Measurement of [18F]-fluorodeoxyglucose incorporation into human osteoblast - An experimental method

Cytotechnology

Volumn 54, Issue 1, 2007, Pages 1-4

  Rosenberg, Nahum ^a,b   Bettman, Lise ^a   Rosenberg, Orit ^b   Soudry, Michael ^a,b   Gavish, Moshe ^b   Bar Shalom, Rachel ^a,b  

^a RAMBAM MEDICAL CENTER   (Israel)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

18F fluorodeoxyglucose; Cellular incorporation; Glycolysis; Metabolism; Osteoblast

Indexed keywords

ALKALINE PHOSPHATASE; FLUORODEOXYGLUCOSE F 18; GLUCOSE; PROTOPORPHYRIN;

ARTICLE; CELL CULTURE; CELL FRACTIONATION; CELL METABOLISM; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; OSTEOARTHRITIS; OSTEOBLAST; RADIOACTIVITY; STATISTICAL SIGNIFICANCE;

EID: 34250618731     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-007-9066-3     Document Type: Article

References (14)

1. 2+-induced permeability transition pore opening in rat brain mitochondria. Cell Calcium (in press)
2. Bessey OA, Lowry OH, Brock MJ (1946) A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J Biol Chem 164:321-329
3. Bouillin R (1990) Diabetic bone disease. Calcif Tissue Int 49:155-160
4. Bourke GJ, Daly LE, McGilvray J (1985) Interpretation and Uses of Medical Statistics. Third Edition, Blackwell Scientific Publication: 296-297
5. Brock CS, Meikle SR, Price P (1997) Does fluorine-18 fluorodeoxyglucose metabolic imaging of tumours benefit oncology. Eur J Nucl Med 24:691-705
6. Golshani-Hebroni SG, Bessman SP (1997) Hexokinase binding to mitochondria: a basis for proliferative energy metabolism. J Bioenerg Biomembr 29:331-338
7. Gundle R, Stewart K, Screen J, Beresford JN (1998) Isolation and culture of human bone-derived cells. In: Beresford N, Owen ME (eds) Marrow stromal cell culture. Cambridge university press, Cambridge, UK, pp. 43-66
8. Haberkorn U, Morr I, Oberdorfer F, Bellemann ME, Blatter J, Altmann A, Kahn B, van Kaick G (1994) Fluorodeoxyglucose uptake in vitro: aspects of method and effects of treatment with gemcitabine. J Nucl Med 35:1842-1850
9. Levy JR, Murray E, Manolagas S, Olefsky JM (1986) Demonstration of insulin receptors and modulation of alkaline phosphatase activity by insulin in rat osteoblastic cells. Endocrinology 119:1786-1792
10. Macmahon D (2006) Half-life evaluations for (3)H, (90)Sr, and (90)Y. Appl Radiat Isot 64:1417-1419
11. 18F]-fluorodeoxyglucose. Part I. Biochemical uptake mechanism and its implication for clinical studies. J Cancer Res Clin Oncol 126:549-559
12. Siffert RS (1951) The role of alkaline phosphatase in osteogenesis. J Exp Med 93:415-425
13. Slosman DO, Pittet N, Donath A, Polla BS (1993) Fluorodeoxyglucose cell incorporation as an index of cell proliferation: evaluation of accuracy in cell culture. Eur J Nucl Med 20:1084-1088
14. Thomas DM, Rogers SD, Sleeman MW, Pasquini GM, Brinqhurst FR, Nq KW, Zajac JD, Best JD (1995) Modulation of glucose transport by parathyroid hormone and insulin in UMR 106-01 a clonal osteogenic sarcoma cell line. J Mol Endocrinol 14:263-275