1329
14. Li W.J., Zhang Y.G., Schumann P., Tian X.P., Zhang Y.Q., Xu L.H., and Jiang C.L. Int. J. Syst. Evol. Microbiol. 56 (2006) 1189
15. Li W.J., Zhang Y.Q., Schumann P., Chen H.H., Hozzein W.N., Tian X.P., Xu L.H., and Jiang C.L. Int. J. Syst. Evol. Microbiol. 56 (2006) 733
16. Lowry O., Rosenbrough W., Farr A., and Rondal R. J. Biol. Chem. 193 (1951) 265
17. Hagihara B. The Enzymes vol. 4 (1958), Academic press, Inc., New York p. 224
18. Laemmli U.K. Nature 227 (1970) 680
19. Lee J.K., Kim Y.O., Kim H.K., Park S.Y., and Oh T.K. Biosci. Biotechnol. Biochem. 60 (1996) 840
20. Tsuchiya K., Ikeda I., Tsuchiya T., and Kimura T. Biosci. Biotechnol. Biochem. 61 (1997) 298
21. Gimenez M.I., Studdert C.A., Sanchez J., and De Castro R.E. Extremophiles 4 (2000) 181
22. Singh J., Batra N., and Sobti R.C. Process Biochem. 36 (2001) 781
23. Studdert C.A., Seitz M.K.H., Gilv M.I.P., Sanchez J.J., and De Castro R.E. J. Basic Microbiol. 41 (2001) 375
24. Miyaji T., Otta Y., Nakagawa T., Watanabe T., Niimura Y., and Tomizuka N. Lett. Appl. Microbiol. 42 (2006) 242
25. Kang S.G., Kim I.S., Rho Y.T., and Lee K.J. Microbiology 141 (1995) 3095
26. M.S. Dodia, Ph.D thesis, Saurashtra University, Rajkot, India, 2005.
27. Patel R.K., Dodia M.S., Joshi R.H., and Singh S.P. Process Biochem. 41 (2006) 2002
28. Banik R.M., and Prakash M. Microbiol. Res. 159 (2004) 135
29. Bockle B., Galunsky B., and Muller R. Appl. Environ. Microbiol. 61 (1995) 3705
30. Gupta A., Roy I., Patel R.K., Singh S.P., Khare S.K., and Gupta M.N. J. Chromatogr. A. 1075 (2005) 103