A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples

BMC Microbiology

Volumn 7, Issue , 2007, Pages

  Khairnar, Krishna ^a   Parija, Subhash C ^a  

^a JAWAHARLAL INSTITUTE OF POSTGRADUATE MEDICAL EDUCATION AND RESEARCH   (India)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PARASITE ANTIGEN; PROTOZOAL DNA;

ARTICLE; CONTROLLED STUDY; ENTAMOEBA; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; ENTAMOEBA MOSHKOVSKII; FECES ANALYSIS; MICROSCOPY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PARASITE CULTIVATION; PARASITE IDENTIFICATION; SENSITIVITY AND SPECIFICITY; AMEBIASIS; ANIMAL; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SPECIES DIFFERENCE;

ENTAMOEBA; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; ENTAMOEBA MOSHKOVSKII;

ANIMALS; ANTIGENS, PROTOZOAN; DNA, PROTOZOAN; ENTAMOEBA; ENTAMOEBA HISTOLYTICA; ENTAMOEBIASIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; HUMANS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY;

EID: 34250008184     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-7-47     Document Type: Article

References (27)

1. Amoebiasis. World Health Organization, WHO Weekly Epidemiol Rec 1997 72 97 100
2. Entamoeba moshkovskii infections in children, Bangladesh. IK Ali MB Hossain S Roy PF Ayeh-Kumi WA Petri Jr R Haque CG Calrk, Emerg Infect Dis 2003 9 580 584 12737742
3. Value of microscopy in the diagnosis of dysentery associated with invasive Entamoeba histolytica. A Gonzalez-Ruiz R Haque A Aguirre G Castanon A Hall F Guhl G Ruiz-Palacios MA Miles DC Warhurst, J Clin Pathol 1994 47 236 239 8163695
4. Amebiasis: epidemiologic studies in the United States, 1971-1974. DJ Krogstad HC Spencer Jr GR Healy NN Gleason DJ Sexton CA Herron, Ann Intern Med 1978 88 89 97 619763
5. Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. R Haque LM Neville P Hahn WA Petri Jr, J Clin Microbiol 1995 33 2558 2561 8567882
6. Molecular-based diagnosis of Entamoeba histolytica infection. CD Huston R Haque WA Petri Jr, Expert Rev Mol Med 1999 22 1 11 10.1017/S1462399499000599
7. Immunochromatographic strip-based detection of Entamoeba histolytica-E. dispar and Giardia lamblia coproantigen. DR Pillai KC Kain, J Clin Microbiol 1999 37 3017 3019 10449494
8. Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum antigens in human fecal specimens using the Triage parasite panel enzyme immunoassay. LS Garcia RY Shimizu CN Bernard, J Clin Microbiol 2000 38 3337 3340 10970380
9. Comparison of PCR, isoenzyme analysis, and antigen detection for diagnosis of Entamoeba histolytica infection. R Haque IKM Ali S Akther WA Petri Jr, J Clin Microbiol 1998 36 449 452 9466756
10. Entamoeba moshkovskii and Entamoeba dispar-associated Infections in Pondicherry, India. SC Parija K Khairnar, J Health Popul Nutr 2005 23 292 295 16262027
11. Laboratory diagnosis of amebiasis. M Tanyuksel WA Petri Jr, Clin Microbiol Rev 2003 16 713 729 14557296 10.1128/CMR.16.4.713-729.2003
12. Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples. YO Nunez MA Fernandez D Torres-Nunez JA Silva I Montano JL Maestre L Fonte, Am J Trop Med Hyg 2001 64 293 297 11463120
13. Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection. SJ Furrows AH Moody PL Chiodini, J Clin Pathol 2004 57 1264 1266 10.1136/jcp.2004.017822 15563665
14. Detection and differentiation of Entamoeba histolytica and Entamoeba dispar isolates in clinical samples by PCR and enzyme-linked immunosorbent assay. P Gonin L Trudel, J Clin Microbiol 2003 41 237 241 12517854 10.1128/JCM.41.1.237-241.2003
15. Evaluation of newer diagnostic methods for the detection and differentiation of Entamoeba histolytica in an endemic area. AK Sharma S Chibbar G Bansal U Kaur H Vohra, Trans R Soc Trop Med Hyg 2003 97 396 397 10.1016/S0035-9203(03)90068-X 15259465
16. Real-time-PCR assay for diagnosis of Entamoeba histolytica infection. S Roy M Kabir D Mondal IK Ali WA Petri Jr R Haque, J Clin Microbiol 2005 43 2168 2172 15872237 10.1128/JCM.43.5.2168-2172.2005
17. Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay. Z Hamzah S Petmitr M Mungthin S Leelayoova P Chavalitshewinkoon-Petmitr, J Clin Microbiol 2006 44 3196 200 16954247 10.1128/JCM.00778-06
18. Application of a Nested, Multiplex PCR to Psittacosis Outbreaks. TO Messmer SK Skelton JF Moroney H Daudharty BS Fields, J Clin Microbiol 1997 35 2043 2046 9230378
19. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis. J Sambrook DW Russell, Molecular Cloning, A Laboratory Manual New York, USA, Cold Spring Harbor Laboratory Press 3 2000 5.14 5.15
20. High rate of occult infection with Entamoeba histolytica among non-dysenteric Mexican children. OA Newton-Sanchez K Sturm-Ramirez JL Romero-Zamora JI Santos-Preciado J Samuelson, Arch Med Res 1997 28 S311 S313
21. Use of polymerase chain reaction and nonradioactive DNA probes to diagnose Entamoeba histolytica in clinical samples. JL Romero S Descoteaux S Reed E Orozco J Santos J Samuelson, Arch Med Res 1992 23 277 279 1340314
22. Evaluation of lacto-phenol cotton blue for wet mount preparation of feces. SC Parija PK Prabhakar, J Clin Microbiol 1995 33 1019 1021 7540624
23. Stool culture as a diagnostic aid in the detection of Entamoeba histolytica in the faecal specimens. SC Parija RS Rao, Indian J Pathol Microbiol 1995 38 359 363 9726144
24. Methods for cultivation of luminal parasitic protists of clinical importance. CG Clark LS Diamond, Clin Microbiol Rev 2002 15 329 41 12097242 10.1128/CMR.15.3.329-341.2002
25. The Laredo strain and other Entamoeba histolytica-like amoebae are Entamoeba moshkovskii. CG Clark LS Diamond, Mol Biochem Parasitol 1991 46 11 18 10.1016/0166-6851(91)90194-B 1677159
26. Primer3 Input. http://frodo.wi.mit.edu/
27. BLAST: Basic Local Alignment and Search Tool. http://www.ncbi.nlm.nih. gov/blast/