Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

Nature Protocols

Volumn 2, Issue 5, 2007, Pages 1197-1205

  Chen, Qijie ^a   Kon, Junko ^a   Ooe, Hidekazu ^a   Sasaki, Kazunori ^a   Mitaka, Toshihiro ^a  

^a SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN 8; HERMES ANTIGEN; HYALURONIC ACID;

ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; CELL FUNCTION; CELL GROWTH; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; EXTRACELLULAR MATRIX; LIVER CELL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; STEM CELL;

ANIMALS; ANTIGENS, CD44; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; HEPATOCYTES; HYALURONIC ACID; KERATIN-8; RATS; STEM CELLS;

RATTUS;

EID: 34250201821     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.118     Document Type: Article

References (19)

1. Mitaka, T. The current status of primary hepatocyte culture. Int. J. Exp. Pathol. 79, 393-409 (1998).
2. Mitaka, T., Sato, F., Mizuguchi, T., Yokono, T. & Mochizuki, Y. Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells. Hepatology 29, 111-125 (1999).
3. Sidler Pfändler, M.A., Hochli, M., Inderbitzin, D., Meier, P.J. & Stieger, B. Small hepatocytes in culture develop polarized transporter expression and differentiation. J. Cell Sci. 117, 4077-4087 (2004).
4. Sugimoto, S. et al. Morphological changes induced by extracellular matrix are correlated with maturation of rat small hepatocytes. J. Cell. Biochem. 87, 16-28 (2002).
5. Kon, J., Ooe, H., Oshima, H., Kikkawa, Y. & Mitaka, T. Expression of CD44 in rat hepatic progenitor cells. J. Hepatol. 45, 90-98 (2006).
6. Goodison, S., Urquidi, V. & Tarin, D. CD44 cell adhesion molecules. Mol. Pathol. 52, 189-196 (1999).
7. Stern, R. Hyaluronan catabolism: A new metabolic pathway. Eur. J. Cell Biol. 83, 317-325 (2004).
8. Weigel, P. H., Hascall, V.C. & Tammi, M. Hyaluronan synthases. J. Biol. Chem. 272, 13997-14000 (1997).
9. Prehm, P. Hyaluronate is synthesized at plasma membranes. Biochem. J. 220, 597-600 (1984).
10. Tengblad, A. et al. Concentration and relative molecular mass of hyaluronate in lymph and blood. Biochem. J. 236, 521-525 (1986).
11. Mouta Carreira, C. et al. LYVE-1 is not restricted to the lymph vessels: Expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 61, 8079-8084 (2001).
12. Hansen, B. et al. Stabilin-1 and stabilin-2 are both directed into the early endocytic pathway in hepatic sinusoidal endothelium via interactions with clathrin/AP-2, independent of ligand binding. Exp. Cell Res. 303, 160-173 (2005).
13. Saegusa, S., Isaji, S. & Kawarada, Y. Changes in serum hyaluronic acid levels and expression of CD44 and CD44 mRNA in hepatic sinusoidal endothelial cells after major hepatectomy in cirrhotic rats. World J. Surg. 26, 694-699 (2002).
14. Mitaka, T., Sattler, G.L. & Pitot, H.C. Amino acid-rich medium (Leibovitz L-15) enhances and prolongs proliferation of primary cultured rat hepatocytes in the absence of serum. J. Cell. Physiol. 147 495-504 (1991).
15. Kreamer, B.L. et al. Use of a low-speed, isodensity percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cell. Dev. Biol. 22, 201-211 (1986).
16. Miyamoto, S., Hirata, K., Sugimoto, S., Harada, K. & Mitaka, T. Expression of cytochrome P450 enzymes in hepatic organoid reconstructed by rat small hepatocytes. J. Gastroenterol. Hepatol. 20, 865-872 (2005).
17. Ikeda, S. et al. Proliferation of rat small hepatocytes after long-term cryopreservation. J. Hepatol. 37, 7-14 (2002).
18. Ooe, H. et al. Cytochrome P450 expression of cultured rat small hepatocytes after long-term cryopreservation. Drug Metab. Dispos. 34, 1667-1671 (2006).
19. Mitaka, T., Norioka, K., Sattler, G.L., Pitot, H.C. & Mochizuki, Y. Effect of age on the formation of small-cell colonies in cultures of primary rat hepatocytes. Cancer Res. 53, 3145-3148 (1993).