Regeneration of the epithelium in organ-cultured donor corneas with extended post-mortem time

Acta Ophthalmologica Scandinavica

Volumn 85, Issue 4, 2007, Pages 371-376

  Slettedal, Jon Klokk ^a   Lyberg, Torstein ^a   Ramstad, Hanne ^a   Beraki, Kahsai ^a,b   Nicolaissen, Bjørn ^a,b  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

Donor cornea; Epithelium; Organ culture; Post mortem time; Scanning electron microscopy; Transplantation

Indexed keywords

ANTIBODY; CYTOKERATIN; CYTOKERATIN 19; CYTOKERATIN 3; PROTEIN P63; UNCLASSIFIED DRUG; VIMENTIN;

ARTICLE; CELL CULTURE MONITORING; CELL VIABILITY; CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEA PRESERVATION; CULTURE MEDIUM; EPITHELIUM CELL; EYEBANK; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; ORGAN CULTURE; ORGAN DONOR; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TIME; TISSUE REGENERATION;

ADULT; AGED; AGED, 80 AND OVER; BIOLOGICAL MARKERS; CELL SURVIVAL; CORNEA; EPITHELIUM, CORNEAL; FEMALE; HUMANS; MALE; MICROSCOPY, ELECTRON, SCANNING; MIDDLE AGED; ORGAN CULTURE TECHNIQUES; ORGAN PRESERVATION; REGENERATION; TIME FACTORS; TISSUE DONORS;

EID: 34250175430     PISSN: 13953907     EISSN: 16000420     Source Type: Journal    
DOI: 10.1111/j.1600-0420.2006.00840.x     Document Type: Article

References (31)

1. Andersen J & Ehlers N (1988): The influence of donor age and post mortem time on corneal graft survival and thickness when employing banked donor material (a five-year follow-up). Acta Ophthalmol (Copenh) 66: 313-317.
2. Crewe JM & Armitage WJ (2001): Integrity of epithelium and endothelium in organ-cultured human corneas. Invest Ophthalmol Vis Sci 42: 1757-1761.
3. Di Iorio E, Barbaro V, Ruzza A, Ponzin D, Pellegrini G & De Luca M (2005): Isoforms of DeltaNp63 and the migration of ocular limbal cells in human corneal regeneration. Proc Natl Acad Sci USA 102: 9523-9528.
4. Dua HS, Joseph A, Shanmuganathan VA & Jones RE (2003): Stem cell differentiation and the effects of deficiency. Eye 17: 877-885.
5. Dua HS, Watson NJ, Mathur RM & Forrester JV (1993): Corneal epithelial cell migration in humans: 'hurricane and blizzard keratopathy'. Eye 7: 53-58.
6. Ebato B, Friend J & Thoft RA (1987): Comparison of central and peripheral human corneal epithelium in tissue culture. Invest Ophthalmol Vis Sci 28: 1450-1456.
7. Egarth M, Hellkvist J, Claesson M, Hanson C & Stenevi U (2005): Long-term survival of transplanted human corneal epithelial cells and corneal stem cells. Acta Ophthalmol Scand 83: 456-461.
8. Ehlers (2002): Corneal bTnking and grafting: the background to the Danish Eye Bank System, where corneas await their patients. Acta Ophthalmol Scand 80: 572-578.
9. Haaskjold E, Sandvig KU, Bjerknes R & Kravik K (1992): The early cell kinetic response during healing of corneal epithelial wounds. Ophthalmic Surg 23: 680-684.
10. James SE, Rowe A, Ilari L, Daya S & Martin R (2001): The potential for eye bank limbal rings to generate cultured corneal epithelial allografts. Cornea 20: 488-494.
11. Kasper M (1992): Patterns of cytokeratins and vimentin in guinea pig and mouse eye tissue: Evidence for regional variations in intermediate filament expression in limbal epithelium. Acta Histochem 93: 319-332.
12. Kasper M, Moll R, Stosiek P & Karsten U (1988): Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry 89: 369-377.
13. Kivela T & Uusitalo M (1998): Structure, development and function of cytoskeletal elements in non-neuronal cells of the human eye. Prog Retin Eye Res 17: 385-428.
14. Lindstrom RL, Doughman DJ, Van Horn DL, Dancil D & Harris JE (1976): A metabolic and electron microscopic study of human organ-cultured cornea. Am J Ophthalmol 82: 72-82.
15. Lu L, Reinach PS & Kao WW (2001): Corneal epithelial wound healing. Exp Biol Med (Maywood) 226: 653-664.
16. Means TL, Geroski DH, L'Hernault N, Grossniklaus HE, Kim T & Edelhauser HF (1996): The corneal epithelium after optisol-GS storage. Cornea 15: 599-605.
17. Meyer RF & Bobb KC (1980): Corneal epithelium in penetrating keratoplasty. Am J Ophthalmol 90: 142-147.
18. Pellegrini G, Dellambra E, Golisano O et al. (2001): P63 identifies keratinocyte stem cells. Proc Natl Acad Sci USA 98: 3156-3161.
19. Pfister RR (1975): The healing of corneal epithelial abrasions in the rabbit: A scanning electron microscope study. Invest Ophthalmol 14: 648-661.
20. Reim M, Althoff C & von Mulert B (1988): Effect of low temperatures on the metabolism of corneal cultures. Graefes Arch Clin Exp Ophthalmol 226: 353-356.
21. Sandvig KU, Haaskjold E, Bjerknes R, Refsum SB & Kravik K (1994): Cell kinetics of conjunctival and corneal epithelium during regeneration of different-sized corneal epithelial defects. Acta Ophthalmol (Copenh) 72: 43-48.
22. Sandvig KU, Nicolaissen B Jr & Haaskjold E (1991): Morphology and proliferation of human corneal epithelium in organ culture. Acta Ophthalmol (Copenh) 69: 234-240.
23. Schermer A, Galvin S & Sun TT (1986): Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol 103: 49-62.
24. Slettedal JK, Lyberg T, Ramstad H & Nicolaissen B (2006): Donor corneas for transplantation: A scanning electron microscopic study of the epithelium. Acta Ophthalmol Scand 84: 516-521.
25. Stulting RD, Waring GO III, Bridges WZ & Cavanagh HD (1988): Effect of donor epithelium on corneal transplant survival. Ophthalmology 95: 803-812.
26. Summerlin WT, Miller GE, Harris JE & Good RA (1973): The organ-cultured cornea: An in vitro study. Invest Ophthalmol 12: 176-180.
27. Sundmacher R (1985): The role of donor epithelium in perforating keratoplasty. Dev Ophthalmol 11: 61-67.
28. Thoft RA, Friend J, Freedman H & Dohlman CH (1975): Corneal epithelial preservation. Arch Ophthalmol 93: 357-361.
29. Tuberville AW, Foster CS & Wood TO (1983): The effect of donor cornea epithelium removal on the incidence of allograft rejection reactions. Ophthalmology 90: 1351-1356.
30. Van Horn DL, Doughman DJ, Harris JE, Miller GE, Lindstrom R & Good RA (1975): Ultrastructure of human organ-cultured cornea: II. Stroma and epithelium. Arch Ophthalmol 93: 275-277.
31. Zagon IS, Sassani JW, Ruth TB & McLaughlin PJ (2001): Epithelial adhesion complexes and organ culture of the human cornea. Brain Res 900: 205-213.