High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A2a receptor

Journal of Structural Biology

Volumn 159, Issue 2 SPEC. ISS., 2007, Pages 166-178

  O'Malley, Michelle A ^a   Lazarova, Tzvetana ^a   Britton, Zachary T ^a   Robinson, Anne S ^a  

^a University of Delaware   (United States)

Author keywords

Adenosine receptor; G protein coupled receptor; Membrane protein expression; Saccharomyces cerevisiae; Yeast

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; ADENOSINE A2A RECEPTOR; CARBOHYDRATE DERIVATIVE; CHOLESTEROL HYDROGEN SUCCINATE; GREEN FLUORESCENT PROTEIN; LIGAND; MEMBRANE PROTEIN; N DODECYL BETA DEXTRO MALTOSIDE; POLYHISTIDINE TAG; RESIN; UNCLASSIFIED DRUG; XANTHINE;

ALPHA HELIX; ARTICLE; BIOPHYSICS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FLUORESCENCE; HETEROLOGOUS EXPRESSION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; ISOLATION PROCEDURE; LIGAND BINDING; LYSIS; MICELLE; MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SOLUBILIZATION; SPECTROSCOPY;

SACCHAROMYCES CEREVISIAE;

EID: 34250350252     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.05.001     Document Type: Article

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