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34250632018
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note
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Compounds 2 and 12 form gels in water at ∼0.5% w/v and above, while 12 requires NaCl to gel. Compounds 3, 6, and 9 form gels at concentrations as low as 0.2% w/v in the presence of 1 M NaCl.
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34250621411
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note
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Gels became more like voluminous precipitate.
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34250670746
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note
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ANS fluorescence intensity approaches saturation above CMC decreasing the sensitivity to CGC.
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34250641156
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note
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gel.
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34250674499
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For side chain elongation procedure, see:
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0025195578
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34250688373
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note
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Tested for one representative compound. A DABCO salt derived from cholic acid did form a weak gel in 4 M NaCl. But the use of such a high concentration of inorganic salt reduces the general applicability.
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81
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0001484538
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The Physical Chemistry of Cholanic acids
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The CMC of NaDC has been reported to range from 1 to 70 mM, depending on pH, medium, and temperature. (. Nair P.P., and Kritchevsky D. (Eds), Plenum, New York, NY, London ). Our experiments were conducted at 25±1 °C.
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The CMC of NaDC has been reported to range from 1 to 70 mM, depending on pH, medium, and temperature. (. Small D.M. The Physical Chemistry of Cholanic acids. In: Nair P.P., and Kritchevsky D. (Eds). The Bile acids, Chemistry, Physiology and Metabolism Vol. 1 (1971), Plenum, New York, NY, London 249 ). Our experiments were conducted at 25±1 °C.
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34250642374
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note
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ANS fluorescence is quenched in water and the intensity is greatly affected if binding alters the accessibility of water to ANS. ANS has a fluorescence quantum yield of ∼0.002 in water, which increases rapidly to 0.4 when it binds to macromolecules.
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