A critical evaluation of a flow cytometer used for detecting enterococci in recreational waters

Journal of Water and Health

Volumn 5, Issue 2, 2007, Pages 295-305

  King, Dawn N ^a   Brenner, Kristen P ^a   Rodgers, Mark R ^b  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Autofluorescence; Enterococci; Flow cytometry; Fluorescence; Membrane filtration; Recreational water

Indexed keywords

ANTIBODY;

BACTERIUM; FILTER; FILTRATION; FLOW CYTOMETRY; FLUORESCENCE; MEMBRANE; MICROBIOLOGY; MICROORGANISM; PATHOGEN; WATER SUPPLY;

ADSORPTION; ANTIBODY LABELING; ARTICLE; AUTOFLUORESCENCE; BACTERIAL COUNT; BACTERIUM DETECTION; CONTROLLED STUDY; ENTEROCOCCUS; ENVIRONMENTAL PROTECTION; FLOW CYTOMETRY; MEMBRANE FILTER; MICROORGANISM DETECTION; RECREATION; SEASHORE; STANDARD; UNITED STATES; WATER SAMPLING;

ENTEROCOCCUS;

EID: 34250616329     PISSN: 14778920     EISSN: None     Source Type: Journal    
DOI: 10.2166/wh.2007.012b     Document Type: Article

References (18)

1. Advanced Analytical Technologies Inc. 2003 Training Manual. U.S. EPA, Ames, IA.
2. American Public Health Association, American Water Works Association and Water Environmental Federation 1998 Standard Methods for the Examination of Water and Wastewater, 20th edn. American Public Health Association, Washington, DC.
3. Felini, D. & Pirovano, B. 1998 The automation of sediment urinalysis using a new urine flow cytometer (UF-100™).Clin. Chem. & Lab. Medicine, 909-917.
4. Ferrari, B. C., Vesey, G., Weir, C., Williams, K. L. & Veal, D. A. 1999 Comparison of Cryptosporidium-specific and Giardia-specific monoclonal antibodies for monitoring water samples. Wat. Res. 33, 1611-1617.
5. Haugland, R. A., Siefring, S. C., Wymer, L. J., Brenner, K. P. & Dufour, A. P. 2005 Comparison of Enterococcus measurements in freshwater at two recreational beaches by quantitative polymerase chain reaction and membrane filter culture analysis. Wat. Res. 39, 559-568.
6. Hoefel, D., Grooby, W. L., Monis, P. T., Andrews, S. & Saint, C. P. 2003 Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques. J. Microbiol. Methods 55, 585-597.
7. Laplace-Builhe, C., Hahne, K., Hunger, W., Tirilly, Y. & Drocourt, J. L. 1993 Application of flow cytometry to rapid microbiological analysis in food and drinks industries. Biol. Cell 78, 123-128.
8. Lindquist, H. D. A., Ware, M., Stetler, R., Wymer, L. & Schaefer, F. W. 2001 A comparison of four fluorescent antibody-based methods for purifying, detecting, and confirming Cryptosporidium parvum in surface waters. J. Parasitol. 87, 1124-1131.
9. Messer, J. W. & Dufour, A. P. 1998 A rapid, specific membrane filtration procedure for enumeration of Enterococci in recreational water. Appl. Environ. Microbiol. 64, 678-680.
10. Patchett, R. A., Back, J. P., Finder, A. C. & Kroll, R. G. 1991 Enumeration of bacteria in pure cultures and in foods using a commercial flow cytometer. Food Microbiology 8, 119-125.
11. + mononuclear cells from the human cervix. J. Immunol. Methods 258, 37-46.
12. Stopa, P. J. & Mastromanolis, S. A. 2001 The use of blue-excitable nucleic-acid dyes for the detection of bacteria in well water using a simple field fluorometer and a flow cytometer. J. Microbiol. Methods 45, 143-153.
13. Tyndall, R. L., Hand, R. E., Jr, Mann, R. C., Evans, C. & Jernigan, R. 1985 Application of flow cytometry to detection and characterization of Legionella spp. Appl. Environ. Microbiol. 49, 852-857.
14. United States Environmental Protection Agency 1986 Ambient water quality criteria for bacteria-1986. Office of Water Regulations and Standards, Criteria and Standards Division, EPA-440/5-84/002, Washington, DC.
15. United States Environmental Protection Agency 1997 Method 1600: Membrane Filter Test Method for Enterococci in Water. Publication EPA-821-R-97-004a, Office of Water, U.S. Environmental Protection Agency, Washington DC.
16. United States Environmental Protection Agency 1999 Plan for Beaches and Recreational Waters. Publication EPA/600/R-98/079, Office of Research and Development, Office of Water, Washington, DC.
17. United States Environmental Protection Agency 2000 Improved enumeration methods for the recreational water quality indicators: Enterococci and Escherichia coli.. Publication 821/R-97/004, Office of Science and Technology, Washington, DC.
18. Yoder, J. S., Blackburn, B. G., Craun, G. F., Hill, V., Levy, D. A., Chen, N., Lee, S. H., Calderon, R. L. & Beach, M. J. 2004 Surveillance for Waterborne-Disease Outbreaks Associated with Recreational Water - United States, 2001-2002. Centers for Disease Control and Prevention, Morbidity and Mortality Weekly Report: Surveillance Summaries - October 22, 2004 53(SS-8), 1-21.